bd_lsr_fortessa_x20
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bd_lsr_fortessa_x20 [2021/02/04 11:26] flowcytometry |
bd_lsr_fortessa_x20 [2025/02/14 19:31] (current) flowcytometry |
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| <html> | <html> | ||
| - | <img src="/facility/flowcytometry/lib/exe/fetch.php?media=bd_lsrfortessa_x-20_name.jpg"> | + | <img src="/facility/flowcytometry/lib/exe/fetch.php?media=fortessa_x20_name_wiki_lisbon_gimm.png"> |
| <table style="border:0px solid white;"> | <table style="border:0px solid white;"> | ||
| <tr style="border:0px solid white;"> | <tr style="border:0px solid white;"> | ||
| <td style="border:0px solid white;"> | <td style="border:0px solid white;"> | ||
| - | <a href="/facility/flowcytometry/lib/exe/fetch.php?media=fortessa_x20_wiki.png"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=fortessa_x20_wiki.png" width=300></a> | + | <img src="/facility/flowcytometry/lib/exe/fetch.php?media=fortessa_x20_wiki_2.jpg"WIDTH=300> |
| <td style="border:0px solid white;"> <p style="line-height:1.8"> | <td style="border:0px solid white;"> <p style="line-height:1.8"> | ||
| - | <b>Location</b>: <a href="/facility/flowcytometry/lib/exe/fetch.php?media=flow_cytometry_map.jpg">Room P2-A-49</a> (<img src="/facility/flowcytometry/lib/exe/fetch.php?media=telephone_icon.gif" width=15> 47224) <br> | + | <b>Location</b>: Lisbon Site, Room P2-A-49</a> (<img src="/facility/flowcytometry/lib/exe/fetch.php?media=telephone_icon.gif" width=15> 47224) <br> |
| <b>Manufacturer</b>: <a href="http://www.bdbiosciences.com/eu/home" target="_blank">BD Biosciences</a><br> | <b>Manufacturer</b>: <a href="http://www.bdbiosciences.com/eu/home" target="_blank">BD Biosciences</a><br> | ||
| - | <b>Model</b>: <a href="http://www.bdbiosciences.com/us/instruments/research/cell-analyzers/bd-lsrfortessa-x-20/m/1519232/overview" target="_blank">LSRFortessa X-20</a><br> | + | <b>Model</b>: <a href="https://www.bdbiosciences.com/en-us/products/instruments/flow-cytometers/research-cell-analyzers/bd-lsrfortessa-x-20" target="_blank">LSRFortessa X-20</a><br> |
| - | <b>Nickname</b>: "X20"<br> | + | <b>Nickname</b>: "X20 Lisbon"<br> |
| <b>Software</b>: FACSDiva 8.0<br> | <b>Software</b>: FACSDiva 8.0<br> | ||
| <b>Year</b>: 2017 <br> | <b>Year</b>: 2017 <br> | ||
| Line 16: | Line 16: | ||
|   <br> |   <br> | ||
|   <br> |   <br> | ||
| - | → <a href="https://my.agendo.science/calendar/?id=NDJjYWxlbmRhcnM="><img src="/facility/flowcytometry/lib/exe/fetch.php?media=date.png"> BD LSRFortessa X-20 Booking</a> | + | → <a href="https://europe.agendoscience.com/calendar/?id=NDJjYWxlbmRhcnM=" target="_blank"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=booking.png"> BD LSRFortessa X-20 Booking</a> |
| <br> | <br> | ||
| - | → <a href="/facility/flowcytometry/doku.php?id=bd_lsrfortessa_x20_usage"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=chart_line.png"> BD LSRFortessa X-20 Usage Statistics</a> | + | → <a href="/facility/flowcytometry/doku.php?id=bd_lsrfortessa_x20_usage"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=statistics.png"> BD LSRFortessa X-20 Usage Statistics</a> |
| - |   | + | <br> |
| - | <br> | + | → <a href="https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=bd_lsr_fortessax20_brochure.pdf" target="_blank"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=brochure.png"> BD LSRFortessa X-20 Brochure</a> |
| - | → <a href="/facility/flowcytometry/doku.php?id=bd_lsrfortessa_x20_repair"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=repair-icon.gif" width=16> BD LSRFortessa X-20 Repair History</a></p> | + | |
| </td> | </td> | ||
| </tr> | </tr> | ||
| </table> | </table> | ||
| </html> | </html> | ||
| - | {{:info.gif|}} **Next Scheduled Maintenances**: 23 February and 24 August, 2021 | + | {{:info.gif|}} **Next Scheduled Maintenances**: 26 February and 20 August |
| ===== System overview ===== | ===== System overview ===== | ||
| - | {{ :cell_analyzers.png?0x300|}} The BD LSRFortessa X-20 //cell analyzer// is a benchtop digital flow cytometer equipped with a fixed-alignment flow cell that is gel-coupled to the collection optics. It has four spatially separated lasers - 405 nm, 488 nm, 561 nm and 640 nm. Its primary function is to characterize complex populations of cells. Individual cells pass single file through a stream, which is interrogated by the four lasers with the emitted light being detected by photomultiplier (PMT) tubes. The system electronics calculates the time it takes a single cell to pass through each laser intercept and assigns the signal of each PMT to the appropriate cell. Besides fluorescence, the physical properties of the cell are also analyzed with side scatter (SSC), which reflects the internal complexity of a cell and forward scatter (FSC), which is roughly proportional to cell size. The sample flow rate can be set to low, medium and high values, which correspond approximately to 12, 35 and 60 μl/minute, respectively. If you don't need multicolor analysis of up to 16 parameters, check the [[bd_lsr_fortessa_1|BD LSRFortessa 1]] instead. If you need a high-throughput sampler, check the [[bd_lsr_fortessa_2|BD LSRFortessa 2]]. | + | {{ :flow_cell_sit_sample.png?0x300|}} The BD LSRFortessa X-20 //cell analyzer// is a benchtop digital flow cytometer equipped with a fixed-alignment flow cell that is gel-coupled to the collection optics. It has four spatially separated lasers - 405 nm, 488 nm, 561 nm and 640 nm. Its primary function is to characterize complex populations of cells. Individual cells pass single file through a stream, which is interrogated by the four lasers with the emitted light being detected by photomultiplier (PMT) tubes. The system electronics calculates the time it takes a single cell to pass through each laser intercept and assigns the signal of each PMT to the appropriate cell. Besides fluorescence, the physical properties of the cell are also analyzed with side scatter (SSC), which reflects the internal complexity of a cell and forward scatter (FSC), which is roughly proportional to cell size. The sample flow rate can be set to low, medium and high values, which correspond approximately to 12, 35 and 60 μl/minute, respectively. |
| + | * Four lasers with violet (405 nm), blue (488 nm), yellow-green (561 nm) and red (640 nm) | ||
| * Multicolor analysis of up to 16 parameters | * Multicolor analysis of up to 16 parameters | ||
| - | * Four lasers with violet (405 nm), blue (488 nm), yellow-green (561 nm) and red (640 nm) | + | |
| + | If you need multicolor analysis of more than 16 parameters, check the [[bd_facs_symphony_a5_se|BD FACSymphony A5 SE]] or the [[cytek_aurora|CYTEK Aurora]] in <html><span style="color:blueviolet;font-size:100%;">Lisbon site</span></html> and the [[cytek_aurora_oeiras|CYTEK Aurora]] in <html><span style="color:yellowgreen;font-size:100%;">Oeiras site</span></html>. | ||
| + | |||
| + | If you need a high-throughput sampler, check the [[bd_lsr_fortessa_2|BD LSRFortessa]] or the [[cytek_aurora|CYTEK Aurora]] in <html><span style="color:blueviolet;font-size:100%;">Lisbon site</span></html> and the [[cytek_aurora_oeiras|CYTEK Aurora]] or the [[bd_lsr_fortessa_sorp|BD LSRFortessa SORP]] in <html><span style="color:yellowgreen;font-size:100%;">Oeiras site</span></html>. | ||
| ===== Configuration ===== | ===== Configuration ===== | ||
| - | {{:bd_lsrfortessa_x-20_configuration_wiki_1.png?700|}} | + | <html><img src="/facility/flowcytometry/lib/exe/fetch.php?media=bd_lsrfortessa_x-20_configuration_new_.png"WIDTH=900></html> |
| \\ | \\ | ||
| - | {{:bd_lsrfortessa_x-20_configuration_wiki_2.png?700|}} | ||
| - | |||
| ===== Fluorophores Compatibility ===== | ===== Fluorophores Compatibility ===== | ||
| - | {{{{:fortessa_x20_fluorochomes.png?800|}} | + | <html><img src="/facility/flowcytometry/lib/exe/fetch.php?media=fortessa_x20_fluorochromes.png"WIDTH=900><br><br> |
| - | \\ | + | <img src="/facility/flowcytometry/lib/exe/fetch.php?media=fortessa_x20_additional_filters.png"WIDTH=250><br><br> |
| - | {{:fortessa_x20_additional_filters.png?210|}} | + | <img src="/facility/flowcytometry/lib/exe/fetch.php?media=bd_lsrfortessa_x-20_405.png"WIDTH=700><br> |
| - | \\ | + | <img src="/facility/flowcytometry/lib/exe/fetch.php?media=bd_lsrfortessa_x-20_488.png"WIDTH=700><br> |
| - | \\ | + | <img src="/facility/flowcytometry/lib/exe/fetch.php?media=bd_lsrfortessa_x-20_561.png"WIDTH=700><br> |
| - | {{::bd_lsrfortessa_x-20_405.png?700|}} | + | <img src="/facility/flowcytometry/lib/exe/fetch.php?media=bd_lsrfortessa_x-20_640.png"WIDTH=700> |
| - | \\ | + | </html> |
| - | {{::bd_lsrfortessa_x-20_488.png?700|}} | + | |
| - | \\ | + | ===== Useful Links when Preparing your Experiment ===== |
| - | {{::bd_lsrfortessa_x-20_561.png?700|}} | + | |
| - | \\ | + | * <html><a href="https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.ucf.005_-_how_to_prepare_samples_for_flow_cytometry.pdf" target="_blank"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=how_to_prepare_samples_for_flow_cytometry.png" WIDTH=400></a></html> |
| - | {{::bd_lsrfortessa_x-20_640.png?700|}} | + | * <html><a href="http://www.bdbiosciences.com/en-us/applications/research-applications/multicolor-flow-cytometry/product-selection-tools/spectrum-viewer" target="_blank"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=bd_spectrum_viewer.png" WIDTH=300></a></html> |
| + | * <html><a href="http://www.bdbiosciences.com/en-us/applications/research-applications/multicolor-flow-cytometry/product-selection-tools/spectrum-viewer" target="_blank"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=bd_research_cloud.png" WIDTH=300></a></html> | ||
| ===== Additional Information ===== | ===== Additional Information ===== | ||
| - | * **[[prepare_samples|How to prepare samples for Flow Cytometry]]** | + | * [[https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.ucf.022_-_bd_lsrfortessa_x-20_operation.pdf| BD LSRFortessa X-20 Operation]] |
| - | * [[https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=form.ucf.039_-_bd_lsrfortessa_x-20_operation.pdf| BD LSRFortessa X-20 Operation]] | + | * [[https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.ucf.003_-_bd_facsdiva_tutorial.pdf| BD FACSDiva Software Tutorial]] |
| - | * [[https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=form.ucf.047_-_bd_lsrfortessa_troubleshooting.pdf| BD LSRFortessa Troubleshooting]] | + | * [[https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.ucf.024_-_bd_lsrfortessa_troubleshooting.pdf| BD LSRFortessa Troubleshooting]] |
| + | * [[https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.ucf.025_-_bd_lsrfortessa_cleaning.pdf| BD LSRFortessa Cleaning]] | ||
| + | * [[https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.ucf.030_-_bd_lsrfortessa_X20_starting_up_cleaning.pdf| BD LSRFortessa X20 Starting up Cleaning]] for the first user of the day, on weekends and out of staff working hours | ||
| - | ===== Sample Preparation ===== | ||
| - | |||
| - | * [[http://static.bdbiosciences.com/documents/Flow-Cytometry-Reagents-PS-Panels-with-Fortessa-X20.pdf?_ga=1.148042303.945356473.1478101143|Multicolor Panels Designed for Optimal Resolution ]] | ||
| - | * [[http://static.bdbiosciences.com/documents/X20-Protocol-15-Color-Immunophenotyping.pdf?_ga=1.82680222.945356473.1478101143|15-Color Immunophenotyping]] | ||
| - | * [[http://static.bdbiosciences.com/documents/X20-Protocol-System-5-Color-Panels.pdf?_ga=1.82680222.945356473.1478101143|5-Color Panels Designed for Minimal Compensation]] | ||
| - | * [[http://static.bdbiosciences.com/documents/X20-Protocol-4-Color-Panels-Minimal-Spectral-Overlap.pdf?_ga=1.111997196.945356473.1478101143|4-Color Panels Minimal Spectral Overlap]] | ||
| - | * [[http://static.bdbiosciences.com/documents/X20-Protocol-8-Color-Panels-for-Mesenchymal-Stromal-Cells.pdf?_ga=1.111997196.945356473.1478101143|8-Color Panels for Mesenchymal Stromal Cells]] | ||
| ===== Booking Rules ===== | ===== Booking Rules ===== | ||
| Line 75: | Line 72: | ||
| Available 7 days a week, 24 hours a day for trained researchers. | Available 7 days a week, 24 hours a day for trained researchers. | ||
| - | Please check the {{ ::sop.ucf.004_-_ucf_imm_user_guidelines.pdf |User Guidelines}} for detailed information. | + | Please check the <html><a href="https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.ucf.004_-_ucf_imm_user_guidelines_.pdf" target="_blank"> User Guidelines</a></html> for detailed information. |
| - | + | ||
| - | ===== Cleaning Protocol ===== | + | |
| - | + | ||
| - | {{::cleaning_procedure_fortessa.png?700|}} | + | |
| ---- | ---- | ||
| - | [[resources|Back to the Resources page]] | + | [[resources|Back to the Equipment page]] |
bd_lsr_fortessa_x20.1612434376.txt.gz · Last modified: 2021/02/04 11:26 by flowcytometry
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