✎ bd_facsaria_iiu []

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<b>Location</b>: <a href="/facility/flowcytometry/lib/exe/fetch.php?media=flow_cytometry_map.jpg">Room P2-A-49</a> (<img src="/facility/bioimaging/lib/exe/fetch.php?media=telephone_icon.gif" width=15> 47222) <br> 
<b>Manufacturer</b>: <a href="http://www.bdbiosciences.com/eu/home" target="_blank">BD Biosciences</a><br>
<b>Model</b>: FACSAria IIu<br>
<b>Software</b>: FACSDiva 6.1.3<br>
<b>Year</b>: 2005 <br>
<b>SN</b>: P22300027 <br>
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&rarr; <a href="https://imm.medicina.ulisboa.pt/intranet/booking/44"><img src="/facility/bioimaging/lib/exe/fetch.php?media=date.png">  BD FACSAria IIu Booking</a>
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&rarr; <a href="/facility/flowcytometry/doku.php?id=bd_facsaria_iiu_usage"><img src="/facility/bioimaging/lib/exe/fetch.php?media=chart_line.png">  BD FACSAria IIu Usage Statistics</a>
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===== System overview =====

{{  :cell_sorting.png?0x200|}} The BD FACSAria IIu //cell sorter// is a high speed benchtop digital flow cytometer equipped with a sensitive, fixed-alignment cuvette flow cell. It has three spatially separated lasers - 488 nm, 561 nm and 633 nm. Sorting can be performed into two or four tubes with speeds up to 25,000 events/second. Different cell types can be sorted with the use of 70 μm, 85 μm and 100 μm nozzles. The instrument is housed in a Baker BioProtect-IV bio-safety cabinet. 

  * Three lasers with blue (488 nm), yellow-green (561 nm) and red (633 nm)
  * Multicolor analysis of up to 10 parameters
  * 70 μm, 85 μm and 100 μm nozzles available
  * Up to 4-way sorting into 1.5 ml or 4 ml tubes and 2-way sorting into 15 ml tubes

===== Configuration =====

{{:bd_facsaria_iii_configuration_wiki.png?800|}}

===== Booking Rules =====

The system is available for booking from 10am to 10pm from Monday to Friday. 

The cell sorting service is provided by a dedicated technician upon reservation on the online booking system. 
The system can be booked up to 2 months in advance. 

Please check the [[https://intranet.imm.medicina.ulisboa.pt/c/document_library/get_file?p_l_id=183913&folderId=536842&name=DLFE-22602.pdf|user guidelines]] for detailed information.

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