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bd_accuri_c6 [2017/01/18 13:50] 10.97.105.213 |
bd_accuri_c6 [2020/01/06 15:43] flowcytometry [Additional Information] |
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- | <a href="/facility/flowcytometry/lib/exe/fetch.php?media=accuri_c6_wiki.jpg"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=accuri_c6_wiki.jpg" width=300></a> | + | <a href="/facility/flowcytometry/lib/exe/fetch.php?media=accuri_c6_wiki.png"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=accuri_c6_wiki.png" width=300></a> |
<td style="border:0px solid white;"> <p style="line-height:1.8"> | <td style="border:0px solid white;"> <p style="line-height:1.8"> | ||
- | <b>Location</b>: <a href="/facility/flowcytometry/lib/exe/fetch.php?media=flow_cytometry_map.jpg">Room P2-A-49</a> (<img src="/facility/flowcytometry/lib/exe/fetch.php?media=telephone_icon.gif" width=15> 47222) <br> | + | <b>Location</b>: <a href="/facility/flowcytometry/lib/exe/fetch.php?media=flow_cytometry_map.jpg">Room P2-A-49</a> (<img src="/facility/flowcytometry/lib/exe/fetch.php?media=telephone_icon.gif" width=15> 47224) <br> |
<b>Manufacturer</b>: <a href="http://www.bdbiosciences.com/eu/home" target="_blank">BD Biosciences</a><br> | <b>Manufacturer</b>: <a href="http://www.bdbiosciences.com/eu/home" target="_blank">BD Biosciences</a><br> | ||
<b>Model</b>: <a href="http://www.bdbiosciences.com/us/instruments/s/accuric6" target="_blank">Accuri C6</a><br> | <b>Model</b>: <a href="http://www.bdbiosciences.com/us/instruments/s/accuric6" target="_blank">Accuri C6</a><br> | ||
+ | <b>Nickname</b>: "Accuri"<br> | ||
<b>Software</b>: Accuri C6 software 1.0.264.21<br> | <b>Software</b>: Accuri C6 software 1.0.264.21<br> | ||
<b>Year</b>: 2015 <br> | <b>Year</b>: 2015 <br> | ||
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- | → <a href="https://intranet.imm.medicina.ulisboa.pt/c/document_library/get_file?p_l_id=183913&folderId=536842&name=DLFE-22134.pdf"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=info.gif" width=16> BD Accuri C6 User Guidelines</a><br> | ||
→ <a href="https://imm.medicina.ulisboa.pt/intranet/booking/121"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=date.png"> BD Accuri C6 Booking</a> | → <a href="https://imm.medicina.ulisboa.pt/intranet/booking/121"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=date.png"> BD Accuri C6 Booking</a> | ||
<br> | <br> | ||
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===== System overview ===== | ===== System overview ===== | ||
- | The BD Accuri C6 //cell analyzer// is an easy-to-use benchtop flow cytometer that allows for absolute cell counting and has a flexible optical layout. It has two lasers - 488 nm and 633 nm and four fluorescence detectors. The BD Accuri C6 can acquire up to 10,000 events/second, with the data being digitally collected over a 7-decade dynamic range. Its low pressure pumping system allows for acquisition with any type of device (e.g. FACS, Eppendorf or Falcon tubes). Since the sample tube remains open (is not pressurized), the Accuri C6 allows for addition of compounds without interruption in sampling. **The fluidics system derives sample volume and can determine absolute cell counts** or sample concentration per microliter. If you need a cell analyzer with more colors, check the [[bd_lsr_fortessa_1|BD LSRFortessa 1]] instead. | + | The BD Accuri C6 //cell analyzer// is an easy-to-use benchtop flow cytometer that allows for absolute cell counting and has a flexible optical layout. It has two lasers - 488 nm and 640 nm and four fluorescence detectors. The BD Accuri C6 can acquire up to 10,000 events/second, with the data being digitally collected over a 7-decade dynamic range. Its low pressure pumping system allows for acquisition with any type of device (e.g. FACS, Eppendorf or Falcon tubes). Since the sample tube remains open (is not pressurized), the Accuri C6 allows for addition of compounds without interruption in sampling. **The fluidics system derives sample volume and can determine absolute cell counts** or sample concentration per microliter. If you need a cell analyzer with more colors, check the [[bd_lsr_fortessa_1|BD LSRFortessa 1]] instead. |
- | * Two lasers: blue (488 nm) and red (633 nm) | + | * Two lasers: blue (488 nm) and red (640 nm) |
* Multicolor analysis of up to 4 parameters | * Multicolor analysis of up to 4 parameters | ||
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Default configuration: | Default configuration: | ||
- | {{::accuri_default.png|}} | + | {{::accuri_default.png?620|}} |
+ | \\ | ||
+ | \\ | ||
+ | {{::bd_accuri_c6_488.png?700|}} | ||
+ | \\ | ||
+ | {{::bd_accuri_c6_640.png?700|}} | ||
- | Check [[https://imm.medicina.ulisboa.pt/files/6614/6348/1789/BD_Accuri_C6_Configurations.pdf|the list of possible configurations]]. | + | Check [[https://imm.medicina.ulisboa.pt/files/5614/8700/1592/FORM.UCF.048_-_BD_Accuri_C6_Configurations.pdf|the list of possible configurations]]. |
+ | |||
+ | ===== Additional Information ===== | ||
+ | |||
+ | * **[[prepare_samples|How to prepare samples for Flow Cytometry]]** | ||
+ | * [[https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=form.ucf.049_-_bd_accuri_c6_operation.pdf| BD Accuri C6 Operation]] | ||
===== Booking Rules ===== | ===== Booking Rules ===== | ||
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Please check the {{::sop.ucf.004_-_ucf_imm_user_guidelines.pdf |User Guidelines}} for detailed information. | Please check the {{::sop.ucf.004_-_ucf_imm_user_guidelines.pdf |User Guidelines}} for detailed information. | ||
+ | |||
+ | ===== Cleaning Protocol ===== | ||
+ | |||
+ | {{::cleaning_procedure_accuri.png?700|}} | ||
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[[resources|Back to the Resources page]] | [[resources|Back to the Resources page]] |