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usage_policy [2018/05/23 16:43] flowcytometry [Cell Analyzers] |
usage_policy [2020/03/26 18:26] flowcytometry |
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- | <html><img src="/facility/flowcytometry/lib/exe/fetch.php?media=usage_rules.jpg"><BR><BR></html> | + | <html><img src="/facility/flowcytometry/lib/exe/fetch.php?media=usage_rules.png"><BR><BR></html> |
Please download the detailed [[https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.ucf.004_-_ucf_imm_user_guidelines.pdf|User Guidelines]] for your own reference. | Please download the detailed [[https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.ucf.004_-_ucf_imm_user_guidelines.pdf|User Guidelines]] for your own reference. | ||
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===== Cell Analyzers ===== | ===== Cell Analyzers ===== | ||
- Users must get trained on a cell analyzer before using it. Use the booking system or [[imm-flowcytometry@medicina.ulisboa.pt|email us]] to request training. | - Users must get trained on a cell analyzer before using it. Use the booking system or [[imm-flowcytometry@medicina.ulisboa.pt|email us]] to request training. | ||
- | - The cell analyzers **can be booked up to 6 weeks in advance** | + | - The cell analyzers **can be booked up to 2 months in advance** |
- Users must **include 15 min in their booking time for cleaning** | - Users must **include 15 min in their booking time for cleaning** | ||
- Users must have a valid reservation in a system before using it. | - Users must have a valid reservation in a system before using it. | ||
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- Users that swap filters must leave the system with the default configuration | - Users that swap filters must leave the system with the default configuration | ||
- Users must log out from the analyzer software at the end of their session | - Users must log out from the analyzer software at the end of their session | ||
- | - Users must **switch off the system** if they are the **last user booked** or the analyzer **will not be used for at least 3h** | + | - Users must **switch off the system** if they are the **last user booked** or if the analyzer **will not be used for at least 3h** |
- Users must always export their data and delete it from the FACSDiva database regularly | - Users must always export their data and delete it from the FACSDiva database regularly | ||
- Users must **always clean the machines** following the cleaning protocol and **record the cleaning steps** in FACSDiva | - Users must **always clean the machines** following the cleaning protocol and **record the cleaning steps** in FACSDiva | ||
- | - Users must **check if the waste reservoir is full** (empty it if above the limit line) and **refill the sheath tank** with PBS+Azide at the end of the booked session. | + | - Users must **refill the sheath tank** with PBS+Azide at the end of the booked session. |
+ | - If during acquisition, the **waste container gets full**, a high pitch sound will go off. Users should **disconnect the black sensor** (the noise will stop), put the cytometer in **Standby**, and **disconnect | ||
+ | the orange connector.** Close the container with the red cap that is on top of it and replace the container with a new one. | ||
{{warning.jpg?20|}} Users will be **billed according to the actual starting time or booked starting time (whichever occurs first) and the actual ending time or booked ending time (whichever occurs last)** | {{warning.jpg?20|}} Users will be **billed according to the actual starting time or booked starting time (whichever occurs first) and the actual ending time or booked ending time (whichever occurs last)** | ||
- | {{warning.jpg?20|}} ** Last Minute Cancellations** (within 3h before the starting booked time) will be penalized | + | {{warning.jpg?20|}} ** Last Minute Cancellations** (within 3h before the starting booked time) may result in a penalization \\ |
**The penalty charge corresponds to the running cost of the system if the session is not booked by another user** \\ | **The penalty charge corresponds to the running cost of the system if the session is not booked by another user** \\ | ||
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===== Cell Sorters ===== | ===== Cell Sorters ===== | ||
- | - Users must [[imm-flowcytometry@medicina.ulisboa.pt|contact us]] to get access to the cell sorters booking. | + | - Users must [[imm-flowcytometry@medicina.ulisboa.pt|contact us]] or use the booking system to request access to the cell sorters booking. |
- | - The cell sorters **can be booked up to 6 weeks in advance** between 10am and 6pm | + | - The cell sorters **can be booked up to 2 months in advance** between 10am and 6pm |
+ | - If you need to book a sorter outside the regular working hours, please contact us beforehand to check for staff availability. | ||
- Users must **include time in their booking for new settings, purity checks and cleaning** (minimum 30 min) | - Users must **include time in their booking for new settings, purity checks and cleaning** (minimum 30 min) | ||
- | - Users must mention in the booking comments which nozzle (70 μm, 85 μm or 100 μm) and collection tubes (1.5 mL tubes, 5 mL FACStubes or 15 mL Falcon tubes) they will be using | + | - Users must mention in the booking comments which nozzle (70 μm, 85 μm or 100 μm) and collection tubes (1.5 mL tubes, 5 mL FACS tubes or 15 mL Falcon tubes) they will be using |
- Users must not book on behalf of others | - Users must not book on behalf of others | ||
- Users **can only cancel bookings 7 days in advance** | - Users **can only cancel bookings 7 days in advance** | ||
- | {{warning.jpg?20|}} ** Cancellations** (within 6 days in advance) must be requested by [[imm-flowcytometry@medicina.ulisboa.pt|email]] \\ | + | {{warning.jpg?20|}} ** Cancellations** (within 6 days in advance) may result in a penalization \\ |
**The penalty charge corresponds to the running cost of the system if the session is not booked by another user** | **The penalty charge corresponds to the running cost of the system if the session is not booked by another user** | ||
\\ | \\ | ||
- | {{warning.jpg?20|}} ** NO SHOWS** (1 day before or on the same day) - **the charge corresponds to the running cost of the system** | + | {{warning.jpg?20|}} ** NO SHOWS** (or cancellation in the booked day) - **the charge corresponds to the running cost of the system** |
==== Sample Preparation ==== | ==== Sample Preparation ==== |