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imagestreamx [2020/06/01 20:53] flowcytometry |
imagestreamx [2023/03/31 12:49] flowcytometry [Additional information] |
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- | <img src="/facility/flowcytometry/lib/exe/fetch.php?media=imagestreamx_name.jpg"> | + | <img src="/facility/flowcytometry/lib/exe/fetch.php?media=amnis_nome.png"> |
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<td style="border:0px solid white;"> <p style="line-height:1.8"> | <td style="border:0px solid white;"> <p style="line-height:1.8"> | ||
<b>Location</b>: <a href="/facility/flowcytometry/lib/exe/fetch.php?media=flow_cytometry_map.jpg">Room P2-A-49</a> (<img src="/facility/flowcytometry/lib/exe/fetch.php?media=telephone_icon.gif" width=15> 47224) <br> | <b>Location</b>: <a href="/facility/flowcytometry/lib/exe/fetch.php?media=flow_cytometry_map.jpg">Room P2-A-49</a> (<img src="/facility/flowcytometry/lib/exe/fetch.php?media=telephone_icon.gif" width=15> 47224) <br> | ||
- | <b>Manufacturer</b>: <a href="http://www.merckmillipore.com/PT/en" target="_blank">Merck</a><br> | + | <b>Manufacturer</b>: <a href="https://www.luminexcorp.com/">Luminex</a><br> |
- | <b>Model</b>: <a href="http://www.merckmillipore.com/PT/en/life-science-research/cell-analysis-flow-cytometry/amnis-imaging-flow-cytometers/imagestreamx-Mark-ii-imaging-flow-cytometer/VaSb.qB.QokAAAFLzRop.zHe,nav" target="_blank">Amnis ImageStreamX Mark II</a><br> | + | <b>Model</b>: <a href="https://www.luminexcorp.com/imagestreamx-mk-ii/#overview" target="_blank">Amnis ImageStreamX Mark II</a><br> |
<b>Nickname</b>: "ImageStream"<br> | <b>Nickname</b>: "ImageStream"<br> | ||
<b>Software</b>: INSPIRE 200.1.620.0 / IDEAS 6.2.64.0<br> | <b>Software</b>: INSPIRE 200.1.620.0 / IDEAS 6.2.64.0<br> | ||
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* **[[prepare_samples|How to prepare samples for Flow Cytometry]]** | * **[[prepare_samples|How to prepare samples for Flow Cytometry]]** | ||
- | * {{::imagestreamx_sample_preparation_guide.pdf| Sample Preparation Guide}}. **Note**: Samples are run using 1.5 ml microfuge tubes (Sarstedt ref. 72706) {{::microfuge_tube.jpg?60|}} | + | * [[https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.ucf.026_-_amnis_imagextreamx_operation.pdf| Amnis ImageStreamX Operation]] |
+ | * {{::imagestreamx_sample_preparation_guide.pdf| Sample Preparation Guide}}. **Note**: Samples are run using 1.5 ml microfuge tubes (Sarstedt ref. 72706) | ||
* {{::imagestreamx_brochure.pdf| ImageStreamX Brochure}} | * {{::imagestreamx_brochure.pdf| ImageStreamX Brochure}} | ||
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Please check the {{ ::sop.ucf.004_-_ucf_imm_user_guidelines.pdf |User Guidelines}} for detailed information. | Please check the {{ ::sop.ucf.004_-_ucf_imm_user_guidelines.pdf |User Guidelines}} for detailed information. | ||
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- | ===== Cleaning Protocol ===== | ||
- | |||
- | **Are you done?** Just click on ''Shutdown'' and select ''Shudown after sterilize''. The ImageStreamX will automatically turn off all illumination sources and rinse | ||
- | the entire fluidic system with water, sterilizer, cleanser, de-bubbler, and then | ||
- | water again. The sterilizer is held in the system for ten minutes to ensure decontamination. | ||
- | |||
- | You can walk away from the system. It takes about 45 minutes of unattended operation to complete. | ||
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[[resources|Back to the Resources page]] | [[resources|Back to the Resources page]] |