GIMM Flow Wiki

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<b>Location</b>: Lisbon Site, Room P2-A-49</a> (<img src="/facility/flowcytometry/lib/exe/fetch.php?media=telephone_icon.gif" width=15> 47224) <br> 
<b>Manufacturer</b>: <a href="http://www.bdbiosciences.com/eu/home" target="_blank">BD Biosciences</a><br>
<b>Model</b>: <a href="https://www.bdbiosciences.com/en-us/products/instruments/flow-cytometers/research-cell-sorters/bd-facsaria-iii" target="_blank">FACSAria III</a><br>
<b>Nickname</b>: "Aria III"<br>
<b>Software</b>: BD FACSDiva 6.1.3<br>
<b>Year</b>: 2011 <br>
<b>SN</b>: P28200134 <br>
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&rarr; <a href="https://europe.agendoscience.com/calendar/?id=NDVjYWxlbmRhcnM=" target="_blank"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=booking.png">  BD FACSAria III Booking</a>
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&rarr; <a href="/facility/flowcytometry/doku.php?id=bd_facsaria_iii_usage"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=statistics.png">  BD FACSAria III Usage Statistics</a>
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&rarr; <a href="https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=bd-facsaria-iii-cell-sorter-brochure.pdf" target="_blank"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=brochure.png">  BD FACSAria III brochure</a>
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{{:info.gif|}} **Next Scheduled Maintenances**: 31 March and 15 September

{{:info.gif|}} [[aria_iii_contamination|Pseudomonas contamination timeline]]

===== System overview =====

{{  :cell_sorting.png?0x300|}} The BD FACSAria III //cell sorter// is a high speed benchtop digital flow cytometer equipped with a sensitive, fixed-alignment cuvette flow cell. It has four spatially separated lasers - 405 nm, 488 nm, 561 nm and 633 nm. Its primary function is to analyze complex populations of cells and yield pure populations of cells meeting defined criteria. The sorter can purify samples that are simply positive and negative for a single fluorophore or as complex as multi-color samples with complex gating strategies. 

Sorting can be performed into two or four tubes with speeds up to 25,000 events/second. The system sorts by incorporating cells from the sample tube into a stream of sterile PBS. The stream is interrogated by the lasers at the flow cell and the system electronics keeps track of each cell as they pass through the laser and determines specific cells that meet the sort criteria. A transducer vibrates the stream and induces droplet formation, with cells in the stream being incorporated into the droplets. If a cell meets the sorting criteria and is in the last drop before the break off, the instrument will charge that drop. The charged droplet is then deflected into the proper collection tube by the charge plates. Different cell types can be sorted with the use of 70 μm, 85 μm, 100 μm and 130 μm nozzles.

  * Four lasers - violet (405 nm), blue (488 nm), yellow-green (561 nm) and red (633 nm)
  * Multicolor analysis of up to 16 parameters
  * 70 μm, 85 μm, 100 μm and 130 μm nozzles available
  * Up to 4-way sorting into 1.5 ml, 2 mL eppendorfs and 5 mL tubes (FACS tubes)
  * 2-way sorting into 15 ml tubes
  * Sorting for 6-, 12-, 24-, 96-, 384-well and PCR plates

It shares the same optical system as the [[bd_lsr_fortessa_x20|BD LSRFortessa X20]] in <html><span style="color:blueviolet;font-size:100%;">Lisbon site</span></html> and the [[bd_lsr_fortessa_x20_oeiras|BD LSRFortessa X20]] in <html><span style="color:yellowgreen;font-size:100%;">Oeiras site</span></html>, allowing for the easy transfer of experiments from the analyzers (BD Accuri C6 Plus, BD LSRFortessa and BD LSRFortessa X-20) to the cell sorter without the need to modify your panels.

__If you need to sort potentially hazardous samples (e.g human samples and other BSL-2 level samples) you need to sort in our other sorters equiped with Class II Type A2 biosafety cabinets to reduce the risk of operator exposure.__

If you need high parameter cell sorting, check the [[bd_facsymphony_s6_se|BD FACSymphony S6 SE]] in <html><span style="color:blueviolet;font-size:100%;">Lisbon site</span></html> or the [[cytek_aurora_cs|CYTEK Aurora CS]] in <html><span style="color:Yellowgreen;font-size:100%;">Oeiras site</span></html>. 

===== Configuration =====

<html><img src="/facility/flowcytometry/lib/exe/fetch.php?media=bd_facsaria_iii_configuration_new_.png"WIDTH=900></html>

===== Fluorophores Compatibility =====

<html><img src="/facility/flowcytometry/lib/exe/fetch.php?media=facsaria_iii_fluorochrome_compatibility.png"WIDTH=900><br><br>
<img src="/facility/flowcytometry/lib/exe/fetch.php?media=aria_additional_filters.png"WIDTH=300><br><br>
<img src="/facility/flowcytometry/lib/exe/fetch.php?media=fortessa_x20_configuration_layouts.png"><br>
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===== Useful Links when Preparing your Experiment =====

  * <html><a href="https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.ucf.005_-_how_to_prepare_samples_for_flow_cytometry.pdf" target="_blank"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=how_to_prepare_samples_for_flow_cytometry.png" WIDTH=400></a></html>
  * <html><a href="http://www.bdbiosciences.com/en-us/applications/research-applications/multicolor-flow-cytometry/product-selection-tools/spectrum-viewer" target="_blank"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=bd_spectrum_viewer.png" WIDTH=300></a></html>
  * <html><a href="https://www.bdbiosciences.com/en-us/products/software/bd-research-cloud" target="_blank"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=bd_research_cloud.png" WIDTH=300></a></html>

===== Booking Rules =====

The system is available for booking from 10am to 6pm from Monday to Friday. The cell sorting service is provided by a dedicated technician upon reservation on the online booking system. 

The system is also available for booking for Power Users.

The system can be booked up to 1 months in advance. 

Please check the <html><a href="https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.ucf.004_-_ucf_imm_user_guidelines_.pdf" target="_blank"> User Guidelines</a></html>  for detailed information.

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