usage_policy
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usage_policy [2017/01/18 16:21] 10.97.105.213 |
usage_policy [2024/02/28 22:35] (current) flowcytometry [Cell Sorters] |
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| - | <html><img src="/facility/flowcytometry/lib/exe/fetch.php?media=usage_rules.jpg"><BR><BR></html> | + | <html><img src="/facility/flowcytometry/lib/exe/fetch.php?media=usage_rules_v2_1.png"><BR><BR></html> |
| - | Please download the detailed [[https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.ucf.004_-_ucf_imm_user_guidelines.pdf|User Guidelines]] for your own reference. | + | Please download the detailed <html><a href="https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.ucf.004_-_ucf_imm_user_guidelines.pdf" target="_blank"> User Guidelines </a></html> for your own reference. |
| ===== Cell Analyzers ===== | ===== Cell Analyzers ===== | ||
| - | - Users must get trained on a cell analyzer before using it. Use the booking system or [[imm-flowcytometry@medicina.ulisboa.pt|email us]] to request training. | + | - Users must get trained on a cell analyzer before using it. Use the <html><a href="https://imm.agendoscience.com" target="_blank"> booking system </a></html> or [[imm-flowcytometry@medicina.ulisboa.pt|email us]] to request training. |
| - | - After training, users must [[imm-flowcytometry@medicina.ulisboa.pt|email us]] their full name, Lab and phone extension to get room access permission | + | - The cell analyzers **can be booked up to 2 months in advance**. |
| - | - The cell analyzers **can be booked up to 2 months in advance** | + | - Users must **include 15 min in their booking time for cleaning**. |
| - | - Users must have a valid reservation in an imaging system before using it. | + | - Users must have a valid reservation in a system before using it. |
| - Users must use their own booked time and not book or use a resource with other username. | - Users must use their own booked time and not book or use a resource with other username. | ||
| - | - Users can book unlimited slots but **can only cancel 3h before the slot starting time** | + | - Users **can only cancel bookings 3h before the slot starting time**. |
| - | - At the end of an acquisition, users must contact the next person that is booked | + | - Users must record start and finish times on the system log sheet (cleaning time included) and problems (if any). |
| - | - Users must record start and finish times on the system log sheet (cleaning time included) and problems (if any) | + | - Users that swap filters must leave the system with the default configuration. |
| - | - Users that swap filters must leave the system with the default configuration | + | - Users must log out from the analyzer software at the end of their session. |
| - | - Users must log off from the analyzer software at the end of their session | + | - Users must **switch off the system** if they are the **last user booked** or if the analyzer **will not be used for at least 3h** (Fortessa 2 & Fortessa X-20). |
| - | - The last booked user of the day is responsible for switching the system off | + | - Users must always export their data and delete it from the FACSDiva/SpectroFlo database regularly. |
| - | - Users must always export their data, delete it from the database and write down the data transfer in the logsheet | + | - Users must **always clean the machines** after acquisition following the cleaning protocol and **record the cleaning steps** in FACSDiva/SpectroFlo. |
| - | - Users must **always clean the machines**, empty the waste reservoir and refill the sheath tank with PBS1X+Azide. | + | - Users must perform an additional cleaning before acquisition if they are the **first user of the day**. |
| + | - Users must **refill the sheath tank** with PBS+Azide at the end of the booked session. | ||
| + | - If during acquisition, the **waste container gets full**, a high pitch sound will go off. Users should **disconnect the black sensor** (the noise will stop), put the cytometer in **Standby**, and **disconnect | ||
| + | the orange connector.** The container must be replaced with a new one. | ||
| - | {{warning.jpg?20|}} ** Last Minute Cancellations** (within 3h before the starting booked time) must be requested by [[imm-flowcytometry@medicina.ulisboa.pt|email]] \\ | + | {{warning.jpg?20|}} Users will be **billed according to the actual starting time or booked starting time (whichever occurs first) and the actual ending time or booked ending time (whichever occurs last)** |
| - | **The penalty charge is 2x the cost/hour of booked time** \\ | + | |
| - | (penalty charges will not be applied to clinical samples or mouse samples requiring "same-day" genotyping, but booked time will be charged) | + | |
| - | {{warning.jpg?20|}} ** NO SHOWS** - **the charge is 3x the cost/hour** | + | {{warning.jpg?20|}} ** Last Minute Cancellations** (within 3h before the starting booked time) may result in a penalization \\ |
| + | **The penalty charge corresponds to the running cost of the system if the session is not booked by another user** \\ | ||
| + | |||
| + | {{warning.jpg?20|}} ** NO SHOWS** - **the charge corresponds to the running cost of the system** | ||
| ===== Cell Sorters ===== | ===== Cell Sorters ===== | ||
| - | - Users must [[imm-flowcytometry@medicina.ulisboa.pt|contact us]] to get access to the cell sorters booking. | + | - Users must [[imm-flowcytometry@medicina.ulisboa.pt|contact us]] or use the booking system to request access to the cell sorters booking. |
| - | - The cell sorters **can be booked up to 2 months in advance** between 10am and 10pm | + | - The cell sorters **can be booked up to 2 months in advance** between 10am and 6pm. |
| - | - Users must include 15 min in their booking time for purity checks and cleaning between sorts | + | - If you need to book a sorter outside the regular working hours, please contact us beforehand to check for staff availability. |
| - | - Users must mention in the booking comments which nozzle they will be using (70 μm, 85 μm or 100 μm) | + | - Users must **include time in their booking for new settings, purity checks and cleaning** (minimum 30 min). |
| - | - Users must not book on behalf of others | + | - Users must mention in the booking comments the Biosafety Level (BSL) of their samples, which nozzle (70 μm, 85 μm or 100 μm) and which collection devices (plates, 1.5 mL tubes, 5 mL FACS tubes or 15 mL Falcon tubes) they will be using. |
| - | - Users can book unlimited slots but **can only cancel 7 days in advance** | + | - Users must not book on behalf of others. |
| - | + | - Users **can only cancel bookings 7 days in advance**. | |
| - | {{warning.jpg?20|}} ** Cancellations** (within 6 days in advance) must be requested by [[imm-flowcytometry@medicina.ulisboa.pt|email]] \\ | + | |
| - | **The penalty charge is 2x the cost/hour of booked time** \\ | + | |
| - | (penalty charges will not be applied to clinical samples or mouse samples requiring "same-day" genotyping, but booked time will be charged) | + | |
| - | + | ||
| - | {{warning.jpg?20|}} ** NO SHOWS** (1 day before or on the same day) - **the charge is 4x the cost/hour** | + | |
| - | ==== Sample Preparation ==== | + | {{warning.jpg?20|}} ** Cancellations** (within 6 days in advance) may result in a penalization \\ |
| + | **The penalty charge corresponds to the running cost of the system if the session is not booked by another user** | ||
| + | \\ | ||
| - | Remember to bring: | + | {{warning.jpg?20|}} ** NO SHOWS** - **the charge corresponds to the running cost of the system** |
| - | * correct controls | + | |
| - | * filtered cells | + | |
| - | * collection tubes | + | |
| - | * media for dilution and collection | + | |
| ===== Abuse Report ===== | ===== Abuse Report ===== | ||
usage_policy.1484752912.txt.gz · Last modified: 2017/01/18 16:21 by 10.97.105.213
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