GIMM Flow Wiki

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<h2 style="color:#6EA92D; margin-left:320px;">Polychromatic Flow Cytometry</h2>

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To operate our equipments you must attend the 3 hour session on the Principles of Flow Cytometry. If you haven’t already, please [[TrainingPrinciplesFlow|book your Session]]. 

We will schedule the practical training session on the cell analyser, depending on the equipment availability and the planned date of your first experiment. Ideally, __you should be trained a few days before your first acquisition__. 

After the training, you will be kept as an “in training” user on the Agendo booking system for the next few sessions and will be given full permission as soon as you are autonomously using the cytometer.
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====Be sure to read the following documents prior to your practical training:====

  * <html><a href="https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=SOP.UCF.011_-_BD_AccuriC6_Plus_Tutorial.pdf" target="_blank">Accuri Tutorial</a></html> (BD Accuri C6 Plus)
  * <html><a href="https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=SOP.UCF.003_-_BD_FACSDiva_Tutorial.pdf" target="_blank">DIVA Tutorial</a></html> (BD LSRFortessa, BD LSRFortessa X20)

  * <html><a href="https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.flow.994_-_good_practices_-_setting_and_sample_preparation.pdf" target="_blank"> SOP.FLOW.994 - Good Practices - Unstained and Single Color Controls Preparation</a></html>
  * <html><a href="https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.ucf.004_-_ucf_imm_user_guidelines.pdf" target="_blank">User Guidelines</a></html>
  * <html><a href="https://imm.medicina.ulisboa.pt/facility/flowcytometry/doku.php?id=prepare_samples" target="_blank">How to prepare samples for Flow Cytometry</a></html>

====Check our Conventional Analysers====
 
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<p style="text-align:left"><a href="https://imm.medicina.ulisboa.pt/facility/flowcytometry/doku.php?id=bd_accuri_c6" target="_blank"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=accuri_c6_plus_name_2.png"></a><a href="https://imm.medicina.ulisboa.pt/facility/flowcytometry/doku.php?id=bd_lsr_fortessa_2" target="_blank"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=fortessa_2_wiki_name_2.png"></a><a href="https://imm.medicina.ulisboa.pt/facility/flowcytometry/doku.php?id=bd_lsr_fortessa_sorp" target="_blank"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=fortessa_sorp_wiki_page_name.png"></a><a href="https://imm.medicina.ulisboa.pt/facility/flowcytometry/doku.php?id=bd_lsr_fortessa_x20" target="_blank"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=fortessa_x20_wiki_name_2.png"></a><a href="https://imm.medicina.ulisboa.pt/facility/flowcytometry/doku.php?id=bd_lsr_fortessa_x20_oeiras" target="_blank"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=fortessa_x20_wiki_name_oeiras.png"></a></p></td>
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