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<html><span style="color:#2E75B6;font-size:120%;">Panel Design Service</span></html>

Our Panel Design Service includes one-on-one consultations, tailored panel recommendations, and guidance on fluorophore selection based on antigen density, co-expression profiles, reagents available, and instrument compatibility. 
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By providing this support to researchers, we aim to significantly reduce troubleshooting, optimize experimental outcomes, and improve the overall quality of the data generated.

If you need help designing a **New Panel** or improving an existing one, please complete sheets 1–4 of the <html><a href="https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=form.flow.015_-_panel_design_1.xlsx" target="_blank">Panel Design Form</a></html> and send it to __flowcytometry@gimm.pt__.


Within the form, you will find the 8 steps required to design a new panel:
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**1. Run an unstained sample under the experimental conditions you will use in your analysis**
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This allows you to define the Autofluorescence (AF) spectrum of your cells so we can avoid fluorophores that fall on the same detectors.
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**2, 3. Design your Panel based on your biological question, animal model, tissue, and sample preparation conditions**
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Here you will define which markers to be used, their function, priority level, expression level, and co-express patterns. This information allows us to design your panel following Panel Design best practices and to provide you a tailored proposal.
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**4. Define the gating strategy for data analysis**
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**5. Evaluate similarity and Stain Index Reduction or Hotspot matrix**
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**6. Titrate all the antibodies to be used**
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After the titration of your antibodies, you are ready to do the settings on the instrument. Our team will be happy to support you during this session.
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When the settings on the instrument are done, we will:
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**7. Analyse the compensation/unmixing using a fully stained sample to validate the panel** 
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**8. Troubleshoot the compensation/unmixing, if necessary**
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====Important links:====

<html><a href="https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=form.flow.015_-_panel_design_1.xlsx" target="_blank">FORM.FLOW.015 - Panel Design</a></html>
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<html><a href="https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.flow.994_-_good_practices_-_setting_and_sample_preparation.pdf" target="_blank"> SOP.FLOW.994 - Good Practices - Settings and Sample Preparation</a></html>
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<html><a href="https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.flow.995_-_compensation_unmixing_troubleshooting.pdf" target="_blank"> SOP.FLOW.995 - Compensation and Unmixing Troubleshooting</a></html>

<html><a href="https://www.bdbiosciences.com/en-us/products/software/bd-research-cloud" target="_blank"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=bd_research_cloud.png" WIDTH=300></a></html>
<html><a href="https://cloud.cytekbio.com/login" target="_blank"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=cytek_cloud.png" width=300></a></html>

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