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--- resources [2017/01/09 18:40]
10.105.85.57 created
+++ resources [2021/11/15 12:10]
flowcytometry [Cell Sorters]
@@ Line 1: / Line 1: @@
-<html><img src="/facility/flowcytometry/lib/exe/fetch.php?media=flow_resources.jpg"><BR><BR></html>
+<html><img src="/facility/flowcytometry/lib/exe/fetch.php?media=resources.png"><BR><BR></html>
+The UCF at iMM is equipped with several instruments.
+The choice of the proper equipment should depend on:
+  * the **aims/applications of your project**;
+  * the **combination of fluorochromes** in your samples;
+  * the **optical configuration** required to detect your fluorochrome panel;
+  * the **availability of the equipment**.
+The following table summarizes the excitation optics of all our flow cytometers. To find out more about each instrument's optical configuration, see [[filters|the fluorochromes compatible with the cytometers]] and the individual descriptions bellow.
+{{ exc_lasers_imm.jpg?700 }}
 {{maps-icon.png?25|}} [[map|Where are the flow cytometers?]]
+{{list-icon.png?23|}} [[quality|How are the flow cytometers?]] (Quality Control)
+{{:info.gif?25|}} [[filters|Which fluorochromes are compatible with the cytometers?]]
 ==== Cell Sorters ====
-  * [[bd_facsaria_iii|BD FACSAria III]] (housed in a Baker BioProtect-IV bio-safety cabinet) -> <html><a href="https://imm.medicina.ulisboa.pt/intranet/booking/8"><img src="/facility/bioimaging/lib/exe/fetch.php?media=date.png"> Booking</a></html>
-  * [[bd_facsaria_iiu|BD FACSAria IIu]] -> <html><a href="https://imm.medicina.ulisboa.pt/intranet/booking/44"><img src="/facility/bioimaging/lib/exe/fetch.php?media=date.png"> Booking</a></html>
-==== Analyzers ====
+  * [[bd_facsaria_fusion|{{::bd_facs_aria_fusion_wiki.png?150}}]] [[bd_facsaria_fusion|BD FACSAria Fusion]] -> <html><a href="https://my.agendoscience.com/calendar/?id=MTYyY2FsZW5kYXJz="><img src="/facility/flowcytometry/lib/exe/fetch.php?media=date.png"> Booking</a></html>
-  * [[bd_lsr_fortessa_1|BD LSRFortessa 1]] -> <html><a href="https://imm.medicina.ulisboa.pt/intranet/booking/72"><img src="/facility/bioimaging/lib/exe/fetch.php?media=date.png"> Booking</a></html>
+  * [[bd_facsaria_iii|{{::aria_iii.png?150}}]] [[bd_facsaria_iii|BD FACSAria III]] -> <html><a href="https://my.agendo.science/calendar/?id=NDVjYWxlbmRhcnM="><img src="/facility/flowcytometry/lib/exe/fetch.php?media=date.png"> Booking</a></html>
-  * [[bd_lsr_fortessa_2|BD LSRFortessa 2]] -> <html><a href="https://imm.medicina.ulisboa.pt/intranet/booking/73"><img src="/facility/bioimaging/lib/exe/fetch.php?media=date.png"> Booking</a></html>
-  * [[bd_accuri_c6|BD Accuri C6]] -> <html><a href="https://imm.medicina.ulisboa.pt/intranet/booking/121"><img src="/facility/bioimaging/lib/exe/fetch.php?media=date.png"> Booking</a></html>
+==== Cell Analyzers ====
+  * [[bd_lsr_fortessa_1|{{::fortessa_1.png?120|}}]] [[bd_lsr_fortessa_1|BD LSRFortessa 1]] -> <html><a href="https://my.agendo.science/calendar/?id=NDBjYWxlbmRhcnM="><img src="/facility/flowcytometry/lib/exe/fetch.php?media=date.png"> Booking</a></html>
+  * [[bd_lsr_fortessa_2|{{::fortessa_2.png?120|}}]] [[bd_lsr_fortessa_2|BD LSRFortessa 2]] -> <html><a href="https://my.agendo.science/calendar/?id=NDFjYWxlbmRhcnM="><img src="/facility/flowcytometry/lib/exe/fetch.php?media=date.png"> Booking</a></html>
+  * [[bd_lsr_fortessa_x20|{{::fortessa_x20.png?120|}}]] [[bd_lsr_fortessa_x20|BD LSRFortessa X-20]] -> <html><a href="https://my.agendo.science/calendar/?id=NDJjYWxlbmRhcnM="><img src="/facility/flowcytometry/lib/exe/fetch.php?media=date.png"> Booking</a></html>
+  * [[bd_accuri_c6|{{::accuri.png?120|}}]] [[bd_accuri_c6|BD Accuri C6 Plus]] -> <html><a href="https://my.agendo.science/calendar/?id=NDNjYWxlbmRhcnM="><img src="/facility/flowcytometry/lib/exe/fetch.php?media=date.png"> Booking</a></html>
+==== Imaging Flow Cytometer ====
+  * [[ImageStreamX|{{::imagestream.png?120|}}]] [[ImageStreamX|Amnis ImageStreamX]] -> <html><a href="https://my.agendo.science/calendar/?id=MTBjYWxlbmRhcnM="><img src="/facility/flowcytometry/lib/exe/fetch.php?media=date.png"> Booking</a></html>
 ==== Workstations ====
-  * [[mac_1|Mac 1]] - Mac workstation 1 -> <html><a href="https://imm.medicina.ulisboa.pt/intranet/booking/6"><img src="/facility/bioimaging/lib/exe/fetch.php?media=date.png"> Booking</a></html>
+{{::macflow.png?100|}}
-  * [[mac_2|Mac 2]] - Mac workstation 2 -> <html><a href="https://imm.medicina.ulisboa.pt/intranet/booking/7"><img src="/facility/bioimaging/lib/exe/fetch.php?media=date.png"> Booking</a></html>
+  * **MacFlow 1** - With FlowJo Software -> <html><a href="https://my.agendo.science/calendar/?id=NDZjYWxlbmRhcnM="><img src="/facility/flowcytometry/lib/exe/fetch.php?media=date.png"> Booking</a></html>
+  * **MacFlow 2 (new)** - With FlowJo Software -> <html><a href="https://my.agendo.science/calendar/?id=NDZjYWxlbmRhcnM="><img src="/facility/flowcytometry/lib/exe/fetch.php?media=date.png"> Booking</a></html>
+{{:pc_workstation.jpg?100|}}
+  * **PC** - With FlowJo, FACSDiva and ModFitLT Software -> <html><a href="https://my.agendo.science/calendar/?id=MTUxY2FsZW5kYXJz"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=date.png"> Booking</a></html>
+==== No longer available ====
+  * [[bd_facsaria_iiu|{{::aria_iiu.png?150|}}]] [[bd_facsaria_iiu|BD FACSAria IIu]] -> <html><a href="https://my.agendo.science/calendar/?id=NDRjYWxlbmRhcnM="><img src="/facility/flowcytometry/lib/exe/fetch.php?media=date.png"> Booking</a></html>
 ----
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