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zeiss_lumar_v12 [2016/04/09 03:56]
bioimaging created
zeiss_lumar_v12 [2020/06/01 19:26] (current)
bioimaging
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 <​html>​ <​html>​
-<img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=zeiss_lumar_v12_name.jpg">+<img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=zeiss_lumar_v12_name_new.png">
 <table style="​border:​0px solid white;">​ <table style="​border:​0px solid white;">​
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-<a href="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=zeiss_lumar_v12_wiki.jpg"><​img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=zeiss_lumar_v12_wiki.jpg" width=300></​a>​+<a href="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=zeiss_lumar_v12_wiki_new.png"><​img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=zeiss_lumar_v12_wiki_new.png" width=300></​a>​
 <td style="​border:​0px solid white;">​ <p style="​line-height:​1.8">​ <td style="​border:​0px solid white;">​ <p style="​line-height:​1.8">​
-<​b>​Location</​b>:​ <a href="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=zeiss_lumar_v12_map.jpg">​Room ​P3-A-44</​a><​br> ​+<​b>​Location</​b>:​ <a href="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=zeiss_lumar_v12_map_new.png">​Room ​P0-B-32</​a><​br> ​
 <​b>​Manufacturer</​b>:​ <a href="​http://​www.zeiss.de/​micro"​ target="​_blank">​Carl Zeiss MicroImaging</​a><​br>​ <​b>​Manufacturer</​b>:​ <a href="​http://​www.zeiss.de/​micro"​ target="​_blank">​Carl Zeiss MicroImaging</​a><​br>​
 <​b>​Model</​b>:​ SteREO Lumar V.12Axio Zoom.V16<​br>​ <​b>​Model</​b>:​ SteREO Lumar V.12Axio Zoom.V16<​br>​
 +<​b>​Nickname</​b>:​ "​Lumar"<​br>​
 <​b>​Software</​b>:​ AxioVision<​br>​ <​b>​Software</​b>:​ AxioVision<​br>​
 <​b>​Year</​b>:​ 2009 <br> <​b>​Year</​b>:​ 2009 <br>
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 &nbsp <br> &nbsp <br>
 &nbsp <br> &nbsp <br>
-&nbsp <​br>​ +&rarr; <a href="​https://​my.agendo.science/calendar/?id=MzFjYWxlbmRhcnM="><​img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=date.png"> ​ Zeiss Lumar V12 Booking</​a>​
-&rarr; <a href="​https://​imm.medicina.ulisboa.pt/intranet/booking/​month.php?user=ZEISSLUMA&​rid=58"><​img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=date.png"> ​ Zeiss Lumar V12 Booking</​a>​+
  <​br> ​  <​br> ​
 &rarr; <a href="/​facility/​bioimaging/​doku.php?​id=zeiss_lumar_v12_usage"><​img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=chart_line.png"> ​ Zeiss Lumar V12 Usage Statistics</​a>​ &rarr; <a href="/​facility/​bioimaging/​doku.php?​id=zeiss_lumar_v12_usage"><​img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=chart_line.png"> ​ Zeiss Lumar V12 Usage Statistics</​a>​
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 </​html>​ </​html>​
  
 +===== Microscope overview =====
 +The Zeiss Lumar V12 is a //​fluorescence stereo microscope//​ suitable for high resolution 3D analysis of large specimens in fluorescent and transmitted light. The system is equipped with fluorescence filters for GFP, Cy3 and Cy5.5 and a sensitive cooled CCD camera (Hamamatsu ORCA-R2) that allows you to capture weak fluorescent signals and minimize photobleaching in light sensitive samples. With the Zeiss Lumar V12 you can perform multi-channel,​ Z-stack and time-lapse image acquisition of your samples, using two different objectives with different working distances and magnification values. If you need higher magnifications,​ check out the [[leica_dm5000b|Leica DM5000B]], the [[zeiss_axiovert_200m|Zeiss Axiovert 200M]] or the [[zeiss_cell_observer|Zeiss Cell Observer]] widefield microscopes.
 +
 +{{:​warning.png?​25|}} Data files older than **3 months** will be automatically deleted on this system, please copy your data to the iMM server using the desktop link.
 +
 +===== System components =====
 +
 +==== Filtersets ====
 +
 +^  Position ​ ^  Filterset ​ ^  Reference ​ ^  Excitation ​ ^  Dichroic ​ ^  Emission ​ ^
 +|  1  |  Empty  |||||
 +|  2  |  GFP  |  [[https://​www.micro-shop.zeiss.com/?​s=1249886915f0649&​l=en&​p=us&​f=f&​a=v&​b=f&​id=488013-0000-000&​o=|FS13]] ​ |  460-480 nm  |  495 nm  |  505-530 nm  |
 +|  3  |  Cy3  |  [[https://​www.micro-shop.zeiss.com/?​s=1249886915f0649&​l=en&​p=us&​f=f&​a=v&​b=f&​id=000000-1114-101&​o=|FS43]] ​ |  532-557 nm  |  570 nm  |  570-640 nm  |
 +|  4  |  Cy5.5  |  [[https://​www.micro-shop.zeiss.com/?​s=1249886915f0649&​l=en&​p=us&​f=f&​a=v&​b=f&​id=000000-1031-354&​o=|FS32]] ​ |  652-677nm ​ |  695 nm  |  700-750 nm  |
 +
 +==== Objectives ====
 +^  Magnification ​  ​^ ​ Model  ^  WD (mm)  ^  About  ^
 +|  0.8x  |  NeoLumar S  |  80  |  for visual magnification from 6.4x to 80x with eyepieces 10x  |
 +
 +**Upon Request:**
 +^  Magnification ​  ​^ ​ Model  ^  WD (mm)  ^  About  ^
 +|  1.5x  |  NeoLumar S  |  30  |  for visual magnification from 12x to 150x with eyepieces 10x  |
 +
 +==== Camera ====
 +^  Model  ^  Type  ^  Frame Size  ^  Pixel Size (µm)  ^
 +|  Hamamatsu ORCA-R2 |  CCD  |  1344 X 1024  |  6.45 x 6.45  |
 +
 +
 +===== Software Modules =====
 +
 +  * Multichannel Acquisition
 +  * Z-stack Acquisition
 +  * Extended Focus
 +
 +----
 +[[resources|Back to the Resources page]]
zeiss_lumar_v12.1460166962.txt.gz · Last modified: 2016/04/09 03:56 by bioimaging