bd_facsaria_ii_oeiras
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bd_facsaria_ii_oeiras [2025/01/03 16:19] flowcytometry |
bd_facsaria_ii_oeiras [2025/11/11 13:08] (current) flowcytometry |
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| </table> | </table> | ||
| </html> | </html> | ||
| - | {{:info.gif|}} **Next Scheduled Maintenances**: | + | {{:info.gif|}} **Next Scheduled Maintenances**: 14 May and 28 November |
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| + | {{:info.gif|}} [[aria_iiu_contamination|Pseudomonas contamination timeline]] | ||
| ===== System overview ===== | ===== System overview ===== | ||
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| Sorting can be performed into two or four tubes with speeds up to 25,000 events/second. The system sorts by incorporating cells from the sample tube into a stream of sterile PBS. The stream is interrogated by the lasers at the flow cell and the system electronics keeps track of each cell as they pass through the laser and determines specific cells that meet the sort criteria. A transducer vibrates the stream and induces droplet formation, with cells in the stream being incorporated into the droplets. If a cell meets the sorting criteria and is in the last drop before the break off, the instrument will charge that drop. The charged droplet is then deflected into the proper collection tube by the charge plates. Different cell types can be sorted with the use of 70 μm, 85 μm and 100 μm nozzles. | Sorting can be performed into two or four tubes with speeds up to 25,000 events/second. The system sorts by incorporating cells from the sample tube into a stream of sterile PBS. The stream is interrogated by the lasers at the flow cell and the system electronics keeps track of each cell as they pass through the laser and determines specific cells that meet the sort criteria. A transducer vibrates the stream and induces droplet formation, with cells in the stream being incorporated into the droplets. If a cell meets the sorting criteria and is in the last drop before the break off, the instrument will charge that drop. The charged droplet is then deflected into the proper collection tube by the charge plates. Different cell types can be sorted with the use of 70 μm, 85 μm and 100 μm nozzles. | ||
| - | **The fully integrated Class II Type A2 biosafety cabinet meets personal and product protection, reducing the risk of operator exposure, while sorting potentially hazardous samples (e.g human samples and other BSL-2 level samples)**. | ||
| * Three lasers - violet (405 nm), blue (488 nm) and red (633 nm) | * Three lasers - violet (405 nm), blue (488 nm) and red (633 nm) | ||
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| * Up to 4-way sorting into 1.5 ml or 4 ml tubes and 2-way sorting into 15 ml tubes | * Up to 4-way sorting into 1.5 ml or 4 ml tubes and 2-way sorting into 15 ml tubes | ||
| * Sorting for 6-, 12-, 24-, 96-, 384-well and PCR plates | * Sorting for 6-, 12-, 24-, 96-, 384-well and PCR plates | ||
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| + | If you need to sort potentially hazardous samples (e.g human samples and other BSL-2 level samples) you need to sort in our other sorters equiped with Class II Type A2 biosafety cabinets to reduce the risk of operator exposure. | ||
| If you need a cell sorting up to 16 parameters, check the [[bd_facsaria_iii|BD FACSAria III]] and the [[bd_facsaria_fusion|BD FACSAria Fusion]] in <html><span style="color:blueviolet;font-size:100%;">Lisbon site</span></html>. | If you need a cell sorting up to 16 parameters, check the [[bd_facsaria_iii|BD FACSAria III]] and the [[bd_facsaria_fusion|BD FACSAria Fusion]] in <html><span style="color:blueviolet;font-size:100%;">Lisbon site</span></html>. | ||
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| <html><img src="/facility/flowcytometry/lib/exe/fetch.php?media=bd_facsaria_iiu_oeiras_fluorophores_wiki_new.png"><br><br> | <html><img src="/facility/flowcytometry/lib/exe/fetch.php?media=bd_facsaria_iiu_oeiras_fluorophores_wiki_new.png"><br><br> | ||
| - | <img src="/facility/flowcytometry/lib/exe/fetch.php?media=additional_filters_aria_iiu.png"><br><br> | + | <img src="/facility/flowcytometry/lib/exe/fetch.php?media=additional_filters_aria_iiu.png"width=280><br><br> |
| - | <img src="/facility/flowcytometry/lib/exe/fetch.php?media=bd_facsaria_iiu_oeiras_405_new.png"WIDTH=700><br> | + | <img src="/facility/flowcytometry/lib/exe/fetch.php?media=aria_iiu_configuration_layout.png"><br> |
| - | <img src="/facility/flowcytometry/lib/exe/fetch.php?media=bd_facsaria_iiu_488.png"WIDTH=700><br> | + | |
| - | <img src="/facility/flowcytometry/lib/exe/fetch.php?media=bd_facsaria_iiu_633.png"WIDTH=700> | + | |
| </html> | </html> | ||
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| ===== Booking Rules ===== | ===== Booking Rules ===== | ||
| - | The system is available for booking from 10am to 5pm from Monday to Friday. | + | The system is available for booking from 10am to 5pm from Monday to Friday. The cell sorting service is provided by a dedicated technician upon reservation on the online booking system. |
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| + | The system is also available for booking for Power Users. | ||
| - | The cell sorting service is provided by a dedicated technician upon reservation on the online booking system. | ||
| The system can be booked up to 1 month in advance. | The system can be booked up to 1 month in advance. | ||
bd_facsaria_ii_oeiras.1735917553.txt.gz · Last modified: 2025/01/03 16:19 by flowcytometry
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