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trainingselfsorting [2024/02/22 22:47] flowcytometry |
trainingselfsorting [2026/05/08 15:33] (current) flowcytometry |
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| - | {{self_cell_sorting_banner.png }} | + | <html> |
| - | \\ | + | <h2 style="color:#E1B205; margin-left:320px;">Power User Cell Sorting</h2> |
| - | \\ | + | <img src="/facility/flowcytometry/lib/exe/fetch.php?media=self_cell_sorting_banner.png" |
| - | \\ | + | style="width:300px; height:auto; float:left; margin-right:20px; margin-bottom:10px;"> |
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| + | </html> | ||
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| **How to become a power user** | **How to become a power user** | ||
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| * Users who often need to sort outside of service hours (e.g. unexpected patient samples, which have to be processed immediately). | * Users who often need to sort outside of service hours (e.g. unexpected patient samples, which have to be processed immediately). | ||
| * Technicians from research groups who are willing to handle all sorts of the whole research group. | * Technicians from research groups who are willing to handle all sorts of the whole research group. | ||
| - | + | __ | |
| - | All requests for cell sorter training have to be approved by the facility head. | + | All requests for cell sorter training have to be approved by the facility Head. |
| + | __ | ||
| **Prerequisite for sorter training** | **Prerequisite for sorter training** | ||
| - | We expect that you are able to operate an analyzer (BD LSR Fortessa or BD LSR Fortessa X-20) independently. We observed that users without practical experience in FACS Diva software have massive difficulties during the first training sessions. If you were trained on a different instrument, we highly recommend using one of our analyzers to make yourself familiar with this software before the start of the sorter training. | + | We expect that you are able to operate an analyzer (BD LSRFortessa or BD LSRFortessa X-20) independently. We observed that users without practical experience in FACS Diva software have massive difficulties during the first training sessions. If you were trained on a different instrument, we highly recommend using one of our analyzers to make yourself familiar with this software before the start of the sorter training. |
| **Training** | **Training** | ||
| - | To operate our equipments you must attend the 2,5 hour session on the Principles of Flow Cytometry. If you haven’t already, please [[TrainingPrinciplesFlow|book your Session]]. | + | To operate our equipments you must attend the 3 hour session on the Principles of Flow Cytometry. If you haven’t already, please [[TrainingPrinciplesFlow|book your Session]]. |
| - | Training to self-operate the sorters requires attending a __second theoretical session on the Principles of Cell Sorting.__ | + | Training to self-operate the sorters requires attending a __second theoretical session on the Principles of Cell Sorting. |
| + | __ | ||
| + | Please contact us to discuss your experiments and the options available. | ||
| This 2 hour session is open to anyone who wishes to understand how to sort, optimize their sorting experiments, or simply to improve their cell sorting skills. It covers the fundamental principles of FACS (Fluorescence Activated Cell Sorting), and all the concepts for better planning, executing, and analyzing the sorting experiments. | This 2 hour session is open to anyone who wishes to understand how to sort, optimize their sorting experiments, or simply to improve their cell sorting skills. It covers the fundamental principles of FACS (Fluorescence Activated Cell Sorting), and all the concepts for better planning, executing, and analyzing the sorting experiments. | ||
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| - | <p style="text-align:left"><a href="https://imm.medicina.ulisboa.pt/facility/flowcytometry/doku.php?id=bd_facsaria_fusion" target="_blank"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=fusion_wiki_name_2.png" width=150></a></p></td> | + | <p style="text-align:left"><a href="https://imm.medicina.ulisboa.pt/facility/flowcytometry/doku.php?id=bd_facsaria_iii" target="_blank"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=aria iii wiki new name 2 2.png"></a><a href="https://imm.medicina.ulisboa.pt/facility/flowcytometry/doku.php?id=bd_facsaria_fusion" target="_blank"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=fusion_wiki_name_2_2.png"></a></p></td> |
| </html> | </html> | ||
| -------- | -------- | ||
| - | [[newuser|Back to New User - Access to our resources]] | + | [[newuser|Back to Training - Access to our resources]] |
| + | \\ | ||
| + | [[about|Back to Our Platform]] | ||
| + | \\ | ||
| + | [[start|Back to Flow Cytometry Wiki]] | ||