GIMM Flow Wiki

This page is read only. You can view the source, but not change it. Ask your administrator if you think this is wrong.
<html>
<img src="/facility/flowcytometry/lib/exe/fetch.php?media=fortessa_sorp_name_wiki_gimm.png" WIDTH=680>
<table style="border:0px solid white;">
<tr style="border:0px solid white;">
<td style="border:0px solid white;">
<img src="/facility/flowcytometry/lib/exe/fetch.php?media=fortessa_sorp_wiki.png"WIDTH=300>
<td style="border:0px solid white;"> <p style="line-height:1.8">
<b>Location</b>: Oeiras Site, Room 0B01, Bartolomeu Dias Wing, <br>(<img src="/facility/flowcytometry/lib/exe/fetch.php?media=telephone_icon.gif" width=15> 4625)<br> 
<b>Manufacturer</b>: <a href="http://www.bdbiosciences.com/eu/home" target="_blank">BD Biosciences</a><br>
<b>Model</b>: <a href="https://www.bdbiosciences.com/en-us/products/instruments/flow-cytometers/research-cell-analyzers/bd-lsrfortessa" target="_blank">LSRFortessa</a><br>
<b>Nickname</b>: "Fortessa SORP"<br>
<b>Software</b>: BD FACSDiva 9.0 <br>
<b>Year</b>: 2010 <br>
<b>SN</b>: H647900900001<br>
&nbsp <br>
&nbsp <br>
&nbsp <br>
&rarr; <a href="https://europe.agendoscience.com/calendar/?id=NjNjYWxlbmRhcnM=" target="_blank"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=booking.png">  BD LSRFortessa SORP Booking</a>
<br> 
&rarr; <a href="https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=bd_lsrfortessa_brochure.pdf" target="_blank"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=brochure.png">  BD LSRFortessa Brochure</a>
&nbsp </p>
</td>
</tr>
</table>
</html>
{{:info.gif|}} **Next Scheduled Maintenances**: 7 may and 28 November

===== System overview =====

{{  :flow_cell_sit_sample.png?0x300|}} The BD LSRFortessa //cell analyzer// is a benchtop digital flow cytometer equipped with a fixed-alignment flow cell that is gel-coupled to the collection optics. It has three spatially separated lasers - 442 nm, 488 nm and 561 nm. Its primary function is to characterize complex populations of cells. Individual cells pass single file through a stream, which is interrogated by the three lasers with the emitted light being detected by photomultiplier (PMT) tubes. The system electronics calculates the time it takes a single cell to pass through each laser intercept and assigns the signal of each PMT to the appropriate cell. Besides fluorescence, the physical properties of the cell are also analyzed with side scatter (SSC), which reflects the internal complexity of a cell and forward scatter (FSC), which is roughly proportional to cell size. The sample flow rate can be set to low, medium and high values, which correspond approximately to 12, 35 and 60 μl/minute, respectively. 

__Although the LSRFortessa SORP has a default configuration, it also allows the use of alternative configurations, taking advantage of a full set of optical filters and dichroic mirrors.__ This enables the optimization of the equipment to the project's requirements. See the default configuration, and the available set of filters bellow.

The LSRFortessa is equipped with a **High Throughput Sampler (HTS)**, which allows the automated acquisition of samples and is compatible with 96-well U, V, and flat-bottom plates as
well as 384-well microtiter plates. 


  * Three lasers: blue-violet (442 nm), blue (488 nm) and yellow-green (561 nm)
  * FSC-PMT for small particle analysis
  * Two SSC detectors
  * Multicolor analysis of up to 10 parameters
  * Flexible set of optical filters and dichroic mirrors, allowing the use of alternative configurations

===== Default Configuration =====

<html><img src="/facility/flowcytometry/lib/exe/fetch.php?media=bd_lsrfortessa_sorp_configuration.png"WIDTH=900></html>

===== Default Fluorophores Compatibility =====

<html><img src="/facility/flowcytometry/lib/exe/fetch.php?media=fortessa_sorp_fluorochromes.png"WIDTH=900><br><br>
</html>

<html><img src="/facility/flowcytometry/lib/exe/fetch.php?media=optical_filters_and_mirrors_fortessa_sorp.png"><br><br>
</html>

===== Useful Links when Preparing your Experiment =====

  * <html><a href="https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.ucf.005_-_how_to_prepare_samples_for_flow_cytometry.pdf" target="_blank"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=how_to_prepare_samples_for_flow_cytometry.png" WIDTH=400></a></html>
  * <html><a href="http://www.bdbiosciences.com/en-us/applications/research-applications/multicolor-flow-cytometry/product-selection-tools/spectrum-viewer" target="_blank"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=bd_spectrum_viewer.png" WIDTH=300></a></html>
  * <html><a href="http://www.bdbiosciences.com/en-us/applications/research-applications/multicolor-flow-cytometry/product-selection-tools/spectrum-viewer" target="_blank"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=bd_research_cloud.png" WIDTH=300></a></html>



===== Booking Rules =====

Available 7 days a week, 24 hours a day for trained researchers. 

----

[[resources|Back to the Equipment page]]
GIMM Flow Wiki

User Tools

Site Tools

Page Tools
