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imagestreamx [2018/06/08 13:51] flowcytometry |
imagestreamx [2018/06/08 18:36] flowcytometry [Cleaning Protocol] |
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* **Multimag**: 20x, 40x and 60x magnification | * **Multimag**: 20x, 40x and 60x magnification | ||
* **Extended depth of field**: projects all structures within a cell into a single plane of focus | * **Extended depth of field**: projects all structures within a cell into a single plane of focus | ||
+ | |||
+ | {{ ::img-12channels.jpg |}} | ||
===== Configuration ===== | ===== Configuration ===== | ||
+ | {{::imagestreamx_fluorochrome_chart.png?700|}} | ||
+ | ===== Objectives Information ===== | ||
+ | |||
+ | ^ Magnification ^ 20x ^ 40x ^ 60x ^ | ||
+ | ^ Numerical Aperture | 0.50 | 0.75 | 0.90 | | ||
+ | ^ Field of View (μm) | 120 | 60 | 40 | | ||
+ | ^ Pixel Size (μm) | 1.0 | 0.5 | 0.33 | | ||
+ | ^ Depth of Field (μm) | 8.0 | 4.0 | 2.5 | | ||
+ | |||
+ | ===== Image Acquisition Performance ===== | ||
+ | |||
+ | {{::high_speed_imaging.png?700|}} | ||
+ | |||
+ | ===== Additional information ===== | ||
+ | |||
+ | * **[[prepare_samples|How to prepare samples for Flow Cytometry]]** | ||
+ | * {{::imagestreamx_sample_preparation_guide.pdf| Sample Preparation Guide}}. **Note**: Samples are run using 1.5 ml microfuge tubes (Sarstedt ref. 72706) {{::microfuge_tube.jpg?60|}} | ||
+ | * {{::imagestreamx_brochure.pdf| ImageStreamX Brochure}} | ||
===== Booking Rules ===== | ===== Booking Rules ===== | ||
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Please check the {{ ::sop.ucf.004_-_ucf_imm_user_guidelines.pdf |User Guidelines}} for detailed information. | Please check the {{ ::sop.ucf.004_-_ucf_imm_user_guidelines.pdf |User Guidelines}} for detailed information. | ||
+ | |||
+ | ===== Cleaning Protocol ===== | ||
+ | |||
+ | **Are you done?** Just click on ''Shutdown''. The ImageStreamX will automatically turn off all illumination sources and rinse | ||
+ | the entire fluidic system with water, sterilizer, cleanser, de-bubbler, and then | ||
+ | water again. The sterilizer is held in the system for ten minutes to ensure decontamination. | ||
+ | |||
+ | You can walk away from the system. It takes about 45 minutes of unattended operation to complete. | ||
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[[resources|Back to the Resources page]] | [[resources|Back to the Resources page]] |