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usage_policy [2017/04/28 17:51] flowcytometry |
usage_policy [2020/03/27 10:29] flowcytometry |
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- | <html><img src="/facility/flowcytometry/lib/exe/fetch.php?media=usage_rules.jpg"><BR><BR></html> | + | <html><img src="/facility/flowcytometry/lib/exe/fetch.php?media=usage_rules.png"><BR><BR></html> |
Please download the detailed [[https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.ucf.004_-_ucf_imm_user_guidelines.pdf|User Guidelines]] for your own reference. | Please download the detailed [[https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.ucf.004_-_ucf_imm_user_guidelines.pdf|User Guidelines]] for your own reference. | ||
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===== Cell Analyzers ===== | ===== Cell Analyzers ===== | ||
- Users must get trained on a cell analyzer before using it. Use the booking system or [[imm-flowcytometry@medicina.ulisboa.pt|email us]] to request training. | - Users must get trained on a cell analyzer before using it. Use the booking system or [[imm-flowcytometry@medicina.ulisboa.pt|email us]] to request training. | ||
- | - After training, users must [[imm-flowcytometry@medicina.ulisboa.pt|email us]] their full name, Lab and phone extension to get room access permission | + | - The cell analyzers **can be booked up to 2 months in advance** |
- | - The cell analyzers **can be booked up to 6 weeks in advance** | + | |
- Users must **include 15 min in their booking time for cleaning** | - Users must **include 15 min in their booking time for cleaning** | ||
- | - Users must have a valid reservation in an imaging system before using it. | + | - Users must have a valid reservation in a system before using it. |
- Users must use their own booked time and not book or use a resource with other username. | - Users must use their own booked time and not book or use a resource with other username. | ||
- | - Users can book unlimited slots but **can only cancel 3h before the slot starting time** | + | - Users **can only cancel bookings 3h before the slot starting time** |
- | - At the end of an acquisition, users must contact the next person that is booked | + | |
- Users must record start and finish times on the system log sheet (cleaning time included) and problems (if any) | - Users must record start and finish times on the system log sheet (cleaning time included) and problems (if any) | ||
- Users that swap filters must leave the system with the default configuration | - Users that swap filters must leave the system with the default configuration | ||
- | - Users must log off from the analyzer software at the end of their session | + | - Users must log out from the analyzer software at the end of their session |
- | - The last booked user of the day is responsible for switching the system off | + | - Users must **switch off the system** if they are the **last user booked** or if the analyzer **will not be used for at least 3h** |
- | - Users must always export their data, delete it from the database and write down the data transfer in the logsheet | + | - Users must always export their data and delete it from the FACSDiva database regularly |
- | - Users must **always clean the machines** following the cleaning protocol, **empty the waste** reservoir and **refill the sheath tank** with PBS1X+Azide. | + | - Users must **always clean the machines** following the cleaning protocol and **record the cleaning steps** in FACSDiva |
+ | - Users must **refill the sheath tank** with PBS+Azide at the end of the booked session. | ||
+ | - If during acquisition, the **waste container gets full**, a high pitch sound will go off. Users should **disconnect the black sensor** (the noise will stop), put the cytometer in **Standby**, and **disconnect | ||
+ | the orange connector.** The container must be replaced with a new one. | ||
{{warning.jpg?20|}} Users will be **billed according to the actual starting time or booked starting time (whichever occurs first) and the actual ending time or booked ending time (whichever occurs last)** | {{warning.jpg?20|}} Users will be **billed according to the actual starting time or booked starting time (whichever occurs first) and the actual ending time or booked ending time (whichever occurs last)** | ||
- | {{warning.jpg?20|}} ** Last Minute Cancellations** (within 3h before the starting booked time) must be requested by [[imm-flowcytometry@medicina.ulisboa.pt|email]] \\ | + | {{warning.jpg?20|}} ** Last Minute Cancellations** (within 3h before the starting booked time) may result in a penalization \\ |
- | **The penalty charge is 2x the cost/hour of booked time** \\ | + | **The penalty charge corresponds to the running cost of the system if the session is not booked by another user** \\ |
- | (penalty charges will not be applied to clinical samples or mouse samples requiring "same-day" genotyping, but booked time will be charged) | + | |
- | {{warning.jpg?20|}} ** NO SHOWS** - **the charge is 3x the cost/hour** | + | {{warning.jpg?20|}} ** NO SHOWS** - **the charge corresponds to the running cost of the system** |
===== Cell Sorters ===== | ===== Cell Sorters ===== | ||
- | - Users must [[imm-flowcytometry@medicina.ulisboa.pt|contact us]] to get access to the cell sorters booking. | + | - Users must [[imm-flowcytometry@medicina.ulisboa.pt|contact us]] or use the booking system to request access to the cell sorters booking. |
- | - The cell sorters **can be booked up to 6 weeks in advance** between 10am and 10pm | + | - The cell sorters **can be booked up to 2 months in advance** between 10am and 6pm |
- | - Users must **include 15 min in their booking time for purity checks and cleaning** between sorts | + | - If you need to book a sorter outside the regular working hours, please contact us beforehand to check for staff availability. |
- | - Users must mention in the booking comments which nozzle they will be using (70 μm, 85 μm or 100 μm) | + | - Users must **include time in their booking for new settings, purity checks and cleaning** (minimum 30 min) |
+ | - Users must mention in the booking comments which nozzle (70 μm, 85 μm or 100 μm) and collection tubes (1.5 mL tubes, 5 mL FACS tubes or 15 mL Falcon tubes) they will be using | ||
- Users must not book on behalf of others | - Users must not book on behalf of others | ||
- | - Users can book unlimited slots but **can only cancel 7 days in advance** | + | - Users **can only cancel bookings 7 days in advance** |
- | {{warning.jpg?20|}} Users will be **billed according to the actual starting time or booked starting time (whichever occurs first) and the actual ending time or booked ending time (whichever occurs last)** | + | {{warning.jpg?20|}} ** Cancellations** (within 6 days in advance) may result in a penalization \\ |
- | + | **The penalty charge corresponds to the running cost of the system if the session is not booked by another user** | |
- | {{warning.jpg?20|}} ** Cancellations** (within 6 days in advance) must be requested by [[imm-flowcytometry@medicina.ulisboa.pt|email]] \\ | + | \\ |
- | **The penalty charge is 2x the cost/hour of booked time** \\ | + | |
- | (penalty charges will not be applied to clinical samples or mouse samples requiring "same-day" genotyping, but booked time will be charged) | + | |
- | {{warning.jpg?20|}} ** NO SHOWS** (1 day before or on the same day) - **the charge is 4x the cost/hour** | + | {{warning.jpg?20|}} ** NO SHOWS** (or cancellation in the booked day) - **the charge corresponds to the running cost of the system** |
==== Sample Preparation ==== | ==== Sample Preparation ==== | ||
- | Remember to bring: | + | Please check **[[prepare_samples|How to prepare samples for Flow Cytometry]]**. |
- | * correct controls | + | |
- | * filtered cells | + | |
- | * collection tubes | + | |
- | * media for dilution and collection | + | |
===== Abuse Report ===== | ===== Abuse Report ===== |