bd_facsdiscover_s8
Differences
This shows you the differences between two versions of the page.
| Both sides previous revision Previous revision Next revision | Previous revision | ||
|
bd_facsdiscover_s8 [2025/01/06 18:30] flowcytometry |
bd_facsdiscover_s8 [2025/01/20 23:02] (current) flowcytometry [Booking Rules] |
||
|---|---|---|---|
| Line 32: | Line 32: | ||
| ===== System overview ===== | ===== System overview ===== | ||
| - | The BD FACS Discover S8 is the first cell sorter that **__integrates imaging and full-spectrum capabilities__**. This versatile sorter offers both traditional sorting using standard fluorescent parameters, typical of conventional/full-spectrum sorters, and advanced imaging features, which can be visualized through histograms and bivariate plots. This significant innovation allows cells to be sorted not only based on the proteins they express but also by the spatial distribution of those proteins within the cell. | + | The BD FACS Discover S8 is the first cell sorter that **__integrates imaging and full-spectrum capabilities__**. This versatile sorter offers both traditional sorting using standard fluorescent parameters, typical of conventional full-spectrum sorters, and advanced imaging features, which can be visualized through histograms and bivariate plots. This significant innovation allows cells to be sorted not only based on the proteins they express but also by the spatial distribution of those proteins within the cell. |
| {{ :new_insights.png?500|}} | {{ :new_insights.png?500|}} | ||
| Line 46: | Line 46: | ||
| * **BD SpectralFX Technology** - combines full-spectrum optics, next gen QC and system-aware spectral unmixing, managing spread by adapting to instrument performance and sample conditions in real time | * **BD SpectralFX Technology** - combines full-spectrum optics, next gen QC and system-aware spectral unmixing, managing spread by adapting to instrument performance and sample conditions in real time | ||
| - | * **BD CellView Image Technology** - the novel high-speed imaging technology that facilitates analysis and sorting through **__real-time integration of image and flow data__** | + | * **BD CellView Image Technology** - the novel high-speed imaging technology that facilitates analysis and sorting through __real-time integration of image and flow data__ |
| {{ :imaging_features.png?400|}} | {{ :imaging_features.png?400|}} | ||
| * Combines **15 image features** with flow parameters for subset classification, statistical analysis and sorting | * Combines **15 image features** with flow parameters for subset classification, statistical analysis and sorting | ||
| Line 54: | Line 54: | ||
| * Sorting can be performed with speeds up to 12,000 events/second | * Sorting can be performed with speeds up to 12,000 events/second | ||
| * 85 μm, 100 μm and 130 μm nozzles available | * 85 μm, 100 μm and 130 μm nozzles available | ||
| - | * Up to 6-way sorting for 85 μm nozzle | + | * Up to 6-way sorting with 85 μm nozzle |
| - | * Up to 4-way sorting for 100 μm nozzle | + | * Up to 4-way sorting with 100 μm nozzle |
| - | * Up to 2-way sorting for 130 μm nozzle | + | * Up to 2-way sorting with 130 μm nozzle |
| | | ||
| * Tube sorting for 1.5 mL eppendorfs and 5 mL tubes (FACS tubes) | * Tube sorting for 1.5 mL eppendorfs and 5 mL tubes (FACS tubes) | ||
| Line 78: | Line 78: | ||
| The cell sorting service is provided by a dedicated technician upon reservation on the online booking system. | The cell sorting service is provided by a dedicated technician upon reservation on the online booking system. | ||
| - | The system can be booked up to 1 months in advance. | + | The system can be booked up to 1 month in advance. |
| Please check the <html><a href="https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.ucf.004_-_ucf_imm_user_guidelines_.pdf" target="_blank"> User Guidelines</a></html> for detailed information. | Please check the <html><a href="https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.ucf.004_-_ucf_imm_user_guidelines_.pdf" target="_blank"> User Guidelines</a></html> for detailed information. | ||
bd_facsdiscover_s8.1736184617.txt.gz · Last modified: 2025/01/06 18:30 by flowcytometry
Page Tools
