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resources [2017/02/10 00:58] flowcytometry |
resources [2020/04/06 12:25] flowcytometry |
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- | <html><img src="/facility/flowcytometry/lib/exe/fetch.php?media=flow_resources.jpg"><BR><BR></html> | + | <html><img src="/facility/flowcytometry/lib/exe/fetch.php?media=resources.png"><BR><BR></html> |
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+ | The UCF at iMM is equipped with the following resources: 2 High Speed Cell Sorters, 1 Imaging Flow Cytometer and 4, 10 and 16 colour Cell Analyzers. | ||
+ | The main major applications within the Flow Cytometry are multicolour phenotyping (we can measure up to 16 colours on an individual cell), DNA analysis and cell cycle kinetics, cell death and apoptosis. | ||
+ | You should choose your cytometer depending not only on the **aims/applications of your project**, but also on the **combination of fluorophores and antibody conjugates** in your samples, and on the availability of the equipment. | ||
{{maps-icon.png?25|}} [[map|Where are the flow cytometers?]] | {{maps-icon.png?25|}} [[map|Where are the flow cytometers?]] | ||
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+ | {{list-icon.png?23|}} [[quality|How are the flow cytometers?]] (Quality Control) | ||
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+ | {{:info.gif?25|}} [[filters|Which fluorochromes are compatible with the cytometers?]] | ||
==== Cell Sorters ==== | ==== Cell Sorters ==== | ||
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* [[bd_lsr_fortessa_x20|BD LSRFortessa X-20]] -> <html><a href="https://imm.medicina.ulisboa.pt/intranet/booking/144"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=date.png"> Booking</a></html> | * [[bd_lsr_fortessa_x20|BD LSRFortessa X-20]] -> <html><a href="https://imm.medicina.ulisboa.pt/intranet/booking/144"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=date.png"> Booking</a></html> | ||
* [[bd_accuri_c6|BD Accuri C6]] -> <html><a href="https://imm.medicina.ulisboa.pt/intranet/booking/121"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=date.png"> Booking</a></html> | * [[bd_accuri_c6|BD Accuri C6]] -> <html><a href="https://imm.medicina.ulisboa.pt/intranet/booking/121"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=date.png"> Booking</a></html> | ||
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+ | ==== Imaging Flow Cytometer ==== | ||
+ | * [[ImageStreamX|Amnis ImageStreamX]] -> <html><a href="https://imm.medicina.ulisboa.pt/intranet/booking/145"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=date.png"> Booking</a></html> | ||
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==== Workstations ==== | ==== Workstations ==== | ||
- | * [[mac_1|Mac 1]] - Mac workstation 1 -> <html><a href="https://imm.medicina.ulisboa.pt/intranet/booking/6"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=date.png"> Booking</a></html> | + | * [[macflow|MacFlow]] - Analysis workstation -> <html><a href="https://imm.medicina.ulisboa.pt/intranet/booking/7"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=date.png"> Booking</a></html> |
- | * [[mac_2|Mac 2]] - Mac workstation 2 -> <html><a href="https://imm.medicina.ulisboa.pt/intranet/booking/7"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=date.png"> Booking</a></html> | + | |
+ | ==== No longer available... ==== | ||
+ | * [[mac_1|Mac 1]] -> retired | ||
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[[start|Back to the Flow Cytometry Wiki]] | [[start|Back to the Flow Cytometry Wiki]] |