bd_facsymphony_s6_se
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bd_facsymphony_s6_se [2024/08/22 18:09] flowcytometry |
bd_facsymphony_s6_se [2025/01/03 16:19] (current) flowcytometry |
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| - | <a href="/facility/flowcytometry/lib/exe/fetch.php?media=symphony_s6_wiki.png"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=symphony_s6_wiki.png" width=300></a> | + | <img src="/facility/flowcytometry/lib/exe/fetch.php?media=symphony_s6_wiki.png"WIDTH=300> |
| <td style="border:0px solid white;"> <p style="line-height:1.8"> | <td style="border:0px solid white;"> <p style="line-height:1.8"> | ||
| - | <b>Location</b>: Lisbon site: Room P2-A-49</a> (<img src="/facility/flowcytometry/lib/exe/fetch.php?media=telephone_icon.gif" width=15> 47224) <br> | + | <b>Location</b>: Lisbon Site, Room P2-A-49</a> (<img src="/facility/flowcytometry/lib/exe/fetch.php?media=telephone_icon.gif" width=15> 47224) <br> |
| <b>Manufacturer</b>: <a href="http://www.bdbiosciences.com/eu/home" target="_blank">BD Biosciences</a><br> | <b>Manufacturer</b>: <a href="http://www.bdbiosciences.com/eu/home" target="_blank">BD Biosciences</a><br> | ||
| <b>Model</b>: BD FACSymphony S6 SE<br> | <b>Model</b>: BD FACSymphony S6 SE<br> | ||
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| The BD FACSymphony™ S6 SE allows for high-parameter spectral flow cytometry. It enables you to gain full visible spectrum coverage to collect all light emitted without the need for filter changes, increasing dye flexibility and number of simultaneous colors. It also allows for autofluorescence unmixing, that may enable the resolution of difficult cells and populations. | The BD FACSymphony™ S6 SE allows for high-parameter spectral flow cytometry. It enables you to gain full visible spectrum coverage to collect all light emitted without the need for filter changes, increasing dye flexibility and number of simultaneous colors. It also allows for autofluorescence unmixing, that may enable the resolution of difficult cells and populations. | ||
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| * Sorting for 6-, 12-, 24-, 96-, 384-well, PCR and other custom plates | * Sorting for 6-, 12-, 24-, 96-, 384-well, PCR and other custom plates | ||
| - | **If your samples are not potentially hazardous (e.g human samples and other BSL-2 level samples) you may also sort in the [[bd_facsaria_iii |BD FACS Aria III]]**\\ | + | It shares the same optical system as the [[bd_facs_symphony_a5_se|BD FACSymphony A5 SE]] in <html><span style="color:blueviolet;font-size:100%;">Lisbon site</span></html>, allowing for the easy transfer of experiments from the analyzer to the cell sorter without the need to modify your panels. |
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| + | If you need high parameter cell sorting, check also the [[cytek_aurora_cs|CYTEK Aurora CS]] in <html><span style="color:Yellowgreen;font-size:100%;">Oeiras site</span></html>. | ||
| ===== Configuration ===== | ===== Configuration ===== | ||
| - | {{:facsymphony_optical_configuration.png?900|}} | + | <html><img src="/facility/flowcytometry/lib/exe/fetch.php?media=facsymphony_optical_configuration.png"WIDTH=900></html> |
| \\ | \\ | ||
| \\ | \\ | ||
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| ===== Suggested Fluorochrome Peak Channels ===== | ===== Suggested Fluorochrome Peak Channels ===== | ||
| - | {{{{:facsymphony_fluorophores.png|}} | + | <html><img src="/facility/flowcytometry/lib/exe/fetch.php?media=facsymphony_fluorophores.png"><br><img src="/facility/flowcytometry/lib/exe/fetch.php?media=facsymphony_fluorophores_2.png"></html> |
| - | {{{{:facsymphony_fluorophores_2.png|}} | + | |
| ===== Useful Links when Preparing your Experiment ===== | ===== Useful Links when Preparing your Experiment ===== | ||
| - | * [[prepare_samples|{{how_to_prepare_samples_for_flow_cytometry.png}}]] | + | * <html><a href="https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.ucf.005_-_how_to_prepare_samples_for_flow_cytometry.pdf" target="_blank"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=how_to_prepare_samples_for_flow_cytometry.png" WIDTH=400></a></html> |
| * <html><a href="http://www.bdbiosciences.com/en-us/applications/research-applications/multicolor-flow-cytometry/product-selection-tools/spectrum-viewer" target="_blank"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=bd_spectrum_viewer.png" WIDTH=300></a></html> | * <html><a href="http://www.bdbiosciences.com/en-us/applications/research-applications/multicolor-flow-cytometry/product-selection-tools/spectrum-viewer" target="_blank"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=bd_spectrum_viewer.png" WIDTH=300></a></html> | ||
| - | * <html><a href="http://www.bdbiosciences.com/en-us/applications/research-applications/multicolor-flow-cytometry/product-selection-tools/spectrum-viewer" target="_blank"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=bd_research_cloud.png" WIDTH=300></a></html> | + | * <html><a href="https://www.bdbiosciences.com/en-us/products/software/bd-research-cloud" target="_blank"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=bd_research_cloud.png" WIDTH=300></a></html> |
| ===== Booking Rules ===== | ===== Booking Rules ===== | ||
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| The cell sorting service is provided by a dedicated technician upon reservation on the online booking system. | The cell sorting service is provided by a dedicated technician upon reservation on the online booking system. | ||
| - | The system can be booked up to 2 months in advance. | + | The system can be booked up to 1 months in advance. |
| - | Please check the {{ ::sop.ucf.004_-_ucf_imm_user_guidelines.pdf |User Guidelines}} for detailed information. | + | Please check the <html><a href="https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.ucf.004_-_ucf_imm_user_guidelines_.pdf" target="_blank"> User Guidelines</a></html> for detailed information. |
| ---- | ---- | ||
| [[resources|Back to the Equipment page]] | [[resources|Back to the Equipment page]] | ||
bd_facsymphony_s6_se.1724342954.txt.gz · Last modified: 2024/08/22 18:09 by flowcytometry
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