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about [2018/07/21 01:13]
flowcytometry
about [2020/04/28 12:41]
flowcytometry [About the Flow Cytometry Unit]
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-====== About the Flow Cytometry ​Facility ​======+====== About the Flow Cytometry ​Unit ======
  
-The Flow Cytometry ​Facility ​provides a state of the art flow cytometry service to iMM Lisboa ​researchers as well as external groups from other research institutes. We aim at training researchers in the adequate use of the instruments and also on flow cytometry principles, experiment planning, as well as data analysis and interpretation. The Flow Cytometry Facility also provides a cell sorting service using two High Speed Cell Sorters (BD FACSAria III and BD FACSAria IIu) operated by dedicated staff.+{{::​ucf_2019.jpg?​600 ​ |}} \\ \\ \\ \\ \\ \\ The Flow Cytometry ​Unit provides a state of the art flow cytometry service to iMM researchers as well as external groups from other research institutes. We aim at training researchers in the adequate use of the instruments and also on flow cytometry principles, experiment planning, as well as data analysis and interpretation. The Flow Cytometry Facility also provides a cell sorting service using two High Speed Cell Sorters (BD FACSAria III and BD FACSAria IIu) operated by dedicated staff. 
 +\\ 
 +Photo: [[https://​www.jorgefiganiercastro.com/​|Jorge Figanier Castro]]
  
-===== Team =====+\\
  
-{{::​flow_cytometry_team.jpg?​650|}}+===== Team =====
  
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-<div align="​center"><​b>​Head of Facility</​b></​div>​+<div align="​center"><​b>​Head of Unit</​b></​div>​
 <br> <br>
 <div style="​width:​ 150px; height: 150px; overflow: hidden; position: relative;"><​img src="/​facility/​flowcytometry/​lib/​exe/​fetch.php?​media=jose_rino.png"​ style="​width:​150px;​ height: 150px;"​ /></​div><​br><​br>​ <div style="​width:​ 150px; height: 150px; overflow: hidden; position: relative;"><​img src="/​facility/​flowcytometry/​lib/​exe/​fetch.php?​media=jose_rino.png"​ style="​width:​150px;​ height: 150px;"​ /></​div><​br><​br>​
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-<​b>​José Rino</​b>​ has a <​i>​Licenciatura</​i>​ in Technological Physics Engineering (Instituto Superior Técnico) and a PhD in Biophysics (Faculdade de Ciências). José was appointed Head of the Flow Cytometry ​Facility ​in October 2016. He is also Head of the Bioimaging ​Facility ​at iMM. <a href="​https://​imm.medicina.ulisboa.pt/​facility/​bioimaging/​doku.php?​id=facility_bioimaging_doku.php&​id=rino_cv">​More information</​a>​.<​br>​+<​b>​José Rino</​b>​ has a <​i>​Licenciatura</​i>​ in Technological Physics Engineering (Instituto Superior Técnico) and a PhD in Biophysics (Faculdade de Ciências). José was appointed Head of the Flow Cytometry ​Unit in October 2016. He is also Head of the Bioimaging ​Unit at iMM. <a href="​https://​imm.medicina.ulisboa.pt/​facility/​bioimaging/​doku.php?​id=facility_bioimaging_doku.php&​id=rino_cv">​More information</​a>​.<​br>​
 <img src="​https://​imm.medicina.ulisboa.pt/​facility/​flowcytometry/​lib/​exe/​fetch.php?​media=mail_icon.png"​ width=20> ​ <img src="​https://​imm.medicina.ulisboa.pt/​facility/​flowcytometry/​lib/​exe/​fetch.php?​media=mail_icon.png"​ width=20> ​
  <​i>​joserino@medicina.ulisboa.pt</​i>​  <​i>​joserino@medicina.ulisboa.pt</​i>​
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-<​b>​Andreia Santos</​b>​ has a BSc in Biology and a MSc in Genetic and Molecular Biology from Faculdade de Ciências. Andreia joined the Flow Cytometry ​Facility ​in September 2017 after working at Ana Espada de Sousa’s lab on human T-cell development,​ namely the role of IL-4 in the lineage plasticity of human CD4 single positive thymocytes.<​br>​+<​b>​Andreia Santos</​b>​ has a BSc in Biology and a MSc in Genetic and Molecular Biology from Faculdade de Ciências, Universidade de Lisboa. Andreia joined the Flow Cytometry ​Unit in September 2017 after working at Ana Espada de Sousa’s lab on human T-cell development,​ namely the role of IL-4 in the lineage plasticity of human CD4 single positive thymocytes.<​br>​
 <img src="​https://​imm.medicina.ulisboa.pt/​facility/​flowcytometry/​lib/​exe/​fetch.php?​media=mail_icon.png"​ width=20> ​ <img src="​https://​imm.medicina.ulisboa.pt/​facility/​flowcytometry/​lib/​exe/​fetch.php?​media=mail_icon.png"​ width=20> ​
  <​i>​assantos@medicina.ulisboa.pt</​i>​  <​i>​assantos@medicina.ulisboa.pt</​i>​
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-<div style="​width:​ 150px; height: 150px; overflow: hidden; position: relative;"><​img src="/​facility/​flowcytometry/​lib/​exe/​fetch.php?​media=barbara_oliveira.png" style="​width:​150px;​ height: 150px;"​ /></​div><​br><​br>​+<div style="​width:​ 150px; height: 150px; overflow: hidden; position: relative;"><​img src="/​facility/​flowcytometry/​lib/​exe/​fetch.php?​media=rute_goncalves_oficial.png" style="​width:​150px;​ height: 150px;"​ /></​div><​br><​br>​
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-<b>Bárbara Oliveira</b> has a BSc in Biology ​and a MSc in Molecular ​Biology and Genetics from Faculdade ​de CiênciasBárbara ​joined the Flow Cytometry ​Facility ​in March 2017, after studying ​the dynamics of circulating coelomocytes during starfish regeneration ​at ITQB in collaboration with the Flow Cytometry Core Facility ​of IGC, headed by Rui GardnerBárbara was the first in the current team to attended the BD FACSAria Advanced Operator Course ​in Belgium.<br>+<b>Rute Gonçalves</b> has a BSc in Biochemistry ​and a MSc in Molecular Genetics ​and Biomedicine ​from Universidade Nova de LisboaRute joined the Flow Cytometry ​Unit in December 2018, after working at the Immunobiology and Pathogenesis Lab at CEDOC, under the supervision ​of DraHelena Soares, studying ​the main TLR4 signaling pathways ​in human T cells. She was also responsible for CEDOC’s Flow Cytometry Facility from April 2018 to October 2018, providing cell sorting services, supporting researchers ​in cell analyzers as well as software and data analysis and of course performing all the daily equipment maintenance and quality control. <br>
 <img src="​https://​imm.medicina.ulisboa.pt/​facility/​flowcytometry/​lib/​exe/​fetch.php?​media=mail_icon.png"​ width=20> ​ <img src="​https://​imm.medicina.ulisboa.pt/​facility/​flowcytometry/​lib/​exe/​fetch.php?​media=mail_icon.png"​ width=20> ​
- <​i>​barbara.oliveira@medicina.ulisboa.pt</​i>​ + <​i>​rute.goncalves@medicina.ulisboa.pt</​i>​
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-<div style="​width:​ 150px; height: 150px; overflow: hidden; position: relative;"><​img src="/​facility/​flowcytometry/​lib/​exe/​fetch.php?​media=diana_macedo.png"​ style="​width:​150px;​ height: 150px;"​ /></​div><​br><​br>​ +
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-<​b>​Diana Macedo</​b>​ has a MSc in Neurosciences and a PhD in Biochemistry. Diana joined the Flow Cytometry Facility in November 2017 after postdoctoral research at the Max Planck Institute for Biology of Ageing, where she developed high-throughput flow cytometry methods to study autophagy. Diana was trained by Kat Folz-Donahue at the Flow Cytometry Facility at MPI-AGE and has trained herself students and colleagues, besides participating in different research projects that resulted in 10 published articles. She is our FlowJo expert.<​br>​ +
-<img src="​https://​imm.medicina.ulisboa.pt/​facility/​flowcytometry/​lib/​exe/​fetch.php?​media=mail_icon.png"​ width=20>​  +
- <​i>​dianamacedo@medicina.ulisboa.pt</​i>​+
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 ===== How to use the service ===== ===== How to use the service =====
  
-All new users must contact the Flow Cytometry ​Facility ​to get proper training in using the systems and know the usage rules. Only after training will a user gain access to the Flow Cytometry room and to the online booking system. To get access to the cell sorters online booking, users must contact the Flow Cytometry staff. +All new users must contact the Flow Cytometry ​Unit to get proper training in using the systems and know the usage rules. Only after training will a user gain access to the Flow Cytometry room and to the online booking system. To get access to the cell sorters online booking, users must contact the Flow Cytometry staff. 
-Please read our policies and guidelines [[https://​imm.medicina.ulisboa.pt/​files/6714/6348/2676/SOP.UCF.004_-_UCF_IMM_USER_GUIDELINES.pdf|here]].+Please read our policies and guidelines [[https://​imm.medicina.ulisboa.pt/​facility/flowcytometry/lib/exe/fetch.php?​media=sop.ucf.004_-_ucf_imm_user_guidelines.pdf|here]]. 
 + 
 +{{:​UCF_users_over_years.png?​400|}} {{:​UCF_usage_hours.png?​400|}} \\
  
 {{red-arrow-pointing-right.jpg|}} **Are you a** [[newuser|{{new_user.jpg?​0x30|}}]] {{red-arrow-pointing-right.jpg|}} **Are you a** [[newuser|{{new_user.jpg?​0x30|}}]]
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 ===== Contacts ===== ===== Contacts =====
  
-Flow Cytometry ​Facility\\+Flow Cytometry ​Unit\\
 **imm-flowcytometry@medicina.ulisboa.pt** **imm-flowcytometry@medicina.ulisboa.pt**
  
about.txt · Last modified: 2024/02/28 22:29 by flowcytometry