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resources [2020/04/06 12:26] flowcytometry |
resources [2020/04/23 17:15] flowcytometry |
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<html><img src="/facility/flowcytometry/lib/exe/fetch.php?media=resources.png"><BR><BR></html> | <html><img src="/facility/flowcytometry/lib/exe/fetch.php?media=resources.png"><BR><BR></html> | ||
- | The UCF at iMM is equipped with the following resources: 2 High Speed Cell Sorters, 1 Imaging Flow Cytometer and 4, 10 and 16 colour Cell Analyzers. | + | The UCF at iMM is equipped with several instruments. |
- | <br> | + | The choice of the proper equipment should depend on: |
- | You should choose your cytometer depending not only on the **aims/applications of your project**, but also on the **combination of fluorophores and antibody conjugates** in your samples, and on the availability of the equipment. | + | * the **aims/applications of your project**; |
+ | * the **combination of fluorochromes** in your samples; | ||
+ | * the **optical configuration** required to detect your fluorochrome panel; | ||
+ | * the **availability of the equipment**. | ||
+ | |||
+ | The following table summarizes the excitation optics of all our flow cytometers. To find out more about each instrument's optical configuration, see [[filters|the fluorochromes compatible with the cytometers]] and the individual descriptions bellow. | ||
+ | |||
+ | {{ exc_lasers_imm.jpg?700 }} | ||
{{maps-icon.png?25|}} [[map|Where are the flow cytometers?]] | {{maps-icon.png?25|}} [[map|Where are the flow cytometers?]] | ||
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==== Cell Sorters ==== | ==== Cell Sorters ==== | ||
- | * [[bd_facsaria_iii|BD FACSAria III]] -> <html><a href="https://imm.medicina.ulisboa.pt/intranet/booking/8"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=date.png"> Booking</a></html> | + | * [[bd_facsaria_iii|{{::aria_iii.png?150}}]] [[bd_facsaria_iii|BD FACSAria III]] -> <html><a href="https://imm.medicina.ulisboa.pt/intranet/booking/8"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=date.png"> Booking</a></html> |
- | * [[bd_facsaria_iiu|BD FACSAria IIu]] -> <html><a href="https://imm.medicina.ulisboa.pt/intranet/booking/44"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=date.png"> Booking</a></html> | + | * [[bd_facsaria_iiu|{{::aria_iiu.png?150|}}]] [[bd_facsaria_iiu|BD FACSAria IIu]] -> <html><a href="https://imm.medicina.ulisboa.pt/intranet/booking/44"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=date.png"> Booking</a></html> |
==== Cell Analyzers ==== | ==== Cell Analyzers ==== | ||
- | * [[bd_lsr_fortessa_1|BD LSRFortessa 1]] -> <html><a href="https://imm.medicina.ulisboa.pt/intranet/booking/72"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=date.png"> Booking</a></html> | + | * [[bd_lsr_fortessa_1|{{::fortessa_1.png?120|}}]] [[bd_lsr_fortessa_1|BD LSRFortessa 1]] -> <html><a href="https://imm.medicina.ulisboa.pt/intranet/booking/72"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=date.png"> Booking</a></html> |
- | * [[bd_lsr_fortessa_2|BD LSRFortessa 2]] -> <html><a href="https://imm.medicina.ulisboa.pt/intranet/booking/73"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=date.png"> Booking</a></html> | + | * [[bd_lsr_fortessa_2|{{::fortessa_2.png?120|}}]] [[bd_lsr_fortessa_2|BD LSRFortessa 2]] -> <html><a href="https://imm.medicina.ulisboa.pt/intranet/booking/73"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=date.png"> Booking</a></html> |
- | * [[bd_lsr_fortessa_x20|BD LSRFortessa X-20]] -> <html><a href="https://imm.medicina.ulisboa.pt/intranet/booking/144"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=date.png"> Booking</a></html> | + | * [[bd_lsr_fortessa_x20|{{::fortessa_x20.png?120|}}]] [[bd_lsr_fortessa_x20|BD LSRFortessa X-20]] -> <html><a href="https://imm.medicina.ulisboa.pt/intranet/booking/144"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=date.png"> Booking</a></html> |
- | * [[bd_accuri_c6|BD Accuri C6]] -> <html><a href="https://imm.medicina.ulisboa.pt/intranet/booking/121"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=date.png"> Booking</a></html> | + | * [[bd_accuri_c6|{{::accuri.png?100|}}]] [[bd_accuri_c6|BD Accuri C6]] -> <html><a href="https://imm.medicina.ulisboa.pt/intranet/booking/121"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=date.png"> Booking</a></html> |
==== Imaging Flow Cytometer ==== | ==== Imaging Flow Cytometer ==== | ||
- | * [[ImageStreamX|Amnis ImageStreamX]] -> <html><a href="https://imm.medicina.ulisboa.pt/intranet/booking/145"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=date.png"> Booking</a></html> | + | * [[ImageStreamX|{{::imagestream.png?120|}}]] [[ImageStreamX|Amnis ImageStreamX]] -> <html><a href="https://imm.medicina.ulisboa.pt/intranet/booking/145"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=date.png"> Booking</a></html> |
==== Workstations ==== | ==== Workstations ==== | ||
- | * [[macflow|MacFlow]] - Analysis workstation -> <html><a href="https://imm.medicina.ulisboa.pt/intranet/booking/7"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=date.png"> Booking</a></html> | + | * [[macflow|{{::macflow.png?100|}}]] [[macflow|MacFlow]] - Analysis workstation -> <html><a href="https://imm.medicina.ulisboa.pt/intranet/booking/7"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=date.png"> Booking</a></html> |
==== No longer available... ==== | ==== No longer available... ==== |