bd_facsaria_iii
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bd_facsaria_iii [2024/08/23 16:11] flowcytometry |
bd_facsaria_iii [2025/12/15 11:52] (current) flowcytometry |
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| <img src="/facility/flowcytometry/lib/exe/fetch.php?media=aria_iii_wiki_new.png"WIDTH=300> | <img src="/facility/flowcytometry/lib/exe/fetch.php?media=aria_iii_wiki_new.png"WIDTH=300> | ||
| <td style="border:0px solid white;"> <p style="line-height:1.8"> | <td style="border:0px solid white;"> <p style="line-height:1.8"> | ||
| - | <b>Location</b>: Lisbon site: Room P2-A-49</a> (<img src="/facility/flowcytometry/lib/exe/fetch.php?media=telephone_icon.gif" width=15> 47224) <br> | + | <b>Location</b>: Lisbon Site, Room P2-A-49</a> (<img src="/facility/flowcytometry/lib/exe/fetch.php?media=telephone_icon.gif" width=15> 47224) <br> |
| <b>Manufacturer</b>: <a href="http://www.bdbiosciences.com/eu/home" target="_blank">BD Biosciences</a><br> | <b>Manufacturer</b>: <a href="http://www.bdbiosciences.com/eu/home" target="_blank">BD Biosciences</a><br> | ||
| - | <b>Model</b>: <a href="http://www.bdbiosciences.com/us/instruments/research/cell-sorters/bd-facsaria-iii/m/744763/overview" target="_blank">FACSAria III</a><br> | + | <b>Model</b>: <a href="https://www.bdbiosciences.com/en-us/products/instruments/flow-cytometers/research-cell-sorters/bd-facsaria-iii" target="_blank">FACSAria III</a><br> |
| - | <b>Nickname</b>: "Aria 3"<br> | + | <b>Nickname</b>: "Aria III"<br> |
| - | <b>Software</b>: FACSDiva 6.1.3<br> | + | <b>Software</b>: BD FACSDiva 6.1.3<br> |
| <b>Year</b>: 2011 <br> | <b>Year</b>: 2011 <br> | ||
| <b>SN</b>: P28200134 <br> | <b>SN</b>: P28200134 <br> | ||
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| </table> | </table> | ||
| </html> | </html> | ||
| - | {{:info.gif|}} **Next Scheduled Maintenances**: 1 April and 23 September | + | {{:info.gif|}} **Next Scheduled Maintenances**: 31 March and 15 September |
| {{:info.gif|}} [[aria_iii_contamination|Pseudomonas contamination timeline]] | {{:info.gif|}} [[aria_iii_contamination|Pseudomonas contamination timeline]] | ||
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| * Multicolor analysis of up to 16 parameters | * Multicolor analysis of up to 16 parameters | ||
| * 70 μm, 85 μm, 100 μm and 130 μm nozzles available | * 70 μm, 85 μm, 100 μm and 130 μm nozzles available | ||
| - | * Up to 4-way sorting into 1.5 ml or 4 ml tubes and 2-way sorting into 15 ml tubes | + | * Up to 4-way sorting into 1.5 ml, 2 mL eppendorfs and 5 mL tubes (FACS tubes) |
| + | * 2-way sorting into 15 ml tubes | ||
| * Sorting for 6-, 12-, 24-, 96-, 384-well and PCR plates | * Sorting for 6-, 12-, 24-, 96-, 384-well and PCR plates | ||
| - | **If you need to sort potentially hazardous samples (e.g human samples and other BSL-2 level samples) you need to sort in the [[bd_facsaria_fusion|BD FACSAria Fusion]] or [[bd_facsymphony_s6_se|BD FACSymphony S6 SE]] equiped with a Class II Type A2 biosafety cabinet to reduce the risk of operator exposure.** | + | It shares the same optical system as the [[bd_lsr_fortessa_x20|BD LSRFortessa X20]] in <html><span style="color:blueviolet;font-size:100%;">Lisbon site</span></html> and the [[bd_lsr_fortessa_x20_oeiras|BD LSRFortessa X20]] in <html><span style="color:yellowgreen;font-size:100%;">Oeiras site</span></html>, allowing for the easy transfer of experiments from the analyzers (BD Accuri C6 Plus, BD LSRFortessa and BD LSRFortessa X-20) to the cell sorter without the need to modify your panels. |
| - | \\ | + | __If you need to sort potentially hazardous samples (e.g human samples and other BSL-2 level samples) you need to sort in our other sorters equiped with Class II Type A2 biosafety cabinets to reduce the risk of operator exposure.__ |
| - | **If you need high parameter cell sorting, check the [[bd_facsymphony_s6_se|BD FACSymphony S6 SE]].** | + | |
| + | If you need high parameter cell sorting, check the [[bd_facsymphony_s6_se|BD FACSymphony S6 SE]] in <html><span style="color:blueviolet;font-size:100%;">Lisbon site</span></html> or the [[cytek_aurora_cs|CYTEK Aurora CS]] in <html><span style="color:Yellowgreen;font-size:100%;">Oeiras site</span></html>. | ||
| ===== Configuration ===== | ===== Configuration ===== | ||
| - | {{:bd_facsaria_iii_configuration_new_.png?900|}} | + | <html><img src="/facility/flowcytometry/lib/exe/fetch.php?media=bd_facsaria_iii_configuration_new_.png"WIDTH=900></html> |
| ===== Fluorophores Compatibility ===== | ===== Fluorophores Compatibility ===== | ||
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| <html><img src="/facility/flowcytometry/lib/exe/fetch.php?media=facsaria_iii_fluorochrome_compatibility.png"WIDTH=900><br><br> | <html><img src="/facility/flowcytometry/lib/exe/fetch.php?media=facsaria_iii_fluorochrome_compatibility.png"WIDTH=900><br><br> | ||
| <img src="/facility/flowcytometry/lib/exe/fetch.php?media=aria_additional_filters.png"WIDTH=300><br><br> | <img src="/facility/flowcytometry/lib/exe/fetch.php?media=aria_additional_filters.png"WIDTH=300><br><br> | ||
| - | <img src="/facility/flowcytometry/lib/exe/fetch.php?media=bd_lsrfortessa_x-20_405.png"WIDTH=700><br> | + | <img src="/facility/flowcytometry/lib/exe/fetch.php?media=fortessa_x20_configuration_layouts.png"><br> |
| - | <img src="/facility/flowcytometry/lib/exe/fetch.php?media=bd_facsaria_iii_488.png"WIDTH=700><br> | + | |
| - | <img src="/facility/flowcytometry/lib/exe/fetch.php?media=bd_facsaria_iii_561.png"WIDTH=700><br> | + | |
| - | <img src="/facility/flowcytometry/lib/exe/fetch.php?media=bd_facsaria_iii_633.png"WIDTH=700> | + | |
| </html> | </html> | ||
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| * <html><a href="https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.ucf.005_-_how_to_prepare_samples_for_flow_cytometry.pdf" target="_blank"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=how_to_prepare_samples_for_flow_cytometry.png" WIDTH=400></a></html> | * <html><a href="https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.ucf.005_-_how_to_prepare_samples_for_flow_cytometry.pdf" target="_blank"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=how_to_prepare_samples_for_flow_cytometry.png" WIDTH=400></a></html> | ||
| * <html><a href="http://www.bdbiosciences.com/en-us/applications/research-applications/multicolor-flow-cytometry/product-selection-tools/spectrum-viewer" target="_blank"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=bd_spectrum_viewer.png" WIDTH=300></a></html> | * <html><a href="http://www.bdbiosciences.com/en-us/applications/research-applications/multicolor-flow-cytometry/product-selection-tools/spectrum-viewer" target="_blank"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=bd_spectrum_viewer.png" WIDTH=300></a></html> | ||
| - | * <html><a href="http://www.bdbiosciences.com/en-us/applications/research-applications/multicolor-flow-cytometry/product-selection-tools/spectrum-viewer" target="_blank"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=bd_research_cloud.png" WIDTH=300></a></html> | + | * <html><a href="https://www.bdbiosciences.com/en-us/products/software/bd-research-cloud" target="_blank"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=bd_research_cloud.png" WIDTH=300></a></html> |
| ===== Booking Rules ===== | ===== Booking Rules ===== | ||
| - | The system is available for booking from 10am to 6pm from Monday to Friday. | + | The system is available for booking from 10am to 6pm from Monday to Friday. The cell sorting service is provided by a dedicated technician upon reservation on the online booking system. |
| + | |||
| + | The system is also available for booking for Power Users. | ||
| - | The cell sorting service is provided by a dedicated technician upon reservation on the online booking system. | + | The system can be booked up to 1 months in advance. |
| - | The system can be booked up to 2 months in advance. | + | |
| - | Please check the {{ ::sop.ucf.004_-_ucf_imm_user_guidelines.pdf |User Guidelines}} for detailed information. | + | Please check the <html><a href="https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.ucf.004_-_ucf_imm_user_guidelines_.pdf" target="_blank"> User Guidelines</a></html> for detailed information. |
| ---- | ---- | ||
| [[resources|Back to the Equipment page]] | [[resources|Back to the Equipment page]] | ||
bd_facsaria_iii.1724422274.txt.gz · Last modified: 2024/08/23 16:11 by flowcytometry
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