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about [2018/02/27 14:53] flowcytometry |
about [2021/11/17 18:50] flowcytometry |
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- | ====== About the Flow Cytometry Facility ====== | + | ====== About the Flow Cytometry Unit ====== |
- | The Flow Cytometry Facility provides a state of the art flow cytometry service to iMM Lisboa researchers as well as external groups from other research institutes. We aim at training researchers in the adequate use of the instruments and also on flow cytometry principles, experiment planning, as well as data analysis and interpretation. The Flow Cytometry Facility also provides a cell sorting service using two High Speed Cell Sorters (BD FACSAria III and BD FACSAria IIu) operated by dedicated staff. | + | {{:flow_wiki_intro.gif?300 |}} \\ \\ \\ \\ \\ \\ The Flow Cytometry Unit provides a state of the art flow cytometry service to iMM researchers as well as external groups from other research institutes. We aim at training researchers in the adequate use of the instruments and also on flow cytometry principles, experiment planning, as well as data analysis and interpretation. The Flow Cytometry Facility also provides a cell sorting service using two High Speed Cell Sorters (BD FACSAria III and BD FACSAria IIu) operated by dedicated staff. |
===== Team ===== | ===== Team ===== | ||
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- | {{::flow_cytometry_team.jpg?650|}} | ||
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- | <div align="center"><b>Head of Facility</b></div> | + | <div align="center"><b>Head of Unit</b></div> |
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- | <img src="/facility/flowcytometry/lib/exe/fetch.php?media=jose_rino.png" width=360></a><br><br> | + | <div style="width: 150px; height: 150px; overflow: hidden; position: relative;"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=marianafoto.png" style="width:150px; height: 150px;" /></div><br><br> |
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<br> | <br> | ||
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- | <b>José Rino</b> has a <i>Licenciatura</i> in Technological Physics Engineering (Instituto Superior Técnico) and a PhD in Biophysics (Faculdade de Ciências). José was appointed Head of the Flow Cytometry Facility in October 2016. He is also Head of the Bioimaging Facility at iMM. <a href="https://imm.medicina.ulisboa.pt/facility/bioimaging/doku.php?id=facility_bioimaging_doku.php&id=rino_cv">More information</a>.<br> | + | <b>Mariana Fernandes</b> has a MSc degree in Biomedical Sciences from the Instituto de Higiene e Medicina Tropical at the Universidade Nova de Lisboa. She had her first contact with flow cytometry while working as a research technician and during her Master’s. During this time, she was involved in several projects focused in characterizing the internalization of classic and new antileishmanial drugs or compounds by macrophages infected with Leishmania spp. Her first flow cytometry work began in 2015, as a technician in the Flow Cytometry Unit at iMM. Afterwards, she moved to the IGC Flow Cytometry Facility as a Research Support Specialist, where she worked for several years. With the obtained experience, she embraced a new challenge, going to the Institute of Microbiology, Infectious Diseases and Immunology at Charité – Universitätsmedizin in Berlin, where she set up and managed the new Benjamin Franklin Flow Cytometry Facility. In 2021, she was appointed as Head of Flow Cytometry Unit at Instituto de Medicina Molecular João Lobo Antunes (iMM).<br> |
<img src="https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=mail_icon.png" width=20> | <img src="https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=mail_icon.png" width=20> | ||
- | <i>joserino@medicina.ulisboa.pt</i> | + | <i>mariana.fernandes@medicina.ulisboa.pt</i> |
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<div align="center"><b>Technical Support</b></div> | <div align="center"><b>Technical Support</b></div> | ||
<br> | <br> | ||
- | <img src="/facility/flowcytometry/lib/exe/fetch.php?media=andreia_santos.png" width=380></a><br><br> | + | <div style="width: 150px; height: 150px; overflow: hidden; position: relative;"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=andreiafoto.png" style="width:150px; height: 150px;" /></div><br><br> |
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- | <b>Andreia Santos</b> has a BSc in Biology and a MSc in Genetic and Molecular Biology from Faculdade de Ciências. Andreia joined the Flow Cytometry Facility in September 2017 after working at Ana Espada de Sousa’s lab on human T-cell development, namely the role of IL-4 in the lineage plasticity of human CD4 single positive thymocytes.<br> | + | <b>Andreia Santos</b> has a BSc in Biology and a MSc in Genetic and Molecular Biology from Faculdade de Ciências, Universidade de Lisboa. Andreia joined the Flow Cytometry Unit in September 2017 after working at Ana Espada de Sousa’s lab on human T-cell development, namely the role of IL-4 in the lineage plasticity of human CD4 single positive thymocytes.<br> |
<img src="https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=mail_icon.png" width=20> | <img src="https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=mail_icon.png" width=20> | ||
<i>assantos@medicina.ulisboa.pt</i> | <i>assantos@medicina.ulisboa.pt</i> | ||
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- | <img src="/facility/flowcytometry/lib/exe/fetch.php?media=barbara_oliveira.png" width=380></a><br><br> | + | <div style="width: 150px; height: 150px; overflow: hidden; position: relative;"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=margaridafoto.png" style="width:150px; height: 150px;" /></div><br><br> |
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- | <b>Bárbara Oliveira</b> has a BSc in Biology and a MSc in Molecular Biology and Genetics from Faculdade de Ciências. Bárbara joined the Flow Cytometry Facility in March 2017, after studying the dynamics of circulating coelomocytes during starfish regeneration at ITQB in collaboration with the Flow Cytometry Core Facility of IGC, headed by Rui Gardner. Bárbara was the first in the current team to attended the BD FACSAria Advanced Operator Course in Belgium.<br> | + | <b>Margarida Araújo</b> has a BSc in Biochemistry and a MSc in Molecular Genetics and Biomedicine from Universidade Nova de Lisboa. Rute joined the Flow Cytometry Unit in December 2018, after working at the Immunobiology and Pathogenesis Lab at CEDOC, under the supervision of Dra. Helena Soares. She was also responsible for CEDOC’s Flow Cytometry Facility from April 2018 to October 2018, providing cell sorting services, supporting researchers in cell analyzers as well as software and data analysis and performing all the daily equipment maintenance and quality control. <br> |
<img src="https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=mail_icon.png" width=20> | <img src="https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=mail_icon.png" width=20> | ||
- | <i>barbara.oliveira@medicina.ulisboa.pt</i> | + | <i>rute.goncalves@medicina.ulisboa.pt</i> |
</td> | </td> | ||
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- | <img src="/facility/flowcytometry/lib/exe/fetch.php?media=diana_macedo.png" width=680></a><br><br> | + | <div style="width: 150px; height: 150px; overflow: hidden; position: relative;"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=rutefoto.png" style="width:150px; height: 150px;" /></div><br><br> |
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<span style="line-height:1.8"> | <span style="line-height:1.8"> | ||
- | <b>Diana Macedo</b> has a MSc in Neurosciences and a PhD in Biochemistry. Diana joined the Flow Cytometry Facility in November 2017 after postdoctoral research at the Max Planck Institute for Biology of Ageing, where she developed high-throughput flow cytometry methods to study autophagy. Diana was trained by Kat Folz-Donahue at the Flow Cytometry Facility at MPI-AGE and has trained herself students and colleagues, besides participating in different research projects that resulted in 10 published articles. She is our FlowJo expert.<br> | + | <b>Rute Gonçalves</b> has a BSc in Biochemistry and a MSc in Molecular Genetics and Biomedicine from Universidade Nova de Lisboa. Rute joined the Flow Cytometry Unit in December 2018, after working at the Immunobiology and Pathogenesis Lab at CEDOC, under the supervision of Dra. Helena Soares. She was also responsible for CEDOC’s Flow Cytometry Facility from April 2018 to October 2018, providing cell sorting services, supporting researchers in cell analyzers as well as software and data analysis and performing all the daily equipment maintenance and quality control. <br> |
<img src="https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=mail_icon.png" width=20> | <img src="https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=mail_icon.png" width=20> | ||
- | <i>dianamacedo@medicina.ulisboa.pt</i> | + | <i>rute.goncalves@medicina.ulisboa.pt</i> |
</td> | </td> | ||
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===== Prices ===== | ===== Prices ===== | ||
- | Users are billed for the actual time of usage. [[https://imm.medicina.ulisboa.pt/intranet/booking/select_resource.php?v=1|Online booking]] is mandatory for each usage session. **Cancellations and no shows will be charged extra**, please check the [[usage_policy|usage rules]] for more details. | + | Users are billed for the actual time of usage. [[https://imm.agendo.science|Online booking]] is mandatory for each usage session. **Cancellations and no shows will be charged extra**, please check the [[usage_policy|usage rules]] for more details. |
{{red-arrow-pointing-right.jpg|}} **Check our** [[billing|{{charge_rates.jpg?0x30|}}]] | {{red-arrow-pointing-right.jpg|}} **Check our** [[billing|{{charge_rates.jpg?0x30|}}]] | ||
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===== How to use the service ===== | ===== How to use the service ===== | ||
- | All new users must contact the Flow Cytometry Facility to get proper training in using the systems and know the usage rules. Only after training will a user gain access to the Flow Cytometry room and to the online booking system. To get access to the cell sorters online booking, users must contact the Flow Cytometry staff. | + | All new users must contact the Flow Cytometry Unit to get proper training in using the systems and know the usage rules. Only after training will a user gain access to the Flow Cytometry room and to the online booking system. To get access to the cell sorters online booking, users must contact the Flow Cytometry staff. |
- | Please read our policies and guidelines [[https://imm.medicina.ulisboa.pt/files/6714/6348/2676/SOP.UCF.004_-_UCF_IMM_USER_GUIDELINES.pdf|here]]. | + | Please read our policies and guidelines [[https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.ucf.004_-_ucf_imm_user_guidelines.pdf|here]]. |
+ | |||
+ | {{:UCF_users_over_years.png?400|}} {{:UCF_usage_hours.png?400|}} \\ | ||
{{red-arrow-pointing-right.jpg|}} **Are you a** [[newuser|{{new_user.jpg?0x30|}}]] | {{red-arrow-pointing-right.jpg|}} **Are you a** [[newuser|{{new_user.jpg?0x30|}}]] | ||
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===== Contacts ===== | ===== Contacts ===== | ||
- | Flow Cytometry Facility\\ | + | Flow Cytometry Unit\\ |
**imm-flowcytometry@medicina.ulisboa.pt** | **imm-flowcytometry@medicina.ulisboa.pt** | ||
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{{telephone_icon.gif?20|}}Internal phone extension: 47222 / 47224 | {{telephone_icon.gif?20|}}Internal phone extension: 47222 / 47224 | ||
+ | |||
+ | {{::TEAM PHOTO 2021.jpg?500|}} \\ | ||
+ | UCF Team in 2021. Photo: Gonçalo Ribeiro | ||
---- | ---- | ||
[[start|Back to the Flow Cytometry Wiki]] | [[start|Back to the Flow Cytometry Wiki]] |