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====== About the Flow Cytometry Facility ====== | ====== About the Flow Cytometry Facility ====== | ||
- | The Flow Cytometry Facility provides a state of the art flow cytometry service to iMM Lisboa researchers as well as external groups from other research institutes. We aim at training researchers in the adequate use of the instruments and also on flow cytometry principles, experiment planning, as well as data analysis and interpretation. The Flow Cytometry Facility also provides a cell sorting service using two High Speed Cell Sorters (BD FACSAria III and BD FACSAria IIu) operated by dedicated staff. | + | The Flow Cytometry Facility provides a state of the art flow cytometry service to iMM researchers as well as external groups from other research institutes. We aim at training researchers in the adequate use of the instruments and also on flow cytometry principles, experiment planning, as well as data analysis and interpretation. The Flow Cytometry Facility also provides a cell sorting service using two High Speed Cell Sorters (BD FACSAria III and BD FACSAria IIu) operated by dedicated staff. |
===== Team ===== | ===== Team ===== | ||
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- | {{::flow_cytometry_team.jpg?650|}} | ||
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<div align="center"><b>Head of Facility</b></div> | <div align="center"><b>Head of Facility</b></div> | ||
<br> | <br> | ||
- | <img src="/facility/flowcytometry/lib/exe/fetch.php?media=jose_rino.png" width=360></a><br><br> | + | <div style="width: 150px; height: 150px; overflow: hidden; position: relative;"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=jose_rino.png" style="width:150px; height: 150px;" /></div><br><br> |
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<div align="center"><b>Technical Support</b></div> | <div align="center"><b>Technical Support</b></div> | ||
<br> | <br> | ||
- | <img src="/facility/flowcytometry/lib/exe/fetch.php?media=andreia_santos.png" width=380></a><br><br> | + | <div style="width: 150px; height: 150px; overflow: hidden; position: relative;"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=andreia_santos.png" style="width:150px; height: 150px;" /></div><br><br> |
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<br> | <br> | ||
<br> | <br> | ||
- | <b>Andreia Santos</b> has a BSc in Biology and a MSc in Genetic and Molecular Biology from Faculdade de Ciências. Andreia joined the Flow Cytometry Facility in September 2017 after working at Ana Espada de Sousa’s lab on human T-cell development, namely the role of IL-4 in the lineage plasticity of human CD4 single positive thymocytes.<br> | + | <b>Andreia Santos</b> has a BSc in Biology and a MSc in Genetic and Molecular Biology from Faculdade de Ciências, Universidade de Lisboa. Andreia joined the Flow Cytometry Facility in September 2017 after working at Ana Espada de Sousa’s lab on human T-cell development, namely the role of IL-4 in the lineage plasticity of human CD4 single positive thymocytes.<br> |
<img src="https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=mail_icon.png" width=20> | <img src="https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=mail_icon.png" width=20> | ||
<i>assantos@medicina.ulisboa.pt</i> | <i>assantos@medicina.ulisboa.pt</i> | ||
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- | <img src="/facility/flowcytometry/lib/exe/fetch.php?media=barbara_oliveira.png" width=380></a><br><br> | + | <div style="width: 150px; height: 150px; overflow: hidden; position: relative;"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=rute_goncalves_oficial.png" style="width:150px; height: 150px;" /></div><br><br> |
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- | <b>Bárbara Oliveira</b> has a BSc in Biology and a MSc in Molecular Biology and Genetics from Faculdade de Ciências. Bárbara joined the Flow Cytometry Facility in March 2017, after studying the dynamics of circulating coelomocytes during starfish regeneration at ITQB in collaboration with the Flow Cytometry Core Facility of IGC, headed by Rui Gardner. Bárbara was the first in the current team to attended the BD FACSAria Advanced Operator Course in Belgium.<br> | + | <b>Rute Gonçalves</b> has a BSc in Biochemistry and a MSc in Molecular Genetics and Biomedicine from Universidade Nova de Lisboa. Rute joined the Flow Cytometry Facility in December 2018, after working at the Immunobiology and Pathogenesis Lab at CEDOC, under the supervision of Dra. Helena Soares, studying the main TLR4 signaling pathways in human T cells. She was also responsible for CEDOC’s Flow Cytometry Facility from April 2018 to October 2018, providing cell sorting services, supporting researchers in cell analyzers as well as software and data analysis and of course performing all the daily equipment maintenance and quality control. <br> |
<img src="https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=mail_icon.png" width=20> | <img src="https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=mail_icon.png" width=20> | ||
- | <i>barbara.oliveira@medicina.ulisboa.pt</i> | + | <i>rute.goncalves@medicina.ulisboa.pt</i> |
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- | <img src="/facility/flowcytometry/lib/exe/fetch.php?media=diana_macedo.png" width=680></a><br><br> | + | <div style="width: 150px; height: 150px; overflow: hidden; position: relative;"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=joana_monteiro.png" style="width:150px; height: 150px;" /></div><br><br> |
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<span style="line-height:1.8"> | <span style="line-height:1.8"> | ||
- | <b>Diana Macedo</b> has a MSc in Neurosciences and a PhD in Biochemistry. Diana joined the Flow Cytometry Facility in November 2017 after postdoctoral research at the Max Planck Institute for Biology of Ageing, where she developed high-throughput flow cytometry methods to study autophagy. Diana was trained by Kat Folz-Donahue at the Flow Cytometry Facility at MPI-AGE and has trained herself students and colleagues, besides participating in different research projects that resulted in 10 published articles. She is our FlowJo expert.<br> | + | <b>Joana Monteiro</b> has a <i>Licenciatura</i> in Biochemistry from the Faculdade de Ciências, Universidade de Lisboa. Joana joined the Flow Cytometry Facility in June 2019, after working at the startup Lumisense and before that studying stem cells and metastasis in the Erasmus Medical Center Rotterdam, in the group of Prof. Riccardo Fodde. Joana has already attended a BD Course for basic operator of the FACSAria.<br> |
<img src="https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=mail_icon.png" width=20> | <img src="https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=mail_icon.png" width=20> | ||
- | <i>dianamacedo@medicina.ulisboa.pt</i> | + | <i>joana.monteiro@medicina.ulisboa.pt</i> |
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All new users must contact the Flow Cytometry Facility to get proper training in using the systems and know the usage rules. Only after training will a user gain access to the Flow Cytometry room and to the online booking system. To get access to the cell sorters online booking, users must contact the Flow Cytometry staff. | All new users must contact the Flow Cytometry Facility to get proper training in using the systems and know the usage rules. Only after training will a user gain access to the Flow Cytometry room and to the online booking system. To get access to the cell sorters online booking, users must contact the Flow Cytometry staff. | ||
Please read our policies and guidelines [[https://imm.medicina.ulisboa.pt/files/6714/6348/2676/SOP.UCF.004_-_UCF_IMM_USER_GUIDELINES.pdf|here]]. | Please read our policies and guidelines [[https://imm.medicina.ulisboa.pt/files/6714/6348/2676/SOP.UCF.004_-_UCF_IMM_USER_GUIDELINES.pdf|here]]. | ||
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+ | {{:UCF_users_over_years.png?400|}} {{:UCF_usage_hours.png?400|}} \\ | ||
{{red-arrow-pointing-right.jpg|}} **Are you a** [[newuser|{{new_user.jpg?0x30|}}]] | {{red-arrow-pointing-right.jpg|}} **Are you a** [[newuser|{{new_user.jpg?0x30|}}]] |