GIMM Flow Wiki

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<h2 style="color:#6586FF; margin-left:320px;">Imaging Flow Cytometry</h2>

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To operate our equipment you must attend the 3 hour session on the Principles of Flow Cytometry. If you haven’t already, please [[TrainingPrinciplesFlow|book your Session]]. 

We will schedule the practical training session on the imaging cell analyser, depending on the equipment availability and the planned date of your first experiment. Ideally, __you should be trained on the same day or a few days before your first usage__. 

After the training, you will be kept as an “in training” user on the Agendo booking system for the next few sessions and will be given full permission as soon as you are autonomously using the cytometer.
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====Be sure to read the following documents prior to your practical training:====

  * <html><a href="https://www.ttuhsc.edu/pharmacy/research/documents/Amnis-Imagestream-Manual.pdf" target="_blank"> INSPIRE Software</a></html>
  * <html><a href="https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.ucf.027_-_ideas_software_tutorial.pdf" target="_blank"> IDEAS Software</a></html>
  * <html><a href="https://www.medizin.uni-tuebingen.de/files/download/8YWbqX1VelmV0EKRLxkr9apM/IDEAS-User-Manual_6.2.pdf" target="_blank">IDEAS Software User Manual</a></html>

  * <html><a href="https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.ucf.004_-_ucf_imm_user_guidelines.pdf" target="_blank">User Guidelines</a></html>
  * <html><a href="https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=imagestreamx_sample_preparation_guide.pdf" target="_blank">Sample Preparation Guide</a></html> (for Cytek Amnis ImageStream)
  * <html><a href="https://imm.medicina.ulisboa.pt/facility/flowcytometry/doku.php?id=prepare_samples" target="_blank">How to prepare samples for Flow Cytometry</a></html> (General Flow Cytometry protocols)

====Check our Imaging Cytometer====
 
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[[newuser|Back to Training - Access to our resources]]
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[[start|Back to Flow Cytometry Wiki]]
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