GIMM Bioimaging Wiki

about

bioimaging — 2026-08-11T18:03:33+02:00

About the Bioimaging Platform The Bioimaging Platform offers state-of-the-art personalized support in all steps of research done with Light Microscopy at GIMM, housing 30 systems including point-scanning confocal, spinning disk confocal, multiphoton, light sheet, widefield, slide scanning, and advanced image analysis workstations.

maps_oeiras

bioimaging — 2026-08-11T14:18:39+02:00

Where are the microscopes? ---------- Back to the Equipment page

bartolomeu_dias_wing_2026.png - created

bioimaging — 2026-08-11T14:18:14+02:00

maps

bioimaging — 2026-08-11T14:10:20+02:00

Where are the microscopes? ---------- Back to the Equipment page

all_p2_map_2026.png - created

bioimaging — 2026-08-11T14:10:06+02:00

all_p0_map_2026.png - created

bioimaging — 2026-08-11T14:10:06+02:00

prairie

bioimaging — 2026-08-11T12:58:43+02:00

Microscope overview A multi-photon, or 2-photon, microscope uses modules similar to those of a laser scanning confocal microscope: A laser source for sample excitation, a scanhead with galvo mirrors to scan the excitation beam, and photo-multiplier tubes to detect fluorescence signals. 2-photon imaging has advantages over confocal: the use of a near-infrared laser allows deeper penetration into tissues, the radiation is less energetic and potentially less phototoxic, and because the excitation…

bartolomeu_dias_wing_2025_-_prairie.png

bioimaging — 2026-08-11T12:58:17+02:00

bartolomeu_dias_wing_2025_-_3i_marianas_sdc_sora_ablation_.png

bioimaging — 2026-08-11T12:49:55+02:00

bartolomeu_dias_wing_2025_-_dragonfly.png

bioimaging — 2026-08-11T12:49:54+02:00

bartolomeu_dias_wing_2025_-_3i_marianas_sdc_tirf_.png

bioimaging — 2026-08-11T12:49:54+02:00

oeiras_3i_marianas_sdc_bsl2

bioimaging — 2026-08-11T12:32:24+02:00

Microscope overview The 3i Marianas SDC II super-resolution spinning disk confocal microscope is a fast imaging system which provides a trade-off between confocality, resolution and speed, well suited for the observation of dynamics in living cells and small organisms. The Marianas SDC provides fast acquisition (up to ~100fps, with limited ROI, ~30FPS full frame), and with lower phototoxicity and bleaching. A spinning disk with multiple small pinholes is installed between the light source and …

oeiras_3i_marianas_sdc_sora_tirf_bsl2_name.png - created

bioimaging — 2026-08-11T12:32:10+02:00

oeiras_3i_marianas_sdc

bioimaging — 2026-08-11T12:26:13+02:00

Microscope overview The 3i Marianas SDC spinning disk confocal microscope is a fast imaging system which provides a combination of confocality, resolution and speed, well suited for the observation of dynamics in living cells and small organisms. The Marianas SDC provides fast acquisition (up to ~100fps, with limited ROI, ~30FPS full frame), and with lower phototoxicity and bleaching. A spinning disk with multiple small pinholes is installed between the light source and specimen to generate po…

oeiras_3i_marianas_sdc_sora_ablation_name.png - created

bioimaging — 2026-08-11T12:23:54+02:00

3i_marianas_sdc

bioimaging — 2026-08-11T12:21:28+02:00

System overview The 3i Marianas SDC spinning disk confocal microscope is a fast imaging system which provides a trade-off between confocality, resolution and speed. It is an inverted microscope ideal for live cell applications which require fast acquisition speeds rather than high resolution images. The scanning unit achieves confocality by directing light through a spinning disk with many small pinholes. Images are then acquired with a sensitive EMCCD which allows for very small exposure time…

oeiras_3i_marianas_sdc_tirf_name.png - created

bioimaging — 2026-08-11T12:16:23+02:00

resources - [Brightfield / Stereo Microscopes]

bioimaging — 2026-08-11T11:51:47+02:00

Point Scanning Confocals leica_stellaris_sted_flim zeiss_celldiscoverer_7 zeiss_lsm_880 zeiss_lsm_900 zeiss_lsm_980 zeiss_lsm_980_oeiras zeiss_lsm_980-32 Widefield Systems agilent_cytation_5 nikon_eclipse_ti nikon_htm-hcs zeiss_apotome zeiss_axioscan_7 zeiss_axiovert_200m zeiss_cell_observer Spinning Disk Confocals oeiras_3i_marianas_sdc oeiras_3i_marianas_sdc_bsl2 3i_marianas_sdc dragonfly leica_sdc zeiss_cell_observer_sd Light Sheet Microscopes blaze opt zeiss_lights…

oeiras_-_3i_marianas_sdc_sora_tirf_bsl2_with_name.png - created

bioimaging — 2026-08-11T11:47:32+02:00

oeiras_-_3i_marianas_sdc_sora_ablation_with_name.png - created

bioimaging — 2026-08-11T11:47:32+02:00