about
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about [2019/07/15 17:27] flowcytometry [Team] |
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| - | ====== About the Flow Cytometry Facility ====== | + | ====== The Flow Cytometry Platform ====== |
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| + | The Flow Cytometry Platform provides a state of the art flow cytometry service and expertise to GIMM researchers as well as external groups from other research institutes and companies.\\ | ||
| - | The Flow Cytometry Facility provides a state of the art flow cytometry service to iMM Lisboa researchers as well as external groups from other research institutes. We aim at training researchers in the adequate use of the instruments and also on flow cytometry principles, experiment planning, as well as data analysis and interpretation. The Flow Cytometry Facility also provides a cell sorting service using two High Speed Cell Sorters (BD FACSAria III and BD FACSAria IIu) operated by dedicated staff. | + | Platform staff is well trained in instrument operation, providing support to experimental planning, sample acquisition, panel design and data analysis. Training is available for all analytical instruments and SOP are implemented to comply with the highest quality standards required to ensure reproducibility in science.\\ |
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| - | ===== Team ===== | + | If you want to purify a cell population in your sample, we can help you by providing a cell sorting service using 5 high speed cell sorters (BD FACSAria IIu, BD FACSAria Fusion, BD FACSAria III and two BD FACSDiscover S8).\\ |
| + | We’re here to help with your flow cytometry experiments. Get in touch and we’ll give you a guided tour of our facility so you can get the most out of your work with us! | ||
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| - | <table style="border:0px solid white;"> | + | <div style="text-align:center;"> |
| - | <td style="border:0px solid white;"> | + | <img src="/facility/flowcytometry/lib/exe/fetch.php?media=services_new.png" |
| - | <div align="center"><b>Head of Facility</b></div> | + | style="max-width:100%; height:auto;"> |
| - | <br> | + | </div> |
| - | <div style="width: 150px; height: 150px; overflow: hidden; position: relative;"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=jose_rino.png" style="width:150px; height: 150px;" /></div><br><br> | + | |
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| - | <b>José Rino</b> has a <i>Licenciatura</i> in Technological Physics Engineering (Instituto Superior Técnico) and a PhD in Biophysics (Faculdade de Ciências). José was appointed Head of the Flow Cytometry Facility in October 2016. He is also Head of the Bioimaging Facility at iMM. <a href="https://imm.medicina.ulisboa.pt/facility/bioimaging/doku.php?id=facility_bioimaging_doku.php&id=rino_cv">More information</a>.<br> | + | |
| - | <img src="https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=mail_icon.png" width=20> | + | |
| - | <i>joserino@medicina.ulisboa.pt</i> | + | |
| - | </td> | + | |
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| + | \\ | ||
| + | ===== How to use the service ===== | ||
| - | <html> | + | * The Flow Cytometry Platform systems are available to GIMM researchers and external users. |
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| - | <div align="center"><b>Technical Support</b></div> | + | |
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| - | <div style="width: 150px; height: 150px; overflow: hidden; position: relative;"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=andreia_santos.png" style="width:150px; height: 150px;" /></div><br><br> | + | |
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| - | <b>Andreia Santos</b> has a BSc in Biology and a MSc in Genetic and Molecular Biology from Faculdade de Ciências, Universidade de Lisboa. Andreia joined the Flow Cytometry Facility in September 2017 after working at Ana Espada de Sousa’s lab on human T-cell development, namely the role of IL-4 in the lineage plasticity of human CD4 single positive thymocytes.<br> | + | |
| - | <img src="https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=mail_icon.png" width=20> | + | |
| - | <i>assantos@medicina.ulisboa.pt</i> | + | |
| - | </td> | + | |
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| - | <div style="width: 150px; height: 150px; overflow: hidden; position: relative;"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=rute_goncalves.png" style="width:150px; height: 150px;" /></div><br><br> | + | |
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| - | <b>Rute Gonçalves</b> has a BSc in Biochemistry and a MSc in Molecular Genetics and Biomedicine from Universidade Nova de Lisboa. Rute joined the Flow Cytometry Facility in December 2018, after working at the Immunobiology and Pathogenesis Lab at CEDOC, under the supervision of Dra. Helena Soares, studying the main TLR4 signaling pathways in human T cells. She was also responsible for CEDOC’s Flow Cytometry Facility from April 2018 to October 2018, providing cell sorting services, supporting researchers in cell analyzers as well as software and data analysis and of course performing all the daily equipment maintenance and quality control. <br> | + | |
| - | <img src="https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=mail_icon.png" width=20> | + | |
| - | <i>rute.goncalves@medicina.ulisboa.pt</i> | + | |
| - | </td> | + | |
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| - | <div style="width: 150px; height: 150px; overflow: hidden; position: relative;"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=joana_monteiro.png" style="width:150px; height: 150px;" /></div><br><br> | + | |
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| - | <b>Joana Monteiro</b> has a <i>Licenciatura</i> in Biochemistry from the Faculdade de Ciências, Universidade de Lisboa. Joana joined the Flow Cytometry Facility in June 2019, after working at the startup Lumisense and before that studying stem cells and metastasis in the Erasmus Medical Center Rotterdam, in the group of Prof. Riccardo Fodde. Joana has already attended a BD Course for basic operator of the FACSAria.<br> | + | |
| - | <img src="https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=mail_icon.png" width=20> | + | |
| - | <i>joana.monteiro@medicina.ulisboa.pt</i> | + | |
| - | </td> | + | |
| - | </tr> | + | |
| - | </table> | + | |
| - | </html> | + | |
| - | ===== Services ===== | + | * [[supportservices|Support Services]], such as **Consulting** (1st Meeting Project Presentation, Experimental Planning, Sample Preparation, and Troubleshooting), **Panel Design** and **Data Analysis** are available. Book a meeting with our team to discuss your experiments and how we can support you. |
| - | Our flow cytometers are grouped into two categories: cell analyzers and cell sorters. Cell Analyzers are instruments for acquisition only, cells cannot be recovered once they are analyzed in the instrument. Cell sorters are capable of physically “sorting” out a population(s) of interest. | + | * If you need to use our analyzers frequently, follow the [[newuser|Training guidelines]] to have **Training** in our systems. Depending on your needs and usage frequency, you may acquire data with our assistance. We recommend booking a meeting with our team to discuss your needs and options. |
| - | {{red-arrow-pointing-right.jpg|}} **More info on our** [[resources|{{flow_resources.jpg?0x30|}}]] | + | * If you need to use our **Cell Sorters**, follow the [[ucfservicesorting|Cell Sorting guidelines]] to have access to our systems. |
| + | \\ | ||
| + | ===== Equipment ===== | ||
| - | {{::ucf_process.jpg|}} | + | Check our [[resources|Cell Analysers and Cell Sorters]] and [[workstations|Workstations]]. |
| - | ===== Prices ===== | + | \\ |
| + | ===== Policies and Charges ===== | ||
| - | Users are billed for the actual time of usage. [[https://imm.medicina.ulisboa.pt/intranet/booking/select_resource.php?v=1|Online booking]] is mandatory for each usage session. **Cancellations and no shows will be charged extra**, please check the [[usage_policy|usage rules]] for more details. | + | <html><a href="https://gimm.agendoscience.com/" target="_blank">Online booking</a></html> is mandatory for each usage session. |
| + | \\ | ||
| - | {{red-arrow-pointing-right.jpg|}} **Check our** [[billing|{{charge_rates.jpg?0x30|}}]] | + | **Check our** [[billing|Usage Rules and Charge Policies]]. |
| - | ===== Hours ===== | + | \\ |
| + | ===== Platform Availability ===== | ||
| - | **9 a.m. - 6 p.m.** for **Training, Assistance and Consultations** | + | **24 hours, 7 days a week** for Equipment Usage by __authorized and trained users__.\\ |
| + | **10 a.m. – 6 p.m.** for **Cell Sorting**.\\ | ||
| + | **10 a.m. – 12 p.m.** and **2 p.m. – 5 p.m.** for **Training, Assistance and Consultations**.\\ | ||
| - | 24 hours, 7 days a week for Equipment Usage by **authorized, trained users** (see [[usage_policy|Usage Rules]]) | + | **Internal meetings**: The platform team is __unavailable__ on **Mondays, 11 a.m. – 12 p.m.**, and **Fridays, 10 a.m. – 1 p.m.**. These meetings allow the team to review equipment status, coordinate ongoing activities and projects, share updates and challenges, and collectively develop new ideas and strategies to optimize our workflows and services. |
| - | ===== How to use the service ===== | + | \\ |
| + | ===== Contacts ===== | ||
| - | All new users must contact the Flow Cytometry Facility to get proper training in using the systems and know the usage rules. Only after training will a user gain access to the Flow Cytometry room and to the online booking system. To get access to the cell sorters online booking, users must contact the Flow Cytometry staff. | + | <html> |
| - | Please read our policies and guidelines [[https://imm.medicina.ulisboa.pt/files/6714/6348/2676/SOP.UCF.004_-_UCF_IMM_USER_GUIDELINES.pdf|here]]. | + | |
| - | {{:UCF_users_over_years.png?400|}} {{:UCF_usage_hours.png?400|}} \\ | + | <div style="margin-bottom:20px;"> |
| + | <div style="font-style:italic; font-size:15px; font-weight:400;">Flow Cytometry Platform</div> | ||
| + | <div style="margin-top:4px;"> | ||
| + | <a href="mailto:flowcytometry@gimm.pt">flowcytometry@gimm.pt</a> | ||
| + | </div> | ||
| + | </div> | ||
| - | {{red-arrow-pointing-right.jpg|}} **Are you a** [[newuser|{{new_user.jpg?0x30|}}]] | + | <div style="display:flex; gap:12px; width:650px;"> |
| - | ===== Contacts ===== | + | <div style="flex:1; padding:12px 18px 10px; background:#fafafa; border:1px solid #e1e5e9; border-left:6px solid #7b4bb7; border-radius:4px;"> |
| - | Flow Cytometry Facility\\ | + | <div style="font-size:16px; font-weight:600; color:#7b4bb7; margin-bottom:10px;"> |
| - | **imm-flowcytometry@medicina.ulisboa.pt** | + | Lisbon site |
| + | </div> | ||
| - | Instituto de Medicina Molecular, room P2-A-49\\ | + | <div style="font-size:13px; padding:5px 0; border-top:1px solid #e9ecef; line-height:1.5;"> |
| - | Faculdade de Medicina de Lisboa \\ | + | Faculdade de Medicina de Lisboa<br>Room P2-A-49 & P2-A-32<br>Av. Prof. Egas Moniz<br>1649-028 Lisboa |
| - | Av. Prof. Egas Moniz \\ | + | </div> |
| - | 1649-028 Lisboa \\ | + | |
| + | <div style="font-size:13px; padding:5px 0; border-top:1px solid #e9ecef; line-height:1.2;"> | ||
| + | <span style="color:#7a8491;">Internal</span><br> | ||
| + | 47222 / 47224 | ||
| + | </div> | ||
| - | {{flow_cytometry_map.jpg?700|}} | + | <div style="font-size:13px; padding:5px 0 1px; border-top:1px solid #e9ecef; line-height:1.2;"> |
| - | + | <span style="color:#7a8491;">External</span><br> | |
| - | {{telephone_icon.gif?20|}}Internal phone extension: 47222 / 47224 | + | <a href="tel:+351935002815">+351 935 002 815</a> |
| + | </div> | ||
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| + | </div> | ||
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| + | <div style="flex:1; padding:12px 18px 10px; background:#fafafa; border:1px solid #e1e5e9; border-left:6px solid #7aaa28; border-radius:4px;"> | ||
| + | |||
| + | <div style="font-size:16px; font-weight:600; color:#7aaa28; margin-bottom:10px;"> | ||
| + | Oeiras site | ||
| + | </div> | ||
| + | |||
| + | <div style="font-size:13px; padding:5px 0; border-top:1px solid #e9ecef; line-height:1.5;"> | ||
| + | R. Q.ta Grande 6<br>Room 0B01 & 0B14<br>Bartolomeu Dias Wing<br>2780-156 Oeiras | ||
| + | </div> | ||
| + | |||
| + | <div style="font-size:13px; padding:5px 0; border-top:1px solid #e9ecef; line-height:1.2;"> | ||
| + | <span style="color:#7a8491;">Internal</span><br> | ||
| + | 4625 | ||
| + | </div> | ||
| + | |||
| + | <div style="font-size:13px; padding:5px 0 1px; border-top:1px solid #e9ecef; line-height:1.2;"> | ||
| + | <span style="color:#7a8491;">External</span><br> | ||
| + | <a href="tel:+351935002594">+351 935 002 594</a> | ||
| + | </div> | ||
| + | |||
| + | </div> | ||
| + | |||
| + | </div> | ||
| + | |||
| + | </html> | ||
| ---- | ---- | ||
| [[start|Back to the Flow Cytometry Wiki]] | [[start|Back to the Flow Cytometry Wiki]] | ||
about.1563204475.txt.gz · Last modified: 2019/07/15 17:27 by flowcytometry
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