✎ bd_facsdiscover_s8 []

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<b>Location</b>: Lisbon Site, Room P2-A-49</a> (<img src="/facility/flowcytometry/lib/exe/fetch.php?media=telephone_icon.gif" width=15> 47224) <br> 
<b>Manufacturer</b>: <a href="http://www.bdbiosciences.com/eu/home" target="_blank">BD Biosciences</a><br>
<b>Model</b>: BD FACSDiscover S8<br>
<b>Nickname</b>: "S8"<br>
<b>Software</b>: BD FACSChorus <br>
<b>Year</b>: 2024 <br>
<b>SN</b>: <br>
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&rarr; <a href="https://europe.agendoscience.com/calendar/?id=MzMzY2FsZW5kYXJz" target="_blank"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=booking.png">  BD FACSDiscover S8 Booking</a>
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&rarr; <a href="/facility/flowcytometry/doku.php?id=bd_facsymphony_S6_usage"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=statistics.png"> BD FACSDiscover S8 Usage Statistics</a> 
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&rarr; <a href="https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=bd-facsdiscover-s8-cell-sorter-brochure.pdf" target="_blank"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=brochure.png"> BD FACSDiscover S8 Brochure</a>&nbsp
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{{:info.gif|}} **Next Scheduled Maintenances**:

{{:info.gif|}} [[s8_contamination|Pseudomonas contamination timeline]]

===== System overview =====

The BD FACSDiscover™ S8 cell sorter is a high speed benchtop digital flow cytometer equipped with a sensitive, fixed-alignment gel-coupled cuvette flow cell. It has five spatially separated lasers - 349 nm, 405 nm, 488 nm, 561 nm and 637 nm. Its primary function is to analyze complex populations of cells and yield pure populations of cells meeting defined criteria. The sorter can purify samples that are simply positive and negative for a single fluorophore or as complex as multi-color samples with complex gating strategies.

The BD FACSDiscover™ S8 allows for high-parameter spectral flow cytometry. It enables you to gain full visible spectrum coverage to collect all light emitted without the need for filter changes, increasing dye flexibility and number of simultaneous colors. It also allows for autofluorescence unmixing, that may enable the resolution of difficult cells and populations. Its __BD SpectralFX Technology__ combines full-spectrum optics, next gen QC and system-aware spectral unmixing that manages spread by adapting to instrument performance and sample conditions in real time. 

{{  :new_insights.png?600|}} The Cell Sorter leverages __BD CellView Image Technology__, a novel high-speed imaging technology that facilitates analysis and sorting through **real-time integration of image and flow data**.

Sorting can be performed with speeds up to 12,000 events/second. The system sorts by incorporating cells from the sample tube into a stream of sterile PBS. The stream is interrogated by the lasers at the flow cell and the system electronics keeps track of each cell as they pass through the laser and determines specific cells that meet the sort criteria. A transducer vibrates the stream and induces droplet formation, with cells in the stream being incorporated into the droplets. If a cell meets the sorting criteria and is in the last drop before the break off, the instrument will charge that drop. The charged droplet is then deflected into the proper collection tube by the charge plates. **The fully integrated Class II Type A2 biosafety cabinet meets personal and product protection, reducing the risk of operator exposure, while sorting potentially hazardous samples (e.g human samples and other BSL-2 level samples).**
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  * **Five lasers** with ultra-violet (349nm), violet (405 nm), blue (488 nm), yellow-green (561 nm) and red (637 nm) 
  * **78 fluorescent detectors**
  * **3 scatter detectors (Blue 488nm laser)**:
      * FSC
      * SSC
      * Axial Light Loss (ALL) 
  * **2 scatter detectors (Violet 405nm laser)**:
      * SSC
      * Axial Light Loss (ALL) 
  * __BD CellView Image Technology and BD SpectralFX Technology combining flow cytometry data with spatial and morphological insights.__
  * Combines **15 image features** with flow parameters for subset classification, statistical analysis and sorting
  * Resolves and provides image of 0.2-µm polystyrene particles from noise using at least one SSC parameter. 
  
  * 85 μm, 100 μm and 130 μm nozzles available
  * Up to 6-way sorting for 85 μm nozzle
  * Up to 4-way sorting for 100 μm nozzle
  * Up to 2-way sorting for 130 μm nozzle 
  
  * Tube sorting for 1.5 mL eppendorfs and 5 mL tubes (FACS tubes)
  * Plate sorting for 6-, 24-, 96-, 384-well, microscope slides and other custom plates

===== Configuration =====
<html><img src="/facility/flowcytometry/lib/exe/fetch.php?media=filter_guide.png"><br><img src="/facility/flowcytometry/lib/exe/fetch.php?media=s8_uv_laser.png"><br><img src="/facility/flowcytometry/lib/exe/fetch.php?media=s8_violet_laser.png"><br><img src="/facility/flowcytometry/lib/exe/fetch.php?media=s8_blue_laser.png"><br><img src="/facility/flowcytometry/lib/exe/fetch.php?media=s8_yellow_green_laser.png"><br><img src="/facility/flowcytometry/lib/exe/fetch.php?media=s8_red_laser.png"><br><img src="/facility/flowcytometry/lib/exe/fetch.php?media=s8_blue_imaging_laser.png"></html>
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===== Useful Links when Preparing your Experiment =====

  * <html><a href="https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.ucf.005_-_how_to_prepare_samples_for_flow_cytometry.pdf" target="_blank"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=how_to_prepare_samples_for_flow_cytometry.png" WIDTH=400></a></html>
  * <html><a href="http://www.bdbiosciences.com/en-us/applications/research-applications/multicolor-flow-cytometry/product-selection-tools/spectrum-viewer" target="_blank"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=bd_spectrum_viewer.png" WIDTH=300></a></html>
  * <html><a href="https://www.bdbiosciences.com/en-us/products/software/bd-research-cloud" target="_blank"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=bd_research_cloud.png" WIDTH=300></a></html>

===== Booking Rules =====

The system is available for booking from 10am to 6pm on Monday, Wednesday and Friday. 

The cell sorting service is provided by a dedicated technician upon reservation on the online booking system. 
The system can be booked up to 1 months in advance. 

Please check the <html><a href="https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.ucf.004_-_ucf_imm_user_guidelines_.pdf" target="_blank"> User Guidelines</a></html>  for detailed information.

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