This shows you the differences between two versions of the page.
Both sides previous revision Previous revision Next revision | Previous revision Next revision Both sides next revision | ||
usage_policy [2017/04/28 17:51] flowcytometry |
usage_policy [2018/03/06 18:35] flowcytometry |
||
---|---|---|---|
Line 8: | Line 8: | ||
- The cell analyzers **can be booked up to 6 weeks in advance** | - The cell analyzers **can be booked up to 6 weeks in advance** | ||
- Users must **include 15 min in their booking time for cleaning** | - Users must **include 15 min in their booking time for cleaning** | ||
- | - Users must have a valid reservation in an imaging system before using it. | + | - Users must have a valid reservation in a system before using it. |
- Users must use their own booked time and not book or use a resource with other username. | - Users must use their own booked time and not book or use a resource with other username. | ||
- Users can book unlimited slots but **can only cancel 3h before the slot starting time** | - Users can book unlimited slots but **can only cancel 3h before the slot starting time** | ||
Line 14: | Line 14: | ||
- Users must record start and finish times on the system log sheet (cleaning time included) and problems (if any) | - Users must record start and finish times on the system log sheet (cleaning time included) and problems (if any) | ||
- Users that swap filters must leave the system with the default configuration | - Users that swap filters must leave the system with the default configuration | ||
- | - Users must log off from the analyzer software at the end of their session | + | - Users must log out from the analyzer software at the end of their session |
- The last booked user of the day is responsible for switching the system off | - The last booked user of the day is responsible for switching the system off | ||
- Users must always export their data, delete it from the database and write down the data transfer in the logsheet | - Users must always export their data, delete it from the database and write down the data transfer in the logsheet | ||
- | - Users must **always clean the machines** following the cleaning protocol, **empty the waste** reservoir and **refill the sheath tank** with PBS1X+Azide. | + | - Users must **always clean the machines** following the cleaning protocol, **check if the waste reservoir is full** (empty it if above the limit line) and **check the sheath tank** (refill with PBS+Azide everytime). |
{{warning.jpg?20|}} Users will be **billed according to the actual starting time or booked starting time (whichever occurs first) and the actual ending time or booked ending time (whichever occurs last)** | {{warning.jpg?20|}} Users will be **billed according to the actual starting time or booked starting time (whichever occurs first) and the actual ending time or booked ending time (whichever occurs last)** | ||
{{warning.jpg?20|}} ** Last Minute Cancellations** (within 3h before the starting booked time) must be requested by [[imm-flowcytometry@medicina.ulisboa.pt|email]] \\ | {{warning.jpg?20|}} ** Last Minute Cancellations** (within 3h before the starting booked time) must be requested by [[imm-flowcytometry@medicina.ulisboa.pt|email]] \\ | ||
- | **The penalty charge is 2x the cost/hour of booked time** \\ | + | **The penalty charge corresponds to the running cost of the system if the session is not booked by another user** \\ |
- | (penalty charges will not be applied to clinical samples or mouse samples requiring "same-day" genotyping, but booked time will be charged) | + | |
- | {{warning.jpg?20|}} ** NO SHOWS** - **the charge is 3x the cost/hour** | + | {{warning.jpg?20|}} ** NO SHOWS** - **the charge corresponds to the running cost of the system** |
===== Cell Sorters ===== | ===== Cell Sorters ===== | ||
- Users must [[imm-flowcytometry@medicina.ulisboa.pt|contact us]] to get access to the cell sorters booking. | - Users must [[imm-flowcytometry@medicina.ulisboa.pt|contact us]] to get access to the cell sorters booking. | ||
- | - The cell sorters **can be booked up to 6 weeks in advance** between 10am and 10pm | + | - The cell sorters **can be booked up to 6 weeks in advance** between 10am and 6pm |
- | - Users must **include 15 min in their booking time for purity checks and cleaning** between sorts | + | - Users must **include time in their booking for new settings, purity checks and cleaning** (minimum 30 min) |
- | - Users must mention in the booking comments which nozzle they will be using (70 μm, 85 μm or 100 μm) | + | - Users must mention in the booking comments which nozzle (70 μm, 85 μm or 100 μm) and collection tubes (1.5 mL tubes, 5 mL FACStubes or 15 mL Falcon tubes) they will be using |
- Users must not book on behalf of others | - Users must not book on behalf of others | ||
- Users can book unlimited slots but **can only cancel 7 days in advance** | - Users can book unlimited slots but **can only cancel 7 days in advance** | ||
- | |||
- | {{warning.jpg?20|}} Users will be **billed according to the actual starting time or booked starting time (whichever occurs first) and the actual ending time or booked ending time (whichever occurs last)** | ||
{{warning.jpg?20|}} ** Cancellations** (within 6 days in advance) must be requested by [[imm-flowcytometry@medicina.ulisboa.pt|email]] \\ | {{warning.jpg?20|}} ** Cancellations** (within 6 days in advance) must be requested by [[imm-flowcytometry@medicina.ulisboa.pt|email]] \\ | ||
- | **The penalty charge is 2x the cost/hour of booked time** \\ | + | **The penalty charge corresponds to the running cost of the system if the session is not booked by another user** |
- | (penalty charges will not be applied to clinical samples or mouse samples requiring "same-day" genotyping, but booked time will be charged) | + | \\ |
- | + | {{warning.jpg?20|}} ** NO SHOWS** (1 day before or on the same day) - **the charge corresponds to the running cost of the system** | |
- | {{warning.jpg?20|}} ** NO SHOWS** (1 day before or on the same day) - **the charge is 4x the cost/hour** | + | |
==== Sample Preparation ==== | ==== Sample Preparation ==== | ||
- | Remember to bring: | + | Please check **[[prepare_samples|How to prepare samples for Flow Cytometry]]**. |
- | * correct controls | + | |
- | * filtered cells | + | |
- | * collection tubes | + | |
- | * media for dilution and collection | + | |
===== Abuse Report ===== | ===== Abuse Report ===== |