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tutorials [2024/08/23 11:05]
flowcytometry
tutorials [2026/04/08 18:43] (current)
flowcytometry
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 <​html><​BR></​html>​ <​html><​BR></​html>​
 +
 +===== Planning Tools =====
 +\\
 +<​html><​img src="/​facility/​flowcytometry/​lib/​exe/​fetch.php?​media=experimental_planning.png"><​BR><​BR></​html>​
 +<​html><​a href="​https://​imm.medicina.ulisboa.pt/​facility/​flowcytometry/​lib/​exe/​fetch.php?​media=sop.ucf.005_-_how_to_prepare_samples_for_flow_cytometry.pdf"​ target="​_blank">​ How to prepare samples for Flow Cytometry</​a></​html>​
 +
 +<​html><​a href="​https://​imm.medicina.ulisboa.pt/​facility/​flowcytometry/​lib/​exe/​fetch.php?​media=sop.ucf.015_-_tracking_settings_with_rainbow_beads.pdf"​ target="​_blank">​ Tracking settings with Rainbow Beads</​a></​html>​
 +
 +\\
 +<​html><​img src="/​facility/​flowcytometry/​lib/​exe/​fetch.php?​media=spectrum_viewers.png"><​BR><​BR></​html>​
 +<​html><​a href="​http://​www.thermofisher.com/​order/​fluorescence-spectraviewer#​!/"​ target="​_blank">​ThermoFisher Spectrum viewer</​a></​html>​
 +
 +<​html><​a href="​http://​www.bdbiosciences.com/​en-us/​applications/​research-applications/​multicolor-flow-cytometry/​product-selection-tools/​spectrum-viewer"​ target="​_blank">​BD Spectrum viewer</​a></​html>​
 +
 +<​html><​a href="​https://​www.biolegend.com/​spectraanalyzer"​ target="​_blank">​Biolegend Spectrum viewer</​a></​html>​
 +
 +<​html><​a href="​https://​spectrum.cytekbio.com/"​ target="​_blank">​Cytek (Full) Spectrum viewer</​a></​html>​
 +
 +\\
 +<​html><​img src="/​facility/​flowcytometry/​lib/​exe/​fetch.php?​media=multicolor_panel_design.png"><​BR><​BR></​html>​
 +<​html><​a href="​https://​www.bdbiosciences.com/​en-us/​support/​reagent_education"​ target="​_blank">​BD Panel Design Education</​a></​html>​
 +
 +<​html><​a href="​https://​imm.medicina.ulisboa.pt/​facility/​flowcytometry/​lib/​exe/​fetch.php?​media=sop.ucf.006_-_panel_design.pdf"​ target="​_blank">​Tips for Panel Design</​a></​html>​
 +
 +<​html><​a href="​http://​www.bu.edu/​flow-cytometry/​files/​2014/​09/​Fluorochrome-Chart-Relative-Brightness.pdf"​ target="​_blank">​BD Relative Fluorochrome Brightness</​a></​html>​
 +
 +<​html><​a href="​https://​imm.medicina.ulisboa.pt/​facility/​flowcytometry/​lib/​exe/​fetch.php?​media=bd_fluorochrome_relative_brightness.pdf"​ target="​_blank">​BD Fluorochrome Relative Brightness (5-laser FACSymphony)</​a></​html>​
 +
 +<​html><​a href="​https://​www.cincinnatichildrens.org/​-/​media/​cincinnati%20childrens/​home/​research/​cores/​flow-cytometry/​analyzer/​aurora.pdf?​la=en"​ target="​_blank">​Cytek Staining Index Rankings - Fluorochromes Selection Guide</​a></​html>​
 +
 +<​html><​a href="​https://​imm.medicina.ulisboa.pt/​facility/​flowcytometry/​lib/​exe/​fetch.php?​media=Cytek_Fluorochrome_StainIndex_Ranking.pdf"​ target="​_blank">​Cytek Fluorochrome Stain Index Ranking (5-laser Aurora)</​a></​html>​
 +
 +<​html><​a href="​https://​cytekbio.com/​blogs/​spectrolearn"​ target="​_blank">​Cytek SpectroLearn - Full Spectrum Profiling™ Technology (FSP<​sup>​TM</​sup>​) Panel Design Series</​a></​html>​
 +
 +\\
 +<​html><​img src="/​facility/​flowcytometry/​lib/​exe/​fetch.php?​media=designed_multicolor_panels.png"><​BR><​BR></​html>​
 +
 +<​html><​a href="​https://​fluorofinder.com/​omips/"​ target="​_blank">​**OMIPs (Optimized Multicolor Immunofluorescence Panels)**</​a></​html>​
 +\\
 +\\
 +==Fortessa/​Fortessa X20==
 +<​html><​a href="​http://​static.bdbiosciences.com/​documents/​Flow-Cytometry-Reagents-PS-Panels-with-Fortessa-X20.pdf?​_ga=1.148042303.945356473.1478101143"​ target="​_blank">​Multicolor Panels Designed for Optimal Resolution</​a></​html>​
 +
 +<​html><​a href="​http://​static.bdbiosciences.com/​documents/​X20-Protocol-System-5-Color-Panels.pdf?​_ga=1.82680222.945356473.1478101143"​ target="​_blank">​5-Color Panels Designed for Minimal Compensation</​a></​html>​
 +
 +<​html><​a href="​http://​static.bdbiosciences.com/​documents/​X20-Protocol-4-Color-Panels-Minimal-Spectral-Overlap.pdf?​_ga=1.111997196.945356473.1478101143"​ target="​_blank">​4-Color Panels Minimal Spectral Overlap</​a></​html>​
 +
 +<​html><​a href="​http://​static.bdbiosciences.com/​documents/​X20-Protocol-15-Color-Immunophenotyping.pdf?​_ga=1.82680222.945356473.1478101143"​ target="​_blank">​15-Color Immunophenotyping</​a></​html>​
 +\\
 +\\
 +==BD FACSymphony A5 SE/CYTEK Aurora==
 +<​html><​a href="​https://​welcome.cytekbio.com/​hubfs/​Website%20Downloadable%20Content/​Publications/​OMIP_069_CYTEK.pdf"​ target="​_blank">​40-Color Immunophenotyping (OMIP-069)</​a></​html>​
 +
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 [[start|Back to the Flow Cytometry Wiki]] [[start|Back to the Flow Cytometry Wiki]]
tutorials.txt · Last modified: 2026/04/08 18:43 by flowcytometry