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bd_facsdiscover_s8 [2025/01/06 18:30] flowcytometry |
bd_facsdiscover_s8 [2025/12/23 17:21] (current) flowcytometry |
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| - | <img src="/facility/flowcytometry/lib/exe/fetch.php?media=s8_name_wiki.png"> | + | <img src="/facility/flowcytometry/lib/exe/fetch.php?media=s8_name_wiki.png" WIDTH=680> |
| <table style="border:0px solid white;"> | <table style="border:0px solid white;"> | ||
| <tr style="border:0px solid white;"> | <tr style="border:0px solid white;"> | ||
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| * **BD SpectralFX Technology** - combines full-spectrum optics, next gen QC and system-aware spectral unmixing, managing spread by adapting to instrument performance and sample conditions in real time | * **BD SpectralFX Technology** - combines full-spectrum optics, next gen QC and system-aware spectral unmixing, managing spread by adapting to instrument performance and sample conditions in real time | ||
| - | * **BD CellView Image Technology** - the novel high-speed imaging technology that facilitates analysis and sorting through **__real-time integration of image and flow data__** | + | * **BD CellView Image Technology** - the novel high-speed imaging technology that facilitates analysis and sorting through __real-time integration of image and flow data__ |
| {{ :imaging_features.png?400|}} | {{ :imaging_features.png?400|}} | ||
| * Combines **15 image features** with flow parameters for subset classification, statistical analysis and sorting | * Combines **15 image features** with flow parameters for subset classification, statistical analysis and sorting | ||
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| * Sorting can be performed with speeds up to 12,000 events/second | * Sorting can be performed with speeds up to 12,000 events/second | ||
| * 85 μm, 100 μm and 130 μm nozzles available | * 85 μm, 100 μm and 130 μm nozzles available | ||
| - | * Up to 6-way sorting for 85 μm nozzle | + | * Up to 6-way sorting with 85 μm nozzle |
| - | * Up to 4-way sorting for 100 μm nozzle | + | * Up to 4-way sorting with 100 μm nozzle |
| - | * Up to 2-way sorting for 130 μm nozzle | + | * Up to 2-way sorting with 130 μm nozzle |
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| - | * Tube sorting for 1.5 mL eppendorfs and 5 mL tubes (FACS tubes) | + | * Up to 6-way sorting into 1.5 mL, 2 mL eppendorfs and 5 mL tubes (FACS tubes) |
| + | * Up to 4-way sorting into mixed tube formats, including 15 mL and 50 mL tubes. | ||
| * Plate sorting for 6-, 24-, 96-, 384-well, microscope slides and other custom plates | * Plate sorting for 6-, 24-, 96-, 384-well, microscope slides and other custom plates | ||
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| The cell sorting service is provided by a dedicated technician upon reservation on the online booking system. | The cell sorting service is provided by a dedicated technician upon reservation on the online booking system. | ||
| - | The system can be booked up to 1 months in advance. | + | The system can be booked up to 1 month in advance. |
| Please check the <html><a href="https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.ucf.004_-_ucf_imm_user_guidelines_.pdf" target="_blank"> User Guidelines</a></html> for detailed information. | Please check the <html><a href="https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.ucf.004_-_ucf_imm_user_guidelines_.pdf" target="_blank"> User Guidelines</a></html> for detailed information. | ||