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--- usage_policy [2021/02/17 15:20]
flowcytometry
+++ usage_policy [2024/09/01 23:31] (current)
flowcytometry
@@ Line 1: / Line 1: @@
-<html><img src="/facility/flowcytometry/lib/exe/fetch.php?media=usage_rules.png"><BR><BR></html>
+===== Our Usage Rules =====
+\\
-Please download the detailed [[https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.ucf.004_-_ucf_imm_user_guidelines.pdf|User Guidelines]] for your own reference.
+Please download the detailed <html><a href="https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.ucf.004_-_ucf_imm_user_guidelines_.pdf" target="_blank"> User Guidelines </a></html> for your own reference.
-===== Cell Analyzers =====
+==== Cell Analyzers ====
-  - Users must get trained on a cell analyzer before using it. Use the booking system or [[imm-flowcytometry@medicina.ulisboa.pt|email us]] to request training.
+  - Users must get trained on a cell analyzer before using it. Follow the [[newuser|New User guidelines]] to start using our equipment.
-  - The cell analyzers **can be booked up to 2 months in advance**
+  - The cell analyzers **can be booked up to 1 month in advance**.
-  - Users must **include 15 min in their booking time for cleaning**
+  - Users must **include 15 min in their booking time for cleaning**.
   - Users must have a valid reservation in a system before using it.
   - Users must use their own booked time and not book or use a resource with other username.
-  - Users **can only cancel bookings 3h before the slot starting time**
+  - Users **can only cancel bookings 3h before the slot starting time**.
-  - Users must record start and finish times on the system log sheet (cleaning time included) and problems (if any)
+  - Users must perform an additional cleaning before acquisition if they are the **first user of the day**.
-  - Users that swap filters must leave the system with the default configuration
+  - Users must record start and finish times on the system log sheet (cleaning time included) and problems (if any).
-  - Users must log out from the analyzer software at the end of their session
+  - Users that swap filters must leave the system with the default configuration.
-  - Users must **switch off the system** if they are the **last user booked** or if the analyzer **will not be used for at least 3h**
+  - Users must **always clean the machines** after acquisition following the cleaning protocol and **record the cleaning steps** in the Software.
-  - Users must always export their data and delete it from the FACSDiva database regularly
-  - Users must **always clean the machines** after acquisition following the cleaning protocol and **record the cleaning steps** in FACSDiva
-  - Users must perform an additional cleaning before acquisition if they are the **first user of the day**
   - Users must **refill the sheath tank** with PBS+Azide at the end of the booked session.
   - If during acquisition, the **waste container gets full**, a high pitch sound will go off. Users should **disconnect the black sensor** (the noise will stop), put the cytometer in **Standby**, and **disconnect
 the orange connector.** The container must be replaced with a new one.
+  - Users must always export their data and delete it from the Software database regularly.
+  - Users must log out from the analyzer software at the end of their session.
+  - (BD LSRFortessa, LSRFortessa SORP and Fortessa X-20) Users must **switch off the system** if they are the **last users booked** or if the analyzer **will not be used for at least 3h**.
+  - (BD Accuri C6 Plus, BD FACSymphony A5 SE and Aurora) After 5pm or on weekends and holidays, users must **switch off the system** if they are the **last users booked**.
 {{warning.jpg?20|}} Users will be **billed according to the actual starting time or booked starting time (whichever occurs first) and the actual ending time or booked ending time (whichever occurs last)**
@@ Line 28: / Line 29: @@
 {{warning.jpg?20|}} ** NO SHOWS** - **the charge corresponds to the running cost of the system**
-===== Cell Sorters =====
+==== Cell Sorters ====
-  - Users must [[imm-flowcytometry@medicina.ulisboa.pt|contact us]] or use the booking system to request access to the cell sorters booking.
+  - Users must follow the [[newuser|New User guidelines]] to start using our cell sorters.
-  - The cell sorters **can be booked up to 2 months in advance** between 10am and 6pm
+  - The cell sorters **can be booked up to 1 month in advance** starting from 10am until 5pm (Oeiras Site) or 6pm (Lisbon Site).
-  - If you need to book a sorter outside the regular working hours, please contact us beforehand to check for staff availability.
+  - If you need to book a sorter outside the regular working hours, please contact the Head of Facility beforehand to check staff availability.
-  - Users must **include time in their booking for new settings, purity checks and cleaning** (minimum 30 min)
+  - Users must **include time in their booking for new settings, purity checks and cleaning** (minimum 30 min).
-  - Users must mention in the booking comments which nozzle (70 μm, 85 μm or 100 μm) and collection tubes (1.5 mL tubes, 5 mL FACS tubes or 15 mL Falcon tubes) they will be using
+  - Users must mention in the booking comments the Biosafety Level (BSL) of their samples, which nozzle (70 μm, 85 μm, 100 μm or 130 μm) and which collection devices (plates, 1.5 mL tubes, 5 mL FACS tubes, 15 mL Falcon tubes or plates) they will be using.
-  - Users must not book on behalf of others
+  - Users must not book on behalf of others.
-  - Users **can only cancel bookings 7 days in advance**
+  - Users **can only cancel bookings 7 days in advance**.
-{{warning.jpg?20|}} ** Cancellations** (within 6 days in advance) may result in a penalization \\
+{{warning.jpg?20|}} ** Cancellations** (within 7 days in advance) may result in a penalization \\
 **The penalty charge corresponds to the running cost of the system if the session is not booked by another user**
 \\
-{{warning.jpg?20|}} ** NO SHOWS** (or cancellation in the booked day) - **the charge corresponds to the running cost of the system**
+{{warning.jpg?20|}} ** NO SHOWS**  - **the charge corresponds to the running cost of the system**
-==== Sample Preparation ====
-Please check **[[prepare_samples|How to prepare samples for Flow Cytometry]]**.
 ===== Abuse Report =====
-If you find a user that did not comply with the above rules please [[imm-flowcytometry@medicina.ulisboa.pt|contact us]].
+If you find a user that did not comply with the above rules please [[mariana.fernandes@medicina.ulisboa.pt|contact us]].
 ----
 [[start|Back to the Flow Cytometry Wiki]]

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