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usage_policy [2017/01/18 16:18] 10.97.105.213 created |
usage_policy [2017/05/25 17:37] flowcytometry |
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- Users must get trained on a cell analyzer before using it. Use the booking system or [[imm-flowcytometry@medicina.ulisboa.pt|email us]] to request training. | - Users must get trained on a cell analyzer before using it. Use the booking system or [[imm-flowcytometry@medicina.ulisboa.pt|email us]] to request training. | ||
- After training, users must [[imm-flowcytometry@medicina.ulisboa.pt|email us]] their full name, Lab and phone extension to get room access permission | - After training, users must [[imm-flowcytometry@medicina.ulisboa.pt|email us]] their full name, Lab and phone extension to get room access permission | ||
- | - The cell analyzers **can be booked up to 2 months in advance** | + | - The cell analyzers **can be booked up to 6 weeks in advance** |
- | - Users must have a valid reservation in an imaging system before using it. | + | - Users must **include 15 min in their booking time for cleaning** |
+ | - Users must have a valid reservation in a system before using it. | ||
- Users must use their own booked time and not book or use a resource with other username. | - Users must use their own booked time and not book or use a resource with other username. | ||
- Users can book unlimited slots but **can only cancel 3h before the slot starting time** | - Users can book unlimited slots but **can only cancel 3h before the slot starting time** | ||
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- The last booked user of the day is responsible for switching the system off | - The last booked user of the day is responsible for switching the system off | ||
- Users must always export their data, delete it from the database and write down the data transfer in the logsheet | - Users must always export their data, delete it from the database and write down the data transfer in the logsheet | ||
- | - Users must **always clean the machines**, empty the waste reservoir and refill the sheath tank with PBS1X+Azide. | + | - Users must **always clean the machines** following the cleaning protocol, **check if the waste reservoir is full** (empty it if above the limit line) and **check the sheath tank** (refill with PBS1X+Azide if empty). |
+ | |||
+ | {{warning.jpg?20|}} Users will be **billed according to the actual starting time or booked starting time (whichever occurs first) and the actual ending time or booked ending time (whichever occurs last)** | ||
{{warning.jpg?20|}} ** Last Minute Cancellations** (within 3h before the starting booked time) must be requested by [[imm-flowcytometry@medicina.ulisboa.pt|email]] \\ | {{warning.jpg?20|}} ** Last Minute Cancellations** (within 3h before the starting booked time) must be requested by [[imm-flowcytometry@medicina.ulisboa.pt|email]] \\ | ||
- | **The penalty charge is 2x the cost/hour of booked time** \\ | + | **The penalty charge is 2x the cost/hour of booked time if the session is not booked by another user** \\ |
(penalty charges will not be applied to clinical samples or mouse samples requiring "same-day" genotyping, but booked time will be charged) | (penalty charges will not be applied to clinical samples or mouse samples requiring "same-day" genotyping, but booked time will be charged) | ||
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===== Cell Sorters ===== | ===== Cell Sorters ===== | ||
- Users must [[imm-flowcytometry@medicina.ulisboa.pt|contact us]] to get access to the cell sorters booking. | - Users must [[imm-flowcytometry@medicina.ulisboa.pt|contact us]] to get access to the cell sorters booking. | ||
- | - The cell sorters **can be booked up to 2 months in advance** between 10am and 10pm | + | - The cell sorters **can be booked up to 6 weeks in advance** between 10am and 10pm |
- | - Users must include 15 min in their booking time for purity checks and cleaning between sorts | + | - Users must **include 15 min in their booking time for purity checks and cleaning** between sorts |
- Users must mention in the booking comments which nozzle they will be using (70 μm, 85 μm or 100 μm) | - Users must mention in the booking comments which nozzle they will be using (70 μm, 85 μm or 100 μm) | ||
- Users must not book on behalf of others | - Users must not book on behalf of others | ||
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{{warning.jpg?20|}} ** Cancellations** (within 6 days in advance) must be requested by [[imm-flowcytometry@medicina.ulisboa.pt|email]] \\ | {{warning.jpg?20|}} ** Cancellations** (within 6 days in advance) must be requested by [[imm-flowcytometry@medicina.ulisboa.pt|email]] \\ | ||
- | **The penalty charge is 2x the cost/hour of booked time** \\ | + | **The penalty charge is 2x the cost/hour of booked time if the session is not booked by another user** \\ |
(penalty charges will not be applied to clinical samples or mouse samples requiring "same-day" genotyping, but booked time will be charged) | (penalty charges will not be applied to clinical samples or mouse samples requiring "same-day" genotyping, but booked time will be charged) | ||
{{warning.jpg?20|}} ** NO SHOWS** (1 day before or on the same day) - **the charge is 4x the cost/hour** | {{warning.jpg?20|}} ** NO SHOWS** (1 day before or on the same day) - **the charge is 4x the cost/hour** | ||
+ | |||
+ | ==== Sample Preparation ==== | ||
+ | |||
+ | Remember to bring: | ||
+ | * correct controls | ||
+ | * filtered cells | ||
+ | * collection tubes | ||
+ | * media for dilution and collection | ||
===== Abuse Report ===== | ===== Abuse Report ===== |