bd_facsaria_iii
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bd_facsaria_iii [2021/11/15 18:28] flowcytometry |
bd_facsaria_iii [2021/11/15 18:30] flowcytometry |
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| Sorting can be performed into two or four tubes with speeds up to 25,000 events/second. The system sorts by incorporating cells from the sample tube into a stream of sterile PBS. The stream is interrogated by the lasers at the flow cell and the system electronics keeps track of each cell as they pass through the laser and determines specific cells that meet the sort criteria. A transducer vibrates the stream and induces droplet formation, with cells in the stream being incorporated into the droplets. If a cell meets the sorting criteria and is in the last drop before the break off, the instrument will charge that drop. The charged droplet is then deflected into the proper collection tube by the charge plates. Different cell types can be sorted with the use of 70 μm, 85 μm and 100 μm nozzles. | Sorting can be performed into two or four tubes with speeds up to 25,000 events/second. The system sorts by incorporating cells from the sample tube into a stream of sterile PBS. The stream is interrogated by the lasers at the flow cell and the system electronics keeps track of each cell as they pass through the laser and determines specific cells that meet the sort criteria. A transducer vibrates the stream and induces droplet formation, with cells in the stream being incorporated into the droplets. If a cell meets the sorting criteria and is in the last drop before the break off, the instrument will charge that drop. The charged droplet is then deflected into the proper collection tube by the charge plates. Different cell types can be sorted with the use of 70 μm, 85 μm and 100 μm nozzles. | ||
| - | If you need to sort potentially hazardous samples (e.g human samples and other BSL-2 level samples) you need to sort in tha BD FACSAria Fusion, equiped with a | + | **If you need to sort potentially hazardous samples (e.g human samples and other BSL-2 level samples) you need to sort in the BD FACSAria Fusion, equiped with a Class II Type A2 biosafety cabinet to reduce the risk of operator exposure.** |
| * Three lasers with blue (488 nm), yellow-green (561 nm) and red (633 nm) | * Three lasers with blue (488 nm), yellow-green (561 nm) and red (633 nm) | ||
bd_facsaria_iii.txt · Last modified: 2024/06/10 23:03 by flowcytometry
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