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zeiss_lumar_v12 [2016/04/09 03:56] bioimaging created |
zeiss_lumar_v12 [2021/04/09 16:45] (current) bioimaging |
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- | <img src="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_lumar_v12_name.jpg"> | + | <img src="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_lumar_v12_name_new.png"> |
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- | <a href="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_lumar_v12_wiki.jpg"><img src="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_lumar_v12_wiki.jpg" width=300></a> | + | <a href="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_lumar_v12_wiki_new.png"><img src="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_lumar_v12_wiki_new.png" width=300></a> |
<td style="border:0px solid white;"> <p style="line-height:1.8"> | <td style="border:0px solid white;"> <p style="line-height:1.8"> | ||
- | <b>Location</b>: <a href="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_lumar_v12_map.jpg">Room P3-A-44</a><br> | + | <b>Location</b>: iMM Fish Facility<br> |
<b>Manufacturer</b>: <a href="http://www.zeiss.de/micro" target="_blank">Carl Zeiss MicroImaging</a><br> | <b>Manufacturer</b>: <a href="http://www.zeiss.de/micro" target="_blank">Carl Zeiss MicroImaging</a><br> | ||
<b>Model</b>: SteREO Lumar V.12Axio Zoom.V16<br> | <b>Model</b>: SteREO Lumar V.12Axio Zoom.V16<br> | ||
+ | <b>Nickname</b>: "Lumar"<br> | ||
<b>Software</b>: AxioVision<br> | <b>Software</b>: AxioVision<br> | ||
<b>Year</b>: 2009 <br> | <b>Year</b>: 2009 <br> | ||
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- |   <br> | + | → <a href="https://my.agendo.science/calendar/?id=MzFjYWxlbmRhcnM="><img src="/facility/bioimaging/lib/exe/fetch.php?media=date.png"> Zeiss Lumar V12 Booking</a> |
- | → <a href="https://imm.medicina.ulisboa.pt/intranet/booking/month.php?user=ZEISSLUMA&rid=58"><img src="/facility/bioimaging/lib/exe/fetch.php?media=date.png"> Zeiss Lumar V12 Booking</a> | + | |
<br> | <br> | ||
→ <a href="/facility/bioimaging/doku.php?id=zeiss_lumar_v12_usage"><img src="/facility/bioimaging/lib/exe/fetch.php?media=chart_line.png"> Zeiss Lumar V12 Usage Statistics</a> | → <a href="/facility/bioimaging/doku.php?id=zeiss_lumar_v12_usage"><img src="/facility/bioimaging/lib/exe/fetch.php?media=chart_line.png"> Zeiss Lumar V12 Usage Statistics</a> | ||
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+ | ===== Microscope overview ===== | ||
+ | The Zeiss Lumar V12 is a //fluorescence stereo microscope// suitable for high resolution 3D analysis of large specimens in fluorescent and transmitted light. The system is equipped with fluorescence filters for GFP, Cy3 and Cy5.5 and a sensitive cooled CCD camera (Hamamatsu ORCA-R2) that allows you to capture weak fluorescent signals and minimize photobleaching in light sensitive samples. With the Zeiss Lumar V12 you can perform multi-channel, Z-stack and time-lapse image acquisition of your samples, using two different objectives with different working distances and magnification values. If you need higher magnifications, check out the [[leica_dm5000b|Leica DM5000B]], the [[zeiss_axiovert_200m|Zeiss Axiovert 200M]] or the [[zeiss_cell_observer|Zeiss Cell Observer]] widefield microscopes. | ||
+ | |||
+ | {{:warning.png?25|}} Data files older than **3 months** will be automatically deleted on this system, please copy your data to the iMM server using the desktop link. | ||
+ | |||
+ | ===== System components ===== | ||
+ | |||
+ | ==== Filtersets ==== | ||
+ | |||
+ | ^ Position ^ Filterset ^ Reference ^ Excitation ^ Dichroic ^ Emission ^ | ||
+ | | 1 | Empty ||||| | ||
+ | | 2 | GFP | [[https://www.micro-shop.zeiss.com/?s=1249886915f0649&l=en&p=us&f=f&a=v&b=f&id=488013-0000-000&o=|FS13]] | 460-480 nm | 495 nm | 505-530 nm | | ||
+ | | 3 | Cy3 | [[https://www.micro-shop.zeiss.com/?s=1249886915f0649&l=en&p=us&f=f&a=v&b=f&id=000000-1114-101&o=|FS43]] | 532-557 nm | 570 nm | 570-640 nm | | ||
+ | | 4 | Cy5.5 | [[https://www.micro-shop.zeiss.com/?s=1249886915f0649&l=en&p=us&f=f&a=v&b=f&id=000000-1031-354&o=|FS32]] | 652-677nm | 695 nm | 700-750 nm | | ||
+ | |||
+ | ==== Objectives ==== | ||
+ | ^ Magnification ^ Model ^ WD (mm) ^ About ^ | ||
+ | | 0.8x | NeoLumar S | 80 | for visual magnification from 6.4x to 80x with eyepieces 10x | | ||
+ | |||
+ | **Upon Request:** | ||
+ | ^ Magnification ^ Model ^ WD (mm) ^ About ^ | ||
+ | | 1.5x | NeoLumar S | 30 | for visual magnification from 12x to 150x with eyepieces 10x | | ||
+ | |||
+ | ==== Camera ==== | ||
+ | ^ Model ^ Type ^ Frame Size ^ Pixel Size (µm) ^ | ||
+ | | Hamamatsu ORCA-R2 | CCD | 1344 X 1024 | 6.45 x 6.45 | | ||
+ | |||
+ | |||
+ | ===== Software Modules ===== | ||
+ | |||
+ | * Multichannel Acquisition | ||
+ | * Z-stack Acquisition | ||
+ | * Extended Focus | ||
+ | |||
+ | ---- | ||
+ | [[resources|Back to the Equipment page]] |