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zeiss_lumar_v12 [2020/01/01 23:21]
bioimaging [Objectives]
zeiss_lumar_v12 [2020/06/01 19:26] (current)
bioimaging
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 &nbsp <br> &nbsp <br>
 &nbsp <br> &nbsp <br>
-&rarr; <a href="​https://​imm.medicina.ulisboa.pt/​intranet/booking/58"><​img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=date.png"> ​ Zeiss Lumar V12 Booking</​a>​+&rarr; <a href="​https://​my.agendo.science/calendar/?​id=MzFjYWxlbmRhcnM="><​img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=date.png"> ​ Zeiss Lumar V12 Booking</​a>​
  <​br> ​  <​br> ​
 &rarr; <a href="/​facility/​bioimaging/​doku.php?​id=zeiss_lumar_v12_usage"><​img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=chart_line.png"> ​ Zeiss Lumar V12 Usage Statistics</​a>​ &rarr; <a href="/​facility/​bioimaging/​doku.php?​id=zeiss_lumar_v12_usage"><​img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=chart_line.png"> ​ Zeiss Lumar V12 Usage Statistics</​a>​
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 ===== Microscope overview ===== ===== Microscope overview =====
-The Zeiss Lumar V12 is a //​fluorescence stereo microscope//​ suitable for high resolution 3D analysis of large specimens in fluorescent and transmitted light. The system is equipped with fluorescence filters for GFP, Cy3 and Cy5.5 and a sensitive cooled CCD camera ([[http://​www.hamamatsu.com/​jp/​en/​community/​life_science_camera/​product/​search/​C10600-10B/​index.html|ORCA R2]]) that allows you to capture weak fluorescent signals and minimize photobleaching in light sensitive samples. With the Zeiss Lumar V12 you can perform multi-channel,​ Z-stack and time-lapse image acquisition of your samples, using two different objectives with different working distances and magnification values. If you need higher magnifications,​ check out the [[leica_dm5000b|Leica DM5000B]], the [[zeiss_axiovert_200m|Zeiss Axiovert 200M]] or the [[zeiss_cell_observer|Zeiss Cell Observer]] widefield microscopes.+The Zeiss Lumar V12 is a //​fluorescence stereo microscope//​ suitable for high resolution 3D analysis of large specimens in fluorescent and transmitted light. The system is equipped with fluorescence filters for GFP, Cy3 and Cy5.5 and a sensitive cooled CCD camera (Hamamatsu ​ORCA-R2) that allows you to capture weak fluorescent signals and minimize photobleaching in light sensitive samples. With the Zeiss Lumar V12 you can perform multi-channel,​ Z-stack and time-lapse image acquisition of your samples, using two different objectives with different working distances and magnification values. If you need higher magnifications,​ check out the [[leica_dm5000b|Leica DM5000B]], the [[zeiss_axiovert_200m|Zeiss Axiovert 200M]] or the [[zeiss_cell_observer|Zeiss Cell Observer]] widefield microscopes.
  
 {{:​warning.png?​25|}} Data files older than **3 months** will be automatically deleted on this system, please copy your data to the iMM server using the desktop link. {{:​warning.png?​25|}} Data files older than **3 months** will be automatically deleted on this system, please copy your data to the iMM server using the desktop link.
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 ==== Filtersets ==== ==== Filtersets ====
  
-^  Position ​ ^  Filterset ​ ^  Reference ​ ^  Excitation ​ ^  Emission ​ ^ +^  Position ​ ^  Filterset ​ ^  Reference ​ ^  Excitation ​ ​^ ​ Dichroic ​ ​^ ​ Emission ​ ^ 
-|  1  |  Empty  |  ​None  ​ ​None  ​ ​None  ​+|  1  |  Empty  ||||
-|  2  |  GFP  |  [[https://​www.micro-shop.zeiss.com/?​s=1249886915f0649&​l=en&​p=us&​f=f&​a=v&​b=f&​id=488013-0000-000&​o=|FS13]] ​ |  460-480 nm  |  505-530 nm  | +|  2  |  GFP  |  [[https://​www.micro-shop.zeiss.com/?​s=1249886915f0649&​l=en&​p=us&​f=f&​a=v&​b=f&​id=488013-0000-000&​o=|FS13]] ​ |  460-480 ​nm  |  495 nm  |  505-530 nm  | 
-|  3  |  Cy3  |  [[https://​www.micro-shop.zeiss.com/?​s=1249886915f0649&​l=en&​p=us&​f=f&​a=v&​b=f&​id=000000-1114-101&​o=|FS43]] ​ |  532-557 nm  |  570-640 nm  | +|  3  |  Cy3  |  [[https://​www.micro-shop.zeiss.com/?​s=1249886915f0649&​l=en&​p=us&​f=f&​a=v&​b=f&​id=000000-1114-101&​o=|FS43]] ​ |  532-557 ​nm  |  570 nm  |  570-640 nm  | 
-|  4  |  Cy5.5  |  [[https://​www.micro-shop.zeiss.com/?​s=1249886915f0649&​l=en&​p=us&​f=f&​a=v&​b=f&​id=000000-1031-354&​o=|FS32]] ​ |  652-677nm ​ |  700-750 nm  |+|  4  |  Cy5.5  |  [[https://​www.micro-shop.zeiss.com/?​s=1249886915f0649&​l=en&​p=us&​f=f&​a=v&​b=f&​id=000000-1031-354&​o=|FS32]] ​ |  652-677nm ​ ​| ​ 695 nm  ​| ​ 700-750 nm  |
  
 ==== Objectives ==== ==== Objectives ====
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 ^  Magnification ​  ​^ ​ Model  ^  WD (mm)  ^  About  ^ ^  Magnification ​  ​^ ​ Model  ^  WD (mm)  ^  About  ^
 |  1.5x  |  NeoLumar S  |  30  |  for visual magnification from 12x to 150x with eyepieces 10x  | |  1.5x  |  NeoLumar S  |  30  |  for visual magnification from 12x to 150x with eyepieces 10x  |
 +
 +==== Camera ====
 +^  Model  ^  Type  ^  Frame Size  ^  Pixel Size (µm)  ^
 +|  Hamamatsu ORCA-R2 |  CCD  |  1344 X 1024  |  6.45 x 6.45  |
  
  
zeiss_lumar_v12.1577917314.txt.gz · Last modified: 2020/01/01 23:21 by bioimaging