This shows you the differences between two versions of the page.
Both sides previous revision Previous revision Next revision | Previous revision Next revision Both sides next revision | ||
imagestreamx [2018/06/08 18:12] flowcytometry [Additional information] |
imagestreamx [2020/06/01 20:53] flowcytometry |
||
---|---|---|---|
Line 4: | Line 4: | ||
<tr style="border:0px solid white;"> | <tr style="border:0px solid white;"> | ||
<td style="border:0px solid white;"> | <td style="border:0px solid white;"> | ||
- | <a href="/facility/flowcytometry/lib/exe/fetch.php?media=imagestreamx_wiki.jpg"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=imagestreamx_wiki.jpg" width=300></a> | + | <a href="/facility/flowcytometry/lib/exe/fetch.php?media=imagestreamx_wiki.png"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=imagestreamx_wiki.png" width=300></a> |
<td style="border:0px solid white;"> <p style="line-height:1.8"> | <td style="border:0px solid white;"> <p style="line-height:1.8"> | ||
<b>Location</b>: <a href="/facility/flowcytometry/lib/exe/fetch.php?media=flow_cytometry_map.jpg">Room P2-A-49</a> (<img src="/facility/flowcytometry/lib/exe/fetch.php?media=telephone_icon.gif" width=15> 47224) <br> | <b>Location</b>: <a href="/facility/flowcytometry/lib/exe/fetch.php?media=flow_cytometry_map.jpg">Room P2-A-49</a> (<img src="/facility/flowcytometry/lib/exe/fetch.php?media=telephone_icon.gif" width=15> 47224) <br> | ||
Line 17: | Line 17: | ||
  <br> |   <br> | ||
  <br> |   <br> | ||
- | → <a href="https://imm.medicina.ulisboa.pt/intranet/booking/145"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=date.png"> Amnis ImageStreamX Booking</a> | + | → <a href="https://my.agendo.science/calendar/?id=MTBjYWxlbmRhcnM="><img src="/facility/flowcytometry/lib/exe/fetch.php?media=date.png"> Amnis ImageStreamX Booking</a> |
<br> | <br> | ||
→ <a href="/facility/flowcytometry/doku.php?id=amnis_imagestreamx_usage"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=chart_line.png"> Amnis ImageStreamX Usage Statistics</a> | → <a href="/facility/flowcytometry/doku.php?id=amnis_imagestreamx_usage"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=chart_line.png"> Amnis ImageStreamX Usage Statistics</a> | ||
Line 40: | Line 40: | ||
{{::imagestreamx_fluorochrome_chart.png?700|}} | {{::imagestreamx_fluorochrome_chart.png?700|}} | ||
+ | |||
+ | ===== Objectives Information ===== | ||
+ | |||
+ | ^ Magnification ^ 20x ^ 40x ^ 60x ^ | ||
+ | ^ Numerical Aperture | 0.50 | 0.75 | 0.90 | | ||
+ | ^ Field of View (μm) | 120 | 60 | 40 | | ||
+ | ^ Pixel Size (μm) | 1.0 | 0.5 | 0.33 | | ||
+ | ^ Depth of Field (μm) | 8.0 | 4.0 | 2.5 | | ||
+ | |||
+ | ===== Image Acquisition Performance ===== | ||
+ | |||
+ | {{::high_speed_imaging.png?600|}} | ||
===== Additional information ===== | ===== Additional information ===== | ||
Line 52: | Line 64: | ||
Please check the {{ ::sop.ucf.004_-_ucf_imm_user_guidelines.pdf |User Guidelines}} for detailed information. | Please check the {{ ::sop.ucf.004_-_ucf_imm_user_guidelines.pdf |User Guidelines}} for detailed information. | ||
+ | |||
+ | ===== Cleaning Protocol ===== | ||
+ | |||
+ | **Are you done?** Just click on ''Shutdown'' and select ''Shudown after sterilize''. The ImageStreamX will automatically turn off all illumination sources and rinse | ||
+ | the entire fluidic system with water, sterilizer, cleanser, de-bubbler, and then | ||
+ | water again. The sterilizer is held in the system for ten minutes to ensure decontamination. | ||
+ | |||
+ | You can walk away from the system. It takes about 45 minutes of unattended operation to complete. | ||
---- | ---- | ||
[[resources|Back to the Resources page]] | [[resources|Back to the Resources page]] |