GIMM Bioimaging Wiki

3i_marianas_sdc

2026-04-17T12:08:13+02:00

System overview The 3i Marianas SDC spinning disk confocal microscope is a fast imaging system which provides a trade-off between confocality, resolution and speed. It is an inverted microscope ideal for live cell applications which require fast acquisition speeds rather than high resolution images. The scanning unit achieves confocality by directing light through a spinning disk with many small pinholes. Images are then acquired with a sensitive EMCCD which allows for very small exposure time…

3i_marianas_sdc_quality

2022-02-12T15:46:37+02:00

3i Marianas SDC Quality Control 3i_marianas_sdc Click here to get info on the 3i Marianas SDC PSFs Illumination ---------- Back to Quality Control

3i_marianas_sdc_usage

2020-03-03T20:58:02+02:00

3i Marianas SDC Usage Statistics 3i_marianas_sdc Click here to get info on the 3i Marianas SDC ---------- Back to Statistics

about

2026-03-19T16:15:08+02:00

About the Bioimaging Platform The Bioimaging Platform offers state-of-the-art personalized support in all steps of research done with Light Microscopy at GIMM, housing 29 systems including point-scanning confocal, spinning disk confocal, multiphoton, light sheet, widefield, slide scanning, and advanced image analysis workstations.

agilent_cytation_5

2026-03-02T16:39:26+02:00

Microscope overview The Cytation 5 Cell Imaging Multimode Reader is a fully automated plate and slide reader equipped with an inverted widefield configuration. This unit is capable of imaging from 4-40x with fluorescence and/or brightfield (mono or RGB). It is equipped with three different excitation LEDs and filter sets (there is a fourth slot which is mainly used for hardware-based autofocus, but which can be replaced by a 4th fluorescence channel upon request).

amira

2026-04-07T18:46:37+02:00

System overview This workstation has Amira v2020 for 3D image processing and analysis and the Gen5 software (for processing+analysing Cytation5 datasets). It can also be booked for processing demanding FIJI, QuPath and Ilastik operations, and other open-source tools (eg, AI/ML workflows). If you are not familiar with Amira you can find excellent tutorials here:

andor_faq

2016-04-17T01:36:50+02:00

Andor Revolution FAQ Q. Why do I see a striped pattern in my image? A. If you see strips in your image then the spinning disk rotation may not be synchronized with the camera exposure time. Mismatches between the camera exposure time and spinning disk rotation speed are usually not a problem for long exposure times (> 100 ms) but they can seriously impair image quality when exposure times are down to less than 100 ms. A mismatch occurs whenever the exposure time is not an integral multiple of…

andor_revolution

2021-01-17T04:48:57+02:00

System overview The Andor Revolution spinning disk confocal microscope is a fast imaging system which provides a trade-off between confocality, resolution and speed. It is an inverted microscope ideal for live cell applications which require fast acquisition speeds rather than high resolution images. The scanning unit achieves confocality by directing light through a

andor_usage

2020-03-03T21:22:08+02:00

Andor Revolution Usage Statistics Click here to get info on the Andor Revolution Microscope is no longer in use ---------- Back to Statistics

andor_w1

2025-07-16T16:36:41+02:00

Microscope overview The Andor Spinning W1 system is a spinning disk laser confocal microscope with a sensitive EMCCD camera suitable for fast in-vivo imaging. It's one of the fastest and most sensitive systems at the facility. The W1 also has installed microinjector and micromanipulation units and is ready for microfuildics experiments. Note that this system does not have widefield fluorescence, so samples can only be previewed in brightfield mode.

bambulab_x1e

2026-03-17T14:35:26+02:00

Disclaimer The Bioimaging Platform does not provide regular trainings in this system, but biannually 3D printing courses. Next course date: April 14th 2026 We also do not provide 3D designing as a service. You can contact Jorge Carvalho (ITQB) for this service.

barbosa_cv

2025-01-09T17:09:33+02:00

Beatriz Barbosa, MSc Publishing Name: Ana Beatriz Barbosa Ciência-ID: 091C-17D3-85F6 Nationality: Portuguese E-mail address: beatriz.barbosa@gimm.pt ---------- Research History Beatriz Barbosa has a BSc in Biology from Faculdade de Ciências da Universidade de Lisboa, with the specialized branch of Evolutionary and Developmental Biology (2019), and a MSc in Biomedical Research from the Faculdade de Medicina da Universidade de Lisboa (2024).

big_guy

2021-04-09T16:46:27+02:00

System overview Big Guy is a dual boot 64 bit Mac Pro running either Mac OS X Snow Leopard or Windows 10, equipped with: * 2 Quad-core processors (that's 8 processors) @ 2.4 GHz * 16 Gb RAM memory * 2 Tb disk space (1 Tb for each operating system)

big_guy_usage

2020-03-03T21:19:57+02:00

Big Guy Usage Statistics big_guy Click here to get info on the Big Guy ---------- Back to Statistics

billing

2026-02-18T12:57:42+02:00

Microscope Charge Rates for 2026 (€/hour) Lisbon Site Equipment Campus Academic Industry IVIS Lumina LT 0.28 0.65 1.16 Leica DM2500 0.44 1.00 1.79 ZEISS Axio Zoom.V16 0.97 2.19 3.95 ZEISS Axiovert 200M 0.23 0.52 0.94 Nikon Eclipse Ti 0.85 1.93 3.47

blaze

2026-02-17T17:13:58+02:00

Microscope overview The UltraMicroscope Blaze is a lightsheet for large cleared samples system with dual sided illumination, capable of using three beams per side. This allows for uniform imaging with minimal shadows (in good sample clearing conditions). Equipped with three objectives (1.1x, 4x and 12x) and internal magnifications of 0.6x, 1x, 1.66x and 2.5x, this system is capable of going from whole organs to single cell level. There are 4 laser lines in the Blaze, 488, 561, 640 and 785 nm, a…

booking_warning

2020-05-19T20:22:29+02:00

* Booking a slot until May 31st? → Please use the old booking system * Booking a slot from June 1st onwards? → Please use the new Agendo booking system ---------- Back to the Bioimaging Wiki

brainstorm

2023-08-06T13:33:22+02:00

Location: no longer available username: immuser password: !immuser System overview Brainstorm is a linux PC workstation featuring: * Huygens Essentials and Huygens Script prepared for Widefield and Confocal deconvolution (blind and non-blind).

carvalho_cv

2025-01-23T15:57:05+02:00

Jorge Carvalho, PhD Publishing Name: Jorge de-Carvalho Ciência-ID: 7A10-A355-2334 Nationality: Portuguese E-mail address: jorge.carvalho@gimm.pt ---------- Research History Jorge Carvalho has vast, cross-disciplinary experience in the fields of biology, engineering, chemistry, physics and materials science. During 14 years of researc…

chronos

2026-04-17T01:17:41+02:00

System overview Chronos is a 64-bit PC running Windows 10, equipped with: * 2x Intel EMR 6534 2P 8C Processors @ 3.9GHz * 256 Gb RAM memory * System disk: 960GB Samsung PM9A3 NVMe PCIe * Data Storage: 7 Tb * Data Processing: 7 Tb (2x Samsung PM893 3.84 TB SATA 6Gb/s as RAID 0)

chronos_usage

2023-09-01T22:27:51+02:00

Chronos Usage Statistics chronos Click here to get info on the Chronos ---------- Back to Statistics

colossus

2024-01-18T01:23:15+02:00

System overview Colossus is a 64-bit PC running Windows 10, equipped with: * 4 Quad-core processors (16 processors) @ 3.0GHz * 128 GB RAM memory * 512 GB SSD + 10 TB disk space (2 x 5 TB disks for data storage) * 4 GB Radeon R9 290X OC (graphic card)

colossus_usage

2020-03-03T21:20:25+02:00

Colossus Usage Statistics colossus Click here to get info on the Colossus ---------- Back to Statistics

confocal_practical_course

2019-08-01T18:18:32+02:00

Fluorescence and Confocal Microscopy Practical Course - 2012 This practical course aims at providing the students with a solid base in fluorescence and confocal microscopy for histology applications. The following topics will be covered: performing fluorescence microscopy histology, brightfield and fluorescence microscopy, fluorescent probes, advanced fluorescence techniques (FRAP, FRET), practical usage of widefield fluorescence and confocal microscopes, digital image processing.

courses

2026-02-26T10:37:43+02:00

μCourses REGISTER HERE The Bioimaging μCourses address basic topics on bioimaging technologies and techniques, with a focus on those available at GIMM or PPBI/EuroBioimaging. This will complement the one-on-one training we provide daily, and is a forum where you can learn more about the basics of optics, fluorescence, illumination, instrumentation, super-resolution, mesoscopy, 3D microscopy, confocal, light-sheet, image quality, sample preparation, analysis workflows, etc

coutinho_cv

2025-01-06T16:41:14+02:00

Diogo Coutinho, MSc Publishing Name: Diogo Coutinho Ciência-ID: C614-76DA-8A3A Nationality: Portuguese E-mail address: diogo.coutinho@gimm.pt ---------- Research History Diogo Coutinho has a BSc in Biochemistry from Universidade de Évora (2019) and a MSc in Oncobiology by the Faculdade de Medicina da Universidade de Lisboa (2024).

cytation_5_quality

2025-10-31T16:13:51+02:00

[Oeiras] Agilent Cytation 5 agilent_cytation_5 Click here to get info on the [Oeiras] Agilent Cytation 5 Illumination ---------- Back to Quality Control

deltavision

2025-12-12T11:03:48+02:00

Microscope overview SIM microscopy is one of the imaging techniques that can overcome the limits of resolution of widefield fluorescence microscopes (~200nm), ie, it allows “sub-diffraction” imaging. By using patterned (“structured”) illumination, higher order spatial information is obtained that can then be extracted by computational algorithms in Fourier space. A typical DM OMX-SIM image is a reconstruction of 15 images, where the illumination pattern changes in angle (3x) and phase (5x). SIM…

dragonfly

2026-01-29T17:26:38+02:00

Microscope overview Spinning disk confocal microscopy (SDC) is a technique well suited for observation of cells and organoids. Compared to conventional widefield microscopy, SDC provides instant optical sectioning, allowing the imaging of cells and shallow tissues (typically a maximum of a few tens of micrometres deep) in 3D. Compared to laser scanning confocal microscopes (such as Leica Stellaris, ZEISS Airyscan), the Dragonfly SDC provides much faster acquisition (up to 400 fps, with limit…

dragonfly_quality

2025-10-31T16:23:05+02:00

[Oeiras] Andor Dragonfly SDC Quality Control dragonfly Click here to get info on the [Oeiras] Andor Dragonfly SDC PSFs Illumination ---------- Back to Quality Control

gaby_cv

2025-06-18T00:41:27+02:00

Gaby Martins, PhD Publishing Name: Gabriel G Martins Ciência-ID: 3216-B514-FE35 Nationality: Portuguese E-mail address: gaby@gimm.pt ---------- Research History Gabriel (“Gaby”) Martins completed his PhD in Anatomy & Cell Biology in 20…

hmsp_workshop_2010_rate

2020-08-03T17:40:05+02:00

Workshop: Quantitative Light Microscopy in Living Cells How good was it? Student's feedback: 1 (bad); 2 (mediocre); 3 (sufficient); 4 (good); 5 (excellent) ---------- Back to the Bioimaging Courses

huygens

2025-01-13T16:47:12+02:00

System overview This workstation has Huygens v23.10.0p2 for image deconvolution and aberration correction. It can also be booked for processing demanding FIJI, QuPath and Ilastik operations, and other open-source tools (eg, AI/ML workflows). Workstation specs (2025)

imager_quality

2025-10-31T16:16:06+02:00

[Oeiras] ZEISS Axio Imager with Apotome 2 zeiss_apotome Click here to get info on the [Oeiras] ZEISS Axio Imager with Apotome 2 PSFs Illumination ---------- Back to Quality Control

imaris

2026-04-07T18:46:45+02:00

System overview This workstation has Imaris v10.2.0 for image 3D visualization and analysis workflows. It can also be booked for processing demanding FIJI, QuPath and Ilastik operations, and other open-source tools (eg, AI/ML workflows). Workstation specs (2025)

inserm

2020-08-03T16:24:01+02:00

INSERM Workshop - Phase II - Practical Course Coordinator: José Rino (IMM) Date: September 7-10, 2011 Location: Instituto de Medicina Molecular, Lisbon Description The practical component of the Workshop will consist of two modules designed to complement each other:

inserm_2011_rate

2020-08-03T16:25:38+02:00

Phase II (Lisbon) How good was it? Student's feedback: 1 (bad); 2 (mediocre); 3 (sufficient); 4 (good); 5 (excellent) ---------- Back to the Bioimaging Courses

ivis_lumina

2025-11-22T19:59:45+02:00

System overview The IVIS Lumina fluorescence/bioluminescence imaging system provides high sensitivity, ease of use, and flexibility to image fluorescent and/or bioluminescent reporters both in vivo and in vitro. The system includes a highly sensitive CCD camera, a light-tight imaging chamber with complete computer automation, and the Living Image software package for image acquisition and analysis.

ivis_lumina_lt

2026-02-05T11:29:40+02:00

System overview The IVIS Lumina LT fluorescence/bioluminescence imaging system is equipped with 2D bioluminescence, fluorescence, and radioisoptic (Cerenkov) imaging capabilities, providing high sensitivity, ease of use, and flexibility to image fluorescent and/or bioluminescent reporters both

ivis_lumina_quality

2024-10-07T17:46:36+02:00

IVIS Lumina Quality Control ivis_lumina Click here to get info on the IVIS Lumina Detection ---------- Back to Quality Control

ivis_lumina_repair

2016-04-09T04:18:49+02:00

IVIS Lumina Repair History * 30/04/14 - The platform motor problem is fixed, the system is working again * 25/04/14 - The platform motor is malfunctioning, it is not possible to adjust the platform height ---------- Click here to get info on the IVIS Lumina

ivis_lumina_usage

2020-03-03T21:19:28+02:00

IVIS Lumina Usage Statistics ivis_lumina Click here to get info on the IVIS Lumina ---------- Back to Statistics

kiosk

2025-11-18T17:59:26+02:00

kiosk_lisbon

2026-04-17T14:37:37+02:00

kiosk_oeiras

2026-04-17T14:44:52+02:00

leica_dm2500

2026-02-17T17:49:24+02:00

Microscope overview The Leica DM2500 is an upright brightfield microscope equipped with a color camera (Leica DFC450) for brightfield and differential interference contrast (DIC) imaging. Data files older than 3 months will be automatically deleted on this system, please copy your data to the GIMM server using the desktop link.

leica_dm2500_repair

2016-04-09T04:07:09+02:00

Leica DM2500 Repair History * 11/07/2014 - A new switching power supply has been installed, the system is back to normal operation * 19/05/2014 - The switching power supply is malfunctioning, the system is offline ---------- Click here to get info on the Leica DM2500

leica_dm2500_usage

2020-03-03T21:18:56+02:00

Leica DM2500 Usage Statistics leica_dm2500 Click here to get info on the Leica DM2500 ---------- Back to Statistics

leica_dm5000b

2023-08-06T13:30:14+02:00

Microscope overview The Leica DM5000B is an automated upright widefield fluorescence microscope ideal for checking FISH or other fixed fluorescence slides. The microscope is prepared for brightfield, phase contrast, DIC and polarization contrast techniques (check objectives list for contrast method availability). If you want to image live samples and/or perform time-lapse experiments check out the

leica_dm5000b_quality

2022-02-12T17:39:10+02:00

Leica DM5000B Quality Control leica_dm5000b Click here to get info on the Leica DM5000B Illumination ---------- Back to Quality Control

leica_dm5000b_repair

2016-04-09T04:09:01+02:00

Leica DM5000B Repair History * 16/05/2014 - The fluorescence filter cube turret is malfunctioning * 16/05/2014 - The motorized condenser is malfunctioning: transmitted light settings are not set properly * 16/05/2014 - The Field Diaphragm and Aperture adjustment buttons are not working

leica_dm5000b_usage

2020-03-03T21:16:24+02:00

Leica DM5000B Usage Statistics leica_dm5000b Click here to get info on the Leica DM5000B ---------- Back to Statistics

leica_dmr

2021-01-17T04:49:28+02:00

Microscope overview The Leica DMR is an upright widefield fluorescence microscope equipped with phase contrast objectives and a color camera for brightfield/fluorescence imaging. If its color camera is not sensitive enough for your fluorescent sample try the

leica_dmr_repair

2016-04-17T02:03:05+02:00

Leica DMR Repair History * 02/07/2014 - The malfunctioning computer was replaced by a new one * 30/06/2014 - A new 19“ monitor was installed on the system * 06/06/2014 - The computer is malfunctioning, image acquisition is not possible ----------

leica_dmr_usage

2020-03-03T21:21:09+02:00

Leica DMR Usage Statistics Click here to get info on the Leica DMR Microscope is no longer in use ---------- Back to Statistics

leica_sd_quality

2025-10-31T16:24:42+02:00

[Oeiras] Leica Spinning Disk Confocal Quality Control leica_sdc Click here to get info on the [Oeiras] Leica Spinning Disk Confocal PSFs Illumination ---------- Back to Quality Control

leica_sdc

2026-02-02T01:46:08+02:00

Microscope overview The Leica Spinning Disk Confocal Microscope is a high-performance imaging system designed for dynamic live cell studies, offering a balance between speed, resolution, and confocality. It uses a spinning disk with multiple pinholes to block out-of-focus light, while a sensitive EMCCD Andor

leica_sp8_mp

2025-01-14T16:44:30+02:00

Microscope overview The Leica SP8 MP is a both a confocal and multi-photon microscope able to generate high-resolution three-dimensional images of thick specimens. Multi-photon excitation (most commonly two photon excitation) is particularly advantageous for imaging thicker samples. Rather than exciting the fluorophore with one photon, multi-photon excitation is produced by two or more lower energy photons which can penetrate deeper in the sample. Moreover, contrarily to confocal, the multi-ph…

leica_sp8_mp_quality

2022-02-12T17:15:12+02:00

Leica SP8 MP Quality Control leica_sp8_mp Click here to get info on the Leica SP8 MP PSFs Illumination ---------- Back to Quality Control

leica_sp8_mp_usage

2020-03-03T21:00:43+02:00

Leica SP8 MP Usage Statistics leica_sp8_mp Click here to get info on the Leica SP8 MP ---------- Back to Statistics

light_sheet_workshop_2022

2022-06-20T00:26:30+02:00

* Date: June 7 - 8, 2022 * Location: Instituto de Medicina Molecular João Lobo Antunes * Deadline for registration: June 2nd, 2022 About Light sheet fluorescence microscopy uses a thin sheet of light to excite only fluorophores within the focal volume and orthogonal detection for high-speed 3D imaging of large, optically cleared or water-immersed live samples. This workshop intends to provide a brief introduction to light sheet microscopy and applications and is designed for cell bio…

light_sheet_workshop_rate

2022-07-09T16:39:01+02:00

How good was it? ---------- Back to the Bioimaging Courses

lopes_cv

2025-01-02T20:49:51+02:00

Alexandre Lopes, PhD Publishing Name: Alexandre Lopes Ciência-ID: DE1F-969E-1221 Nationality: Portuguese E-mail address: alexandre.lopes@gimm.pt ---------- Research History Alexandre Lopes has a Licenciatura in Physics and a MSc in Nuclear and Particle Physics from Faculdade de Ciências, Universidade de Lisboa. During his graduate studie…

lsm_510_usage

2020-03-03T21:22:20+02:00

Zeiss LSM 510 Usage Statistics Click here to get info on the Zeiss LSM 510 Microscope is no longer in use ---------- Back to Statistics

maps

2025-07-16T17:06:15+02:00

Where are the microscopes? ---------- Back to the Equipment page

maps_oeiras

2025-07-16T17:12:34+02:00

Where are the microscopes? ---------- Back to the Equipment page

microscopytools

2025-01-14T16:41:57+02:00

Software developed by the Bioimaging Platform * STaQTool - Spot Tracking and Quantification Tool * Track diffraction-limited spots in 2D or 3D time-lapse sequences * Quantify total fluorescence intensity with Gaussian fitting * Detect single events of fluorescence gain and loss

nanocourse_2009

2020-08-03T17:43:34+02:00

BioImaging NanoCourse on Microscopy - 2009 This Nano Course will give you the opportunity to become familiar with both basic microscopy techniques and more advanced 3D microscopy and live imaging during two intensive afternoon sessions. Whether you are a beginning or intermediate microscope user, you will have the chance to learn all the relevant background in fluorescent widefield and confocal microscopy as well as obtain practical information on how to efficiently prepare samples, acquire d…

nanocourse_2009_rate

2020-08-03T17:48:18+02:00

BioImaging NanoCourse on Microscopy - 2009 How good was it? Student's feedback: 1 (bad); 2 (mediocre); 3 (sufficient); 4 (good); 5 (excellent) ---------- Back to the Bioimaging Courses

nanocourse_2011

2020-01-02T01:04:11+02:00

BioImaging NanoCourse on Microscopy, 5 - 6 December 2011 “If motorists drove their cars the way many cell biologists used their confocal microscopes, our highways would be littered with twisted wrecks of automobiles and the mangled corpses of run-over pedestrians. Everyone recognizes that driving a car is a tricky procedure(…) the fact is that a confocal microscope is probably at least as complicated to operate as a car.“ - Wallace Marshall, “Through a pinhole, darkly”

nanocourse_2011_rate

2020-01-02T01:04:23+02:00

BioImaging NanoCourse on Microscopy, 5 - 6 December 2011 How good was it? Student's feedback: 1 (bad); 2 (mediocre); 3 (sufficient); 4 (good); 5 (excellent) ---------- Back to the Bioimaging Courses

nanocourse_2014

2020-01-02T01:03:18+02:00

BioImaging NanoCourse on Microscopy, 15 - 16 May 2014 “If motorists drove their cars the way many cell biologists used their confocal microscopes, our highways would be littered with twisted wrecks of automobiles and the mangled corpses of run-over pedestrians. Everyone recognizes that driving a car is a tricky procedure(…) the fact is that a confocal microscope is probably at least as complicated to operate as a car.“ - Wallace Marshall, “Through a pinhole, darkly”

nanocourse_2014_rate

2020-01-02T01:03:31+02:00

Bioimaging NanoCourse on Microscopy, 15 - 16 May 2014 How good was it? ---------- Back to the Bioimaging Courses

nanocourse_2015

2020-01-02T01:02:42+02:00

BioImaging NanoCourse on Microscopy, 4 - 6 Nov 2015 The Bioimaging Nano Course is an introductory course on Microscopy for beginners or intermediate microscope users. In this course, you will become familiarized with both basic microscopy techniques and more advanced topics such as 3D microscopy, live imaging, FRAP, Photoactivation and FRET, during three afternoon sessions. We will discuss all the relevant background in fluorescent widefield and confocal microscopy and we will also provide pr…

nanocourse_2015_rate

2020-01-02T01:03:02+02:00

Bioimaging NanoCourse on Microscopy, 4 - 6 November 2015 How good was it? ---------- Back to the Bioimaging Courses

nascimento_cv

2025-01-14T16:53:22+02:00

Ana Nascimento, MSc Publishing Name: Ana M. Nascimento Ciência-ID: 5014-2608-2217 Nationality: Portuguese E-mail address: ana.nascimento@gimm.pt ---------- Research History Ana Nascimento has a BSc in Chemistry (2007) and a MSc in Biochemistry (2009) from Faculdade de Ciências, Universidade de Lisboa.

new_systems

2016-04-17T05:54:37+02:00

Systems Upgrade The IMM has recently acquired new microscopy systems. Two of them will replace older microscopes that have lost support from the manufacturer. Here are the details of what is going to happen in the next months. * The Zeiss Axiovert 200M is going to be replaced by a

news_archive

2019-01-18T00:10:09+02:00

* 17/01/19 - And the winners are... check the TOP 5 Users for 2018! * 10/01/19 - Check the Users Evaluation Survey 2018 results 2018 * 11/12/18 - We moved to Room P2-A-32! * 07/07/18 - And the winners are... check the TOP 5 Users for 2017! * 04/04/18 - Check the new Charge Rates for 2018 * 10/01/18 - Check the

newuser

2026-02-20T15:30:31+02:00

How to access our equipment? [Image by Maria Henriques, João Ferreira and José Rino] * Our systems are available to GIMM researchers and external users. Check the equipment hourly fees. * You need to use GIMM's booking system (Agendo) to book our equipment. If you are not registered in Agendo yet, please follow

nikon_eclipse_te2000e

2023-08-06T13:30:34+02:00

Microscope overview The Nikon Eclipse TE2000-E is a fully motorized inverted widefield fluorescence microscope ideal for live-cell imaging applications. It is equipped with a small stage incubator for temperature control and CO2 supply and with Perfect Focus System for hardware focus control during long time-lapse acquisitions. Using either Metamorph or Nikon Elements software you can control the motorized filters, shutters and stage to simultaneously set up multi-color time-lapse imaging exper…

nikon_eclipse_te2000e_usage

2020-03-03T21:10:07+02:00

Nikon Eclipse TE2000-E Usage Statistics nikon_eclipse_te2000e Click here to get info on the Nikon Eclipse TE2000-E ---------- Back to Statistics

nikon_eclipse_ti

2026-03-10T17:31:24+02:00

Microscope overview The Nikon Eclipse Ti is a fully motorized inverted widefield fluorescence microscope ideal for live-cell imaging applications. It is equipped with a small stage incubator for temperature control and CO2 supply, and with Perfect Focus System for hardware focus control during long time-lapse acquisitions. Using NIS-Elements software you can control the motorized filters, shutters and stage to simultaneously set up multi-color time-lapse imaging experiments in multiple stage po…

nikon_eclipse_ti_quality

2022-02-12T17:30:42+02:00

Nikon Eclipse Ti Quality Control nikon_eclipse_ti Click here to get info on the Nikon Eclipse Ti PSFs Illumination ---------- Back to Quality Control

nikon_eclipse_ti_usage

2020-03-03T21:09:12+02:00

Nikon Eclipse Ti Usage Statistics nikon_eclipse_ti Click here to get info on the Nikon Eclipse Ti ---------- Back to Statistics

nikon_htm-hcs

2026-02-19T18:45:21+02:00

Microscope overview The Nikon HTM-HCS is a fully automated microscope capable of high-throughput imaging and screening of samples (eg, Ibidi chamber slides or well-plates); with the help of fully programmable and customizable acquisition protocols.

nikon_htm-hcs_quality

2025-10-31T16:08:42+02:00

[Oeiras] Nikon HTM-HCS Quality Control nikon_htm-hcs Click here to get info on the [Oeiras] Nikon HTM-HCS PSFs Illumination ---------- Back to Quality Control

oeiras_3i_marianas_sdc

2026-01-29T17:20:15+02:00

Microscope overview The 3i Marianas SDC spinning disk confocal microscope is a fast imaging system which provides a combination of confocality, resolution and speed, well suited for the observation of dynamics in living cells and small organisms. The Marianas SDC provides fast acquisition (up to ~100fps, with limited ROI, ~30FPS full frame), and with lower phototoxicity and bleaching. A spinning disk with multiple small pinholes is installed between the light source and specimen to generate po…

oeiras_3i_marianas_sdc_bsl2

2026-01-20T16:18:02+02:00

Microscope overview The 3i Marianas SDC II super-resolution spinning disk confocal microscope is a fast imaging system which provides a trade-off between confocality, resolution and speed, well suited for the observation of dynamics in living cells and small organisms. The Marianas SDC provides fast acquisition (up to ~100fps, with limited ROI, ~30FPS full frame), and with lower phototoxicity and bleaching. A spinning disk with multiple small pinholes is installed between the light source and …

oeiras_lsm_900_quality

2025-10-31T16:26:38+02:00

[Oeiras] ZEISS LSM900 with Airyscan 2 Quality Control zeiss_lsm_900 Click here to get info on the [Oeiras] ZEISS LSM900 with Airyscan 2 PSFs Illumination ---------- Back to Quality Control

oeiras_lsm_980_quality

2025-10-31T16:28:19+02:00

[Oeiras] ZEISS LSM980 with Airyscan 2 Quality Control zeiss_lsm_980_oeiras Click here to get info on the [Oeiras] ZEISS LSM980 with Airyscan 2 PSFs Illumination ---------- Back to Quality Control

oeiras_lumar_quality

2025-10-31T16:34:06+02:00

[Oeiras] ZEISS Stereo Lumar V.12 Quality Control oeiras_zeiss_lumar_v12 Click here to get info on the [Oeiras] ZEISS Stereo Lumar V.12 Illumination ---------- Back to Quality Control

oeiras_marianas_ii_quality

2025-10-31T16:21:44+02:00

[Oeiras] 3i Marianas SDC II + TIRF (BSL2) Quality Control oeiras_3i_marianas_sdc_bsl2 Click here to get info on the [Oeiras] 3i Marianas SDC II + TIRF (BSL2) PSFs Illumination ---------- Back to Quality Control

oeiras_marianas_quality

2025-10-31T16:18:39+02:00

[Oeiras] 3i Marianas SDC Quality Control oeiras_3i_marianas_sdc Click here to get info on the [Oeiras] 3i Marianas SDC PSFs Illumination ---------- Back to Quality Control

oeiras_zeiss_lightsheet_z1

2026-02-17T17:46:36+02:00

Microscope overview The Zeiss Lightsheet Z.1 is a light sheet fluorescence microscope able to image optical sections of large samples at subcellular resolution and very fast rates, with almost no phototoxicity or bleaching. It splits fluorescence excitation and detection into two separate light paths, with the axis of illumination being perpendicular to the detection axis.

oeiras_zeiss_lumar_v12

2026-02-17T18:02:37+02:00

Microscope overview The Stereo Lumar V.12 is a motorized stereoscope with fluorescence, capable of imaging in colour and grayscale (camera dependent), with a zoom between 9.6x and 120x. System components Filtersets Position Filterset Excitation

opt

2026-02-17T17:19:01+02:00

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optical_sec_course_2009

2020-08-03T17:48:45+02:00

BioImaging Workshop, 20 - 22 January 2009 Introduction Carl Zeiss MicroImaging and Instituto de Medicina Molecular are happy to invite you to the Advanced Optical Sectioning Microscopy Course, hosting the European premier of Zeiss On Your Campus Tour 2009

optical_sec_course_2009_rate

2020-08-03T17:52:49+02:00

BioImaging Workshop, 20 - 22 January 2009 How good was it? Student's feedback: 1 (bad); 2 (mediocre); 3 (sufficient); 4 (good); 5 (excellent) Everybody ---------- Back to the Bioimaging Courses

planning

2025-01-14T17:15:37+02:00

Spectra Viewers Objectives Specifications ---------- Back to the Bioimaging Wiki

prairie

2026-02-17T18:18:39+02:00

Microscope overview A multi-photon, or 2-photon, microscope uses modules similar to those of a laser scanning confocal microscope: A laser source for sample excitation, a scanhead with galvo mirrors to scan the excitation beam, and photo-multiplier tubes to detect fluorescence signals. 2-photon imaging has advantages over confocal: the use of a near-infrared laser allows deeper penetration into tissues, the radiation is less energetic and potentially less phototoxic, and because the excitation…

prototyping

2025-01-14T18:07:42+02:00

Conceptualisation and multi-scale fabrication of new devices and tools for applications in life sciences, so researchers can answer complex biological questions and accelerate their scientific findings – with a special focus on 3D rapid prototyping, microfluidics and micropatterning technologies and their integration with Bioimaging.

quality

2024-10-12T21:24:52+02:00

[Image by Maria Henriques, João Ferreira and José Rino] PSFs We image sub-resolution fluorescent beads (0.17 μm diameter) and use PSFj to analyze the acquired Point Spread Functions (PSFs) and determine the resolution in x, y and z for each objective. Illumination We measure the intensity of laser lines, LEDs and fluorescent lamps for each system.

quality_7live_2013

2020-08-01T03:22:57+02:00

Zeiss LSM 7 Live Quality Control 2013 PSFs Illumination ---------- Back to Zeiss LSM 7 Live Quality Control

quality_7live_2014

2020-08-01T03:06:04+02:00

Zeiss LSM 7 Live Quality Control 2014 PSFs Illumination ---------- Back to Zeiss LSM 7 Live Quality Control

quality_7live_2015

2016-03-10T00:13:07+02:00

Zeiss LSM 7 Live Quality Control 2015 PSFs Illumination Last update: 10/12/2015 ---------- Back to Zeiss LSM 7 Live Quality Control

quality_axiovert_2013

2020-08-01T03:22:11+02:00

Zeiss Axiovert 200M Quality Control 2013 PSFs Illumination ---------- Back to Quality Control

quality_axiovert_2014

2020-08-01T03:05:03+02:00

Zeiss Axiovert 200M Quality Control 2014 PSFs Illumination ---------- Back to Quality Control

quality_axiovert_2015

2020-08-01T02:42:55+02:00

Zeiss Axiovert 200M Quality Control 2015 PSFs Illumination ---------- Back to Quality Control

quality_axiovert_2016

2020-01-01T20:43:03+02:00

Zeiss Axiovert 200M Quality Control 2016 PSFs Illumination ---------- Back to Quality Control

quality_axiovert_2017

2020-08-01T01:33:45+02:00

Zeiss Axiovert 200M Quality Control 2017 PSFs Illumination ---------- Back to Zeiss Axiovert 200M Quality Control

quality_axiovert_2018

2020-08-01T00:50:41+02:00

Zeiss Axiovert 200M Quality Control 2018 PSFs Illumination ---------- Back to Zeiss Axiovert 200M Quality Control

quality_axiovert_2019

2020-08-13T17:20:05+02:00

Zeiss Axiovert 200M Quality Control 2019 PSFs Illumination ---------- Back to Zeiss Axiovert 200M Quality Control

quality_axiovert_2020

2021-04-05T23:35:14+02:00

Zeiss Axiovert 200M Quality Control 2020 PSFs Illumination ---------- Back to Zeiss Axiovert 200M Quality Control

quality_cell_observer_sd_2017

2020-08-01T01:31:53+02:00

Zeiss Cell Observer SD Quality Control - 2017 PSFs Illumination ---------- Back to Zeiss Cell Observer SD Quality Control

quality_cell_observer_sd_2018

2020-08-01T00:47:42+02:00

Zeiss Cell Observer SD Quality Control - 2018 PSFs Illumination ---------- Back to Zeiss Cell Observer SD Quality Control

quality_cell_observer_sd_2019

2021-11-30T13:51:56+02:00

Zeiss Cell Observer SD Quality Control - 2019 PSFs Illumination ---------- Back to Zeiss Cell Observer SD Quality Control

quality_cell_observer_sd_2020

2021-11-30T13:51:15+02:00

Zeiss Cell Observer SD Quality Control - 2020 PSFs Illumination ---------- Back to Zeiss Cell Observer SD Quality Control

quality_leica_sp8_mp_2018

2020-08-01T00:49:34+02:00

Leica SP8 MP Quality Control - 2018 PSFs Illumination ---------- Back to Leica SP8 MP Quality Control

quality_leica_sp8_mp_2019

2020-08-13T17:16:41+02:00

Leica SP8 MP Quality Control - 2019 PSFs Illumination ---------- Back to Leica SP8 MP Quality Control

quality_leica_sp8_mp_2020

2021-04-05T23:29:20+02:00

Leica SP8 MP Quality Control - 2020 PSFs Illumination ---------- Back to Leica SP8 MP Quality Control

quality_lsm710_2013

2020-08-01T03:21:13+02:00

Zeiss LSM 710 Quality Control 2013 PSFs Illumination ---------- Back to Quality Control

quality_lsm710_2014

2020-08-01T03:04:08+02:00

Zeiss LSM 710 Quality Control 2014 PSFs Illumination ---------- Back to Quality Control

quality_lsm710_2015

2020-08-01T02:41:49+02:00

Zeiss LSM 710 Quality Control 2015 PSFs Illumination ---------- Back to Quality Control

quality_lsm710_2016

2020-01-01T18:21:56+02:00

Zeiss LSM 710 Quality Control 2016 PSFs Illumination ---------- Back to Quality Control

quality_lsm710_2017

2020-08-01T01:32:50+02:00

Zeiss LSM 710 Quality Control 2017 PSFs Illumination ---------- Back to Zeiss LSM 710 Quality Control

quality_lsm710_2018

2020-08-01T00:48:25+02:00

Zeiss LSM 710 Quality Control 2018 PSFs Illumination ---------- Back to Zeiss LSM 710 Quality Control

quality_lsm710_2019

2020-08-13T17:14:53+02:00

Zeiss LSM 710 Quality Control 2019 PSFs Illumination ---------- Back to Zeiss LSM 710 Quality Control

quality_lsm710_2020

2021-04-05T23:27:02+02:00

Zeiss LSM 710 Quality Control 2020 PSFs Illumination ---------- Back to Zeiss LSM 710 Quality Control

quality_lumina_2020

2021-04-06T00:08:00+02:00

IVIS Lumina Quality Control 2020 Detection ---------- Back to IVIS Lumina Quality Control

quality_marianas_2013

2020-08-01T03:20:16+02:00

3i Marianas SDC Quality Control - 2013 PSFs Illumination ---------- Back to 3i Marianas SDC Quality Control

quality_marianas_2014

2020-08-01T03:01:17+02:00

3i Marianas SDC Quality Control - 2014 PSFs Illumination ---------- Back to 3i Marianas SDC Quality Control

quality_marianas_2015

2020-08-01T02:40:06+02:00

3i Marianas SDC Quality Control - 2015 PSFs Illumination ---------- Back to 3i Marianas SDC Quality Control

quality_marianas_2016

2019-12-31T18:24:17+02:00

3i Marianas SDC Quality Control - 2016 PSFs Illumination ---------- Back to 3i Marianas SDC Quality Control

quality_marianas_2017

2020-08-01T01:31:31+02:00

3i Marianas SDC Quality Control - 2017 PSFs Illumination ---------- Back to 3i Marianas SDC Quality Control

quality_marianas_2018

2020-08-01T00:47:18+02:00

3i Marianas SDC Quality Control - 2018 PSFs Illumination ---------- Back to 3i Marianas SDC Quality Control

quality_marianas_2019

2020-08-13T17:06:03+02:00

3i Marianas SDC Quality Control - 2019 PSFs Illumination ---------- Back to 3i Marianas SDC Quality Control

quality_marianas_2020

2021-11-24T17:32:34+02:00

3i Marianas SDC Quality Control - 2020 PSFs Illumination ---------- Back to 3i Marianas SDC Quality Control

quality_meta_2013

2020-08-01T03:23:55+02:00

Zeiss LSM 510 META Quality Control 2013 PSFs Illumination ---------- Back to Zeiss LSM 510 META Quality Control

quality_meta_2014

2020-08-01T03:07:01+02:00

Zeiss LSM 510 META Quality Control 2014 PSFs Illumination ---------- Back to Zeiss LSM 510 META Quality Control

quality_meta_2015

2016-06-27T17:00:09+02:00

Zeiss LSM 510 META Quality Control 2015 PSFs Illumination Last update: 10/12/2015 ---------- Back to Zeiss LSM 510 META Quality Control

quality_zeiss_cell_observer_2016

2020-01-01T20:09:36+02:00

Zeiss Cell Observer Quality Control 2016 PSFs Illumination ---------- Back to Quality Control

quality_zeiss_cell_observer_2017

2020-08-01T01:33:20+02:00

Zeiss Cell Observer Quality Control 2017 PSFs Illumination ---------- Back to Zeiss Cell Observer Quality Control

quality_zeiss_cell_observer_2018

2020-08-13T17:17:58+02:00

Zeiss Cell Observer Quality Control 2018 PSFs Illumination ---------- Back to Zeiss Cell Observer Quality Control

quality_zeiss_cell_observer_2019

2020-08-13T17:18:12+02:00

Zeiss Cell Observer Quality Control 2019 PSFs Illumination ---------- Back to Zeiss Cell Observer Quality Control

quality_zeiss_cell_observer_2020

2021-04-05T23:31:41+02:00

Zeiss Cell Observer Quality Control 2020 PSFs Illumination ---------- Back to Zeiss Cell Observer Quality Control

quality_zeiss_lsm_880_2016

2020-08-01T01:51:15+02:00

Zeiss LSM 880 Quality Control 2016 PSFs Illumination ---------- Back to Zeiss LSM 880 Quality Control

quality_zeiss_lsm_880_2017

2020-08-01T01:32:24+02:00

Zeiss LSM 880 Quality Control 2017 PSFs Illumination ---------- Back to Zeiss LSM 880 Quality Control

quality_zeiss_lsm_880_2018

2020-08-01T00:48:04+02:00

Zeiss LSM 880 Quality Control 2018 PSFs PSFs Illumination ---------- Back to Zeiss LSM 880 Quality Control

quality_zeiss_lsm_880_2019

2020-08-13T17:12:29+02:00

Zeiss LSM 880 Quality Control 2019 PSFs Illumination ---------- Back to Zeiss LSM 880 Quality Control

quality_zeiss_lsm_880_2020

2021-04-05T23:24:23+02:00

Zeiss LSM 880 Quality Control 2020 PSFs Illumination ---------- Back to Zeiss LSM 880 Quality Control

resources

2026-02-17T18:20:40+02:00

Widefield Systems agilent_cytation_5 deltavision nikon_eclipse_ti nikon_htm-hcs zeiss_apotome zeiss_axioscan_7 zeiss_axiovert_200m zeiss_cell_observer Spinning Disk Confocals 3i_marianas_sdc oeiras_3i_marianas_sdc oeiras_3i_marianas_sdc_bsl2 dragonfly leica_sdc zeiss_cell_observer_sd Point Scanning Confocals zeiss_celldiscoverer_7 zeiss_lsm_880 zeiss_lsm_900 zeiss_lsm_980 zeiss_lsm_980_oeiras zeiss_lsm_980-32 Light Sheet Microscopes blaze opt zeiss_lightsheet_z1 oeiras_z…

retubi

2020-08-03T13:57:32+02:00

ReTuBi Workshop - Hands-on Practical Course 2016 Description At the frontier between biophysics, molecular and cell biology, this workshop intends to cover recent advances in fluorescence microscopy enabling measurement and computational analysis of cellular activities. The main purpose of the workshop is to provide training in fluorescent microscopy, image analysis and data processing in order to potentiate individual research projects. The practical component of the Workshop will consist of …

retubi2

2020-08-03T13:57:47+02:00

ReTuBi Workshop 2nd edition - 2018 Description Quantitative fluorescence microscopy has become one of the most important technical advances for the study of cancer progression. The aim of this workshop is to cover the most recent advances in fluorescence microscopy, which enable the observation of cellular activity with unprecedented resolution. The workshop aims at potentiating the participants’ skills in microscopy, help them choosing the most suitable microscope for their particular researc…

retubi2_rate

2020-01-02T00:56:58+02:00

How good was it? Everybody ---------- Back to the Bioimaging Courses

retubi_rate

2020-01-02T01:01:13+02:00

How good was it? Participants ---------- Back to the Bioimaging Courses

rino_cv

2025-07-22T22:57:19+02:00

Head of Facility José Rino, PhD Publishing Name: José Rino Ciência ID: A117-FBDB-A27B Nationality: Portuguese E-mail address: jose.rino@gimm.pt ---------- Research History José Rino graduated in Technological Physics Engineering from Instituto Superior Técnico, Technical Univers…

rodrigues_cv

2025-03-21T16:37:40+02:00

Patrícia Rodrigues, MSc Publishing Name: Patrícia Porfírio-Rodrigues Ciência-ID: A513-8207-4997 Orcid-ID: 0009-0000-3062-3207 Nationality: Portuguese E-mail address: patricia.rodrigues@gimm.pt ---------- Research History Patrícia Rodrigues graduated in Biochemistry at Faculdade de Ciências da Universidade de Lisboa (FCUL) in 2018.

santos_cv

2025-01-14T13:39:17+02:00

Aida Santos, MSc Publishing Name: Aida P Lima Ciência-ID: 1B11-6E47-89AF Nationality: Portuguese E-mail address: aida.santos@gimm.pt ---------- Research History Aida Santos has a BSc in Biology from Instituto Superior de Agronomia, Universidade de lisboa and a MSc in Genetic Molecular Biology and Biomedicine from Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa.

software_cclab

2025-01-14T16:43:24+02:00

Cell Counter L*a*b* Count cells with specific nuclear and cytoplasmic staining in color images (immunohistochemical staining) ---------- Back to Software

software_cclab2

2025-01-14T16:44:23+02:00

Cell Counter L*a*b* 2 Count 2 different cell populations with blue and brown staining in color images (immunohistochemical staining) ---------- Back to Software

software_mcc4tiff

2025-01-14T16:47:01+02:00

Multichannel Cell Counter 4TIFF Count cells with labeled nuclei and specific fluorescent labeling combinations in the cytoplasm - for use with 4 TIF files ending in _c01.tif, _c02.tif, _c03.tif and _c04.tif ---------- Back to Software

software_mccczi

2025-01-14T16:45:08+02:00

Multichannel Cell Counter CZI Count cells with labeled nuclei and specific fluorescent labeling combinations in the cytoplasm - for use with Zeiss CZI files ---------- Back to Software

software_mcclsm

2025-01-14T16:45:46+02:00

Multichannel Cell Counter LSM Count cells with labeled nuclei and specific fluorescent labeling combinations in the cytoplasm - for use with Zeiss LSM files ---------- Back to Software

software_mccrgb

2025-01-14T16:46:30+02:00

Multichannel Cell Counter RGB Count cells with labeled nuclei and specific fluorescent labeling combinations in the cytoplasm - for use with RGB TIF files ---------- Back to Software

software_staqtool

2022-08-30T16:13:54+02:00

STaQTool - Spot Tracking and Quantification Tool STaQTool was developed for automated tracking of a transcription site in the cell nucleus, measuring its fluorescence intensity by Gaussian fitting and analyzing patterns of changes in fluorescence intensity over time. STaQTool runs in any PC with MATLAB Compiler Runtime. The software is a set of open-source MATLAB functions integrated into a single

start

2025-12-10T20:16:33+02:00

---------- ---------- ----------

statistics_lisbon

2025-07-27T20:45:24+02:00

---------- Back to Statistics

statistics_oeiras

2025-07-27T20:48:12+02:00

---------- Back to Statistics

super-resolution_workshop

2022-05-31T17:03:46+02:00

* Date: September 4 - 5, 2019 * Location: Instituto de Medicina Molecular João Lobo Antunes * Deadline for registration: August 24, 2019 About Super-resolution microscopy represents a group of recent techniques that can break the diffraction-limited resolution barrier (less than 200 nm). This workshop will introduce the principles and present the most recent advances of super-resolution microscopy, focusing on structured illumination microscopy (SIM). The workshop is designed for cel…

super-resolution_workshop_rate

2020-01-29T17:37:33+02:00

How good was it? ---------- Back to the Bioimaging Courses

systems_usage_global_years

2025-01-14T00:55:47+02:00

iMM Bioimaging Usage over the years (click to enlarge) ---------- Back to Lisbon Statistics

systems_usage_years

2022-10-15T14:04:07+02:00

Monthly Usage Hours Spinning Disk Confocal Microscopes Point Scanning Confocal Microscopes Multi-photon Microscopes Light Sheet Fluorescence Microscopes Widefield Fluorescence Microscopes Brightfield Microscopes Stereo Microscopes

tanqueiro_cv

2025-01-06T17:06:34+02:00

Manuel Tanqueiro, MSc Publishing Name: Manuel Tanqueiro Ciência-ID: BD12-D066-72EA Nationality: Portuguese E-mail address: manuel.tanqueiro@gimm.pt ---------- Research History Manuel Tanqueiro graduated in Biology @ Faculdade de Ciências da Universidade de Lisboa (FCUL), with a minor in Evolutionary and Developmental Biology in 2019. He then enrolled the Evolutionary and Developmental Biology mas…

team

2023-04-16T23:24:09+02:00

Meet the Bioimaging Unit Current Team Past Team Members ---------- Back to the Bioimaging Wiki

temudo_cv

2025-01-03T01:44:16+02:00

António Temudo, MSc Publishing Name: António Temudo Ciência-ID: Nationality: Portuguese E-mail address: antonio.temudo@gimm.pt ---------- Research History António Temudo has a Licenciatura in Biology and a MSc in Evolutionary and Developmental Biology from Faculdade de Ciências, Universidade de Lisboa.

top5

2022-03-15T11:56:54+02:00

BioImaging Resources - TOP 5 Users And the winners are... 2021 Pedro Campinho (CFRANCO) 751.5h Mariana De Niz (LFIGUEIREDO) 657h Diogo Coutinho (SDIAS) 616.5h 4 Katharina Hennig (EGOMES) 613h 5 Andreia Pena (CFRANCO)

top5_past

2017-03-26T19:55:05+02:00

BioImaging Resources - TOP 5 Users in the Past ALL SYSTEMS 3i Marianas SDC Zeiss LSM 7 Live Zeiss LSM 710 Zeiss LSM 510 META Zeiss Axiovert 200M Leica DM5000B Leica DMR Leica DM2500 IVIS Lumina

tutorials

2025-01-14T17:17:17+02:00

Educational Websites Online Books Online Courses and Videos Microcourses - practical instructional videos on various microscopy topics from Harvard Medical School CBMF Community ---------- Back to the Bioimaging Wiki

usage_policy

2025-07-22T22:34:28+02:00

Failure to adhere to these policies will result in a warning. Repeated violations may lead to loss of access. Scheduling and Access * • Before using any resource, new users must request access to the facility staff. * • Users must have a

usage_reports

2026-02-05T18:26:29+02:00

---------- Back to the Bioimaging Wiki

users_feedback

2025-01-14T10:40:02+02:00

Users Evaluation Survey The Satisfaction Survey aims at evaluating the services provided to researchers. Here are the results for the Bioimaging Unit. Thank you for your feedback! 2021 2022 2023

users_feedback_2013-2015

2022-01-15T13:17:24+02:00

Users Evaluation Survey 2013 - 2015 2013 2014 2015 N = 72 N = 73 N = 91 ---------- Back to Users Feedback

users_feedback_2016-2018

2022-01-15T13:17:05+02:00

Users Evaluation Survey 2016 - 2018 2016 2017 2018 N = 78 N = 76 N = 109 ---------- Back to Users Feedback

users_feedback_2019-2021

2023-01-04T17:56:58+02:00

Users Evaluation Survey 2019-2021 2019 2020 2021 N = 132 N = 115 N = 100 ---------- Back to Users Feedback

users_feedback_gimm

2025-10-25T17:00:51+02:00

Platform Satisfaction Survey The Satisfaction Survey aims at evaluating the services provided to researchers. Here are the results for the Bioimaging Platform. Thank you for your feedback! 2025 N = 88 ---------- Back to Statistics

users_feedback_past

2020-12-29T13:53:17+02:00

Users Evaluation Survey 2013 2014 2015 2016 N = 72 N = 73 N = 91 N = 78 ---------- Back to Users Feedback

users_feedback_past_2

2020-01-20T23:30:48+02:00

Users Evaluation Survey 2016 2017 2018 2019 N = 78 N = 76 N = 109 N = 132 ---------- Back to Users Feedback

workshop_hms_2010

2020-08-03T17:35:37+02:00

Quantitative Light Microscopy in Living Cells Description This five-day intensive workshop aims to provide a practical guide for quantitative live cell imaging in research, by bringing together cell biologists and microscopy experts in a relaxed environment to review the most relevant issues, explore advanced techniques and discuss the opportunities offered by the quantitative observation of cells in real-time while successfully keeping them alive. This advanced imaging workshop is aimed a…

z2_workstation

2025-01-10T17:14:45+02:00

System overview Z2 is a 64-bit PC running Windows 10, dedicated to light sheet imaging analysis, equipped with: * 2x Intel® Xeon Processor E5.2620 @ 2.4GHz * 256 GB DDR4 RAM memory * 2x 256 GB SSD + 36 TB disk space * 8 GB NVIDIA Quadro P4000 (graphics card)

z3_workstation

2026-04-17T09:41:08+02:00

System overview Z3 is a 64-bit PC running Windows 10, equipped with: * 2x Intel® Xeon Processor E5.2620 @ 2.4GHz * 128 GB DDR4 RAM memory * 2x 256 GB SSD + 36 TB disk space * 8 GB NVIDIA Quadro P4000 (graphics card) * 28“ TFT monitor

zeiss_510_meta_quality

2016-04-17T01:53:25+02:00

Zeiss LSM 510 META Quality Control 2015 Click here to get info on the Zeiss LSM 510 META PSFs Illumination Last update: 10/12/2015 ---------- -> Quality Control during 2014 -> Quality Control during 2013 ---------- Back to Quality Control

zeiss_apotome

2026-02-13T21:49:13+02:00

Microscope overview The ZEISS Axio Imager with Apotome 2 is a fully automated upright microscope capable of magnifications from 2.5x to 160x and equipped with two cameras: On the left port a 5MPx CMOS color camera (Axiocam 105 RGB, for brightfield/DIC) and on the top port a high sensitivity 4 Mpx monochrome sCMOS camera (

zeiss_axioscan_7

2026-02-13T21:50:45+02:00

Microscope overview The Zeiss Axioscan 7 is a high-performance automated slide scanner for brightfield and fluorescence imaging. It can scan up to 100 slides in a single run. It is equipped with a fully motorized condenser, LED illumination and sensitive color and monochromatic cameras for high-speed digitization of high quality images. Using the ZEN software you can assign scan profiles for acquisition runs to be set up quickly whether you have many similar slides or mixed applications to proc…

zeiss_axiovert200m_quality

2022-02-12T17:24:09+02:00

Zeiss Axiovert 200M Quality Control zeiss_axiovert_200m Click here to get info on the Zeiss Axiovert 200M PSFs Illumination ---------- Back to Quality Control

zeiss_axiovert_200m

2026-02-13T22:48:19+02:00

Microscope overview The Zeiss Axiovert 200M is a fully motorized inverted widefield fluorescence microscope ideal for live-cell imaging applications. Using the Metamorph software you can control the motorized filters, shutters and stage to simultaneously set up multi-color time-lapse imaging experiments in multiple stage positions. Its sensitive cooled CCD camera (

zeiss_axiovert_200m_repair

2016-04-09T04:08:40+02:00

Zeiss Axiovert 200M Repair History * 05/06/15 - The camera cooling system has been repaired * 02/06/15 - The camera cooling system is not working 2014 * 30/03/14 - A new 27“ monitor was installed, replacing the two 19” monitors that were being used

zeiss_axiovert_200m_usage

2020-03-03T21:08:33+02:00

Zeiss Axiovert 200M Usage Statistics zeiss_axiovert_200m Click here to get info on the Zeiss Axiovert 200M ---------- Back to Statistics

zeiss_axiozoom_v16

2026-02-17T18:09:37+02:00

Microscope overview The Zeiss Axio Zoom.V16 is a fluorescence stereo microscope suitable for imaging large samples such as complete organisms. The system is equipped with fluorescence filters for GFP and RFP and a monochromatic camera (AxioCam MRm). With the Zeiss Axio Zoom.V16 you can perform multi-channel, Z-stack and time-lapse image acquisition of your samples, using a 1x objective with a high numerical aperture of NA 0.25.

zeiss_axiozoom_v16_quality

2022-02-12T17:43:43+02:00

Zeiss AxioZoom V16 Quality Control zeiss_axiozoom_v16 Click here to get info on the Zeiss AxioZoom V16 Illumination ---------- Back to Quality Control

zeiss_axiozoom_v16_usage

2020-03-03T21:17:54+02:00

Zeiss AxioZoom V16 Usage Statistics zeiss_axiozoom_v16 Click here to get info on the Zeiss AxioZoom V16 ---------- Back to Statistics

zeiss_campus_2010

2020-08-03T17:05:35+02:00

BioImaging Workshop, 13 - 15 July 2010 Introduction Carl Zeiss MicroImaging and Instituto de Medicina Molecular are happy to invite you to the Zeiss On Your Campus - 2010 this year focused on live cell imaging and featuring the latest ZEISS microscopes and application solutions. As a microscopy user, you will have an excellent oportunity to learn about advanced techniques as well as use high-end systems. Get three days of inspiration from top imaging experts and colleagues in a relaxed atmos…

zeiss_campus_2010_rate

2020-08-03T17:12:52+02:00

BioImaging Workshop, 13 - 15 July 2009 How good was it? Student's feedback: 1 (bad); 2 (mediocre); 3 (sufficient); 4 (good); 5 (excellent) Everybody ---------- Back to the Bioimaging Courses

zeiss_cd7_usage

2024-07-17T01:45:29+02:00

Zeiss Celldiscoverer 7 with LSM 900 Usage Statistics zeiss_cell_observer Click here to get info on the Zeiss Celldiscoverer 7 with LSM 900 ---------- Back to Statistics

zeiss_cell_observer

2026-02-13T22:56:36+02:00

Microscope overview The Zeiss Cell Observer is a fully motorized inverted widefield fluorescence microscope ideal for live-cell imaging with fast acquisition speeds. It is equipped with a large cage incubator and a stage top incubator for temperature control and CO2 supply and with Definite Focus for hardware focus control during long time-lapse acquisitions. Using the ZEN software you can control the motorized filters, shutters and stage to simultaneously set up multi-color time-lapse imaging …

zeiss_cell_observer_quality

2022-02-12T17:19:56+02:00

Zeiss Cell Observer Quality Control zeiss_cell_observer Click here to get info on the Zeiss Cell Observer PSFs Illumination ---------- Back to Quality Control

zeiss_cell_observer_sd

2026-02-14T13:43:35+02:00

System overview The Zeiss Cell Observer SD spinning disk confocal microscope is a fast imaging system which provides a trade-off between confocality, resolution and speed. It is an inverted microscope ideal for live cell applications which require fast acquisition speeds rather than high resolution images. The scanning unit achieves confocality by directing light through a spinning disk with many small pinholes. Images are then acquired with a sensitive EMCCD which allows for very small exposu…

zeiss_cell_observer_sd_quality

2022-02-12T15:46:55+02:00

Zeiss Cell Observer SD Quality Control zeiss_cell_observer_sd Click here to get info on the Zeiss Cell Observer SD PSFs Illumination ---------- Back to Quality Control

zeiss_cell_observer_sd_usage

2020-03-03T20:58:22+02:00

Zeiss Cell Observer SD Usage Statistics zeiss_cell_observer_sd Click here to get info on the Zeiss Cell Observer SD ---------- Back to Statistics

zeiss_cell_observer_usage

2020-03-03T21:07:55+02:00

Zeiss Cell Observer Usage Statistics zeiss_cell_observer Click here to get info on the Zeiss Cell Observer ---------- Back to Statistics

zeiss_celldiscoverer_7

2026-04-17T01:14:07+02:00

Microscope overview The Zeiss Celldiscoverer 7 is a fully integrated and motorized high-end inverted widefield and point scanning confocal fluorescence imaging system ideal for automated live-cell imaging in multi-well plates and Petri dishes. It offers a fully integrated incubation system with temperature and CO2 control and

zeiss_lightsheet_z1

2026-02-17T17:28:08+02:00

zeiss_lightsheet_z1_usage

2020-03-03T21:02:20+02:00

Zeiss Lightsheet Z1 Usage Statistics zeiss_lightsheet_z1 Click here to get info on the Zeiss Lightsheet Z1 ---------- Back to Statistics

zeiss_lsm_7_live

2021-01-17T04:48:22+02:00

Microscope overview The Zeiss LSM 7 Live confocal line-scanning microscope is a fast imaging system able to analyze high-speed processes with a time resolution of just a few microseconds. It is an upright microscope equipped with water dipping objectives specially suited for live samples in aqueous media. The scanning unit uses a laser beam with a rectangular cross-section to illuminate a whole line in the sample, instead of a single point. This makes image acquisition by its array CCD detecto…

zeiss_lsm_7_live_quality

2020-08-01T02:45:49+02:00

Zeiss LSM 7 Live Quality Control 2015 Click here to get info on the Zeiss LSM 7 Live PSFs Illumination ---------- -> Quality Control during 2014 -> Quality Control during 2013 ---------- Back to Quality Control

zeiss_lsm_7_live_repair

2016-04-09T04:07:39+02:00

Zeiss LSM 7 Live Repair History * 02/05/12 - Zeiss LSM 5 Live has been upgraded to Zeiss LSM 7 Live! * 02/05/12 - Zeiss LSM 5 Live is now running ZEN 2009! * 05/04/12 - The real-time PC on the Zeiss LSM 5 Live is working again! * 13/02/12 - The real-time PC

zeiss_lsm_7_live_usage

2020-03-03T21:21:43+02:00

Zeiss LSM 7 Live Usage Statistics Click here to get info on the Zeiss LSM 7 Live Microscope is no longer in use ---------- Back to Statistics

zeiss_lsm_510

2023-08-06T13:34:09+02:00

Zeiss LSM 510 (no longer available) Location: no longer available Manufacturer: Zeiss Model: LSM 510 Year: 1998 SN: 450240 Microscope overview The LSM 510 system is a point scanning laser confocal microscope prepared for live imaging and photobleaching experiments. The system can be equipped with a small size incubator for temperature control.

zeiss_lsm_510_meta

2021-01-17T04:48:40+02:00

Microscope overview The Zeiss LSM 510 META is a confocal point-scanning microscope able to generate high-resolution three-dimensional images of thick specimens. It is an inverted microscope suitable for live cell imaging and photobleaching experiments, equipped with a large size incubator for temperature control and a small stage incubator for CO2 supply. Its META detector can be used for spectral detection and subsequent linear unmixing of overlapping fluorescent stainings, however at a much l…

zeiss_lsm_510_meta_quality

2020-08-01T02:47:41+02:00

Zeiss LSM 510 META Quality Control 2015 Click here to get info on the Zeiss LSM 510 META PSFs Illumination ---------- -> Quality Control during 2014 -> Quality Control during 2013 ---------- Back to Quality Control

zeiss_lsm_510_meta_repair

2016-04-17T02:03:28+02:00

Zeiss LSM 510 META Repair History * 17/06/15 - DPSS 561 nm laser is not working 2014 * 27/05/14 - A new Argon laser has been installed * 16/05/14 - The Argon laser is not working 2013 * 29/07/13 - A new Controller for the 405 nm laser has been installed

zeiss_lsm_510_meta_usage

2020-03-03T21:21:55+02:00

Zeiss LSM 510 META Usage Statistics Click here to get info on the Zeiss LSM 510 META Microscope is no longer in use ---------- Back to Statistics

zeiss_lsm_710

2024-01-18T01:18:12+02:00

Microscope overview The Zeiss LSM 710 is a confocal point-scanning microscope able to generate high-resolution three-dimensional images of thick specimens with high sensitivity and low photodamage. It is an inverted microscope especially suitable for live cell imaging and photobleaching experiments, equipped with a large cage incubator and a stage top incubator for temperature control and CO2 supply. The stage is motorized so imaging is possible in multiple stage positions or tiled acquisition…

zeiss_lsm_710_quality

2022-02-12T15:57:08+02:00

Zeiss LSM 710 Quality Control zeiss_lsm_710 Click here to get info on the Zeiss LSM 710 PSFs Illumination ---------- Back to Quality Control

zeiss_lsm_710_repair

2016-04-09T04:08:01+02:00

Zeiss LSM 710 Repair History * 14/11/12 - A new Ar laser was installed on the Zeiss LSM 710 * 23/01/12 - The X laser scanner on Zeiss LSM 710 is working again! 2011 * 22/12/11 - A new TFT monitor has been installed * 22/12/11 - The X laser scanner on Zeiss LSM 710 is malfunctioning

zeiss_lsm_710_usage

2020-03-03T20:58:59+02:00

Zeiss LSM 710 Usage Statistics zeiss_lsm_710 Click here to get info on the Zeiss LSM 710 ---------- Back to Statistics

zeiss_lsm_880

2026-02-17T16:43:18+02:00

Microscope overview The Zeiss LSM 880 with Airyscan is a point scanning confocal microscope able to generate high-resolution three-dimensional images of thick specimens with high sensitivity, high speed and low photodamage. It is an inverted microscope especially suitable for live cell imaging and photobleaching experiments, equipped with a large cage incubator and a stage top incubator for temperature control and CO2 supply and with Definite Focus for hardware focus control during long time-l…

zeiss_lsm_880_quality

2022-02-12T16:05:20+02:00

Zeiss LSM 880 Quality Control zeiss_lsm_880 Click here to get info on the Zeiss LSM 880 PSFs Illumination ---------- Back to Quality Control

zeiss_lsm_880_usage

2020-03-03T20:58:41+02:00

Zeiss LSM 880 Usage Statistics zeiss_lsm_880 Click here to get info on the Zeiss LSM 880 ---------- Back to Statistics

zeiss_lsm_900

2026-02-17T16:47:54+02:00

Microscope overview The Zeiss LSM 900 with Airyscan 2 is a point scanning confocal microscope able to generate high-resolution three-dimensional images of thick specimens with high sensitivity, high speed and low photodamage. It is an inverted microscope especially suitable for live cell imaging and photobleaching experiments, equipped with a dark large cage incubator and a stage top incubator for temperature control and CO2 supply and with Definite Focus.3 for hardware focus control during lo…

zeiss_lsm_980-32

2026-04-16T13:36:18+02:00

Microscope overview The Zeiss LSM 980-32 with Airyscan 2 is a point scanning confocal microscope able to generate high-resolution three-dimensional images of thick specimens with high sensitivity, high speed and low photodamage. It is an inverted microscope especially suitable for live cell imaging and photobleaching experiments, equipped with a dark large cage incubator and a stage top incubator for temperature control and CO2 supply, with Definite Focus.3 for hardware focus control during lo…

zeiss_lsm_980

2026-04-16T13:50:36+02:00

Microscope overview The Zeiss LSM 980 with Airyscan 2 is a point scanning confocal microscope able to generate high-resolution three-dimensional images of thick specimens with high sensitivity, high speed and low photodamage. It is an inverted microscope especially suitable for live cell imaging and photobleaching experiments, equipped with a dark large cage incubator and a stage top incubator for temperature control and CO2 supply and with Definite Focus.3 for hardware focus control during lo…

zeiss_lsm_980_oeiras

2026-02-17T16:55:57+02:00

Microscope overview The Zeiss LSM 980 with Airyscan 2 is an inverted point scanning confocal microscope equipped with a laser scanning head with a total of 6 PMT/GaAsp + 1 Airyscan detectors. The Airyscan can achieve non-diffraction limited optical sectioning with resolutions down to 140nm laterally and ~ 400nm axially. It also allows acquisition in “Multiplexing” modes, which accelerate the acquisition speed (4/8x faster) or in sensitivity mode that enhances the signal-to-noise acquisition.…

zeiss_lsm_980_quality

2022-09-01T15:13:27+02:00

Zeiss LSM 980 Quality Control zeiss_lsm_980 Click here to get info on the Zeiss LSM 980 PSFs Illumination ---------- Back to Quality Control

zeiss_lsm_980_usage

2022-09-01T15:16:39+02:00

Zeiss LSM 980 Usage Statistics zeiss_lsm_980 Click here to get info on the Zeiss LSM 980 ---------- Back to Statistics

zeiss_lumar_v12

2021-04-09T16:45:19+02:00

Microscope overview The Zeiss Lumar V12 is a fluorescence stereo microscope suitable for high resolution 3D analysis of large specimens in fluorescent and transmitted light. The system is equipped with fluorescence filters for GFP, Cy3 and Cy5.5 and a sensitive cooled CCD camera (Hamamatsu ORCA-R2) that allows you to capture weak fluorescent signals and minimize photobleaching in light sensitive samples. With the Zeiss Lumar V12 you can perform multi-channel, Z-stack and time-lapse image acquis…

zeiss_lumar_v12_usage

2020-03-03T21:18:22+02:00

Zeiss Lumar V12 Usage Statistics zeiss_lumar_v12 Click here to get info on the Zeiss Lumar V12 ---------- Back to Statistics