zeiss_lsm_980
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zeiss_lsm_980 [2022/09/02 00:22] bioimaging [Objectives] |
zeiss_lsm_980 [2024/04/02 00:22] (current) bioimaging |
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| <b>Model</b>: <a href="https://www.zeiss.com/microscopy/en/products/light-microscopes/confocal-microscopes/lsm-980-with-airyscan-2.html">LSM 980 with Airyscan 2</a><br> | <b>Model</b>: <a href="https://www.zeiss.com/microscopy/en/products/light-microscopes/confocal-microscopes/lsm-980-with-airyscan-2.html">LSM 980 with Airyscan 2</a><br> | ||
| <b>Nickname</b>: "980"<br> | <b>Nickname</b>: "980"<br> | ||
| - | <b>Software</b>: ZEN 3.5 (blue edition)<br> | + | <b>Software</b>: ZEN 3.8 (blue edition)<br> |
| <b>Year</b>: 2021 <br> | <b>Year</b>: 2021 <br> | ||
| <b>SN</b>: 2841000239 <br> | <b>SN</b>: 2841000239 <br> | ||
|   <br> |   <br> | ||
| + | Data will be deleted after: <b>1 month</b> | ||
|   <br> |   <br> | ||
|   <br> |   <br> | ||
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| </table> | </table> | ||
| </html> | </html> | ||
| - | |||
| - | ** page under construction ** | ||
| ===== Microscope overview ===== | ===== Microscope overview ===== | ||
| - | {{ :confocal_pinhole.png?350}} The Zeiss LSM 980 with Airyscan 2 is a [[https://www.youtube.com/watch?v=rTkjQ6Q0p5I|point-scanning confocal microscope]] able to generate high-resolution three-dimensional images of thick specimens with high sensitivity, high speed and low photodamage. It is an inverted microscope especially suitable for live cell imaging and photobleaching experiments, equipped with a dark large cage incubator and a stage top incubator for temperature control and CO2 supply and with Definite Focus.2 for hardware focus control during long time-lapse acquisitions. The stage is motorized and furthermore equipped with a piezo for Z displacement so fast 4D imaging is possible in multiple stage positions, using the Tiles Advanced Setup of ZEN blue. Its [[http://www.zeiss.com/content/dam/Microscopy/Products/laser-scan/LSM%20880/zeiss-lsm880-airyscan-principle-672.jpg|Airyscan 2]] with 32 concentrically arranged GaAsP detection elements is able to collect all light from an Airy pattern simultaneously, allowing for increased resolution (120 nm laterally and 400 nm axially in SR mode, at 488 nm). It can also be used in [[https://www.youtube.com/watch?v=tUiEa7vcggQ|Multiplex Mode]], which enables parallel excitation and detection in 4Y or 8Y modes. The result is a speed improvement by a factor of 4x/8x, while maintaining the sensitivity of Airyscan. Its scanning unit further includes two PMT detectors as well as a GaAsP detector for increased sensitivity (45% QE compared to ~25% QE for conventional PMT). It is equipped with lasers from violet to far red (405, 488, 561 and 639 nm excitation wavelengths). With this system you can perform optical sectioning of fluorescent samples which are too thick for a widefield system such as the [[zeiss_cell_observer|Zeiss Cell Observer]]. Image resolution with Airyscan 2 is higher than the [[zeiss_lsm_710|Zeiss LSM 710]] and detection sensitivity is also higher than the spinning disks [[3i_marianas_sdc|3i Marianas SDC]] and [[zeiss_cell_observer_sd|Zeiss Cell Observer SD]]. If your personal computer cannot handle all the data you collected, check out the [[colossus|Colossus]]. | + | {{ :confocal_pinhole.png?340}} The Zeiss LSM 980 with Airyscan 2 is a [[https://www.youtube.com/watch?v=rTkjQ6Q0p5I|point-scanning confocal microscope]] able to generate high-resolution three-dimensional images of thick specimens with high sensitivity, high speed and low photodamage. It is an inverted microscope especially suitable for live cell imaging and photobleaching experiments, equipped with a dark large cage incubator and a stage top incubator for temperature control and CO2 supply and with Definite Focus.3 for hardware focus control during long time-lapse acquisitions. The stage is motorized and furthermore equipped with a piezo for Z displacement so fast 4D imaging is possible in multiple stage positions, using the Tiles Advanced Setup of ZEN blue. Its [[http://www.zeiss.com/content/dam/Microscopy/Products/laser-scan/LSM%20880/zeiss-lsm880-airyscan-principle-672.jpg|Airyscan 2]] with 32 concentrically arranged GaAsP detection elements is able to collect all light from an Airy pattern simultaneously, allowing for increased resolution (120 nm laterally and 350 nm axially in SR mode, at 488 nm). It can also be used in [[https://www.youtube.com/watch?v=tUiEa7vcggQ|Multiplex Mode]], which enables parallel excitation and detection in 4Y or 8Y modes. The result is a speed improvement by a factor of 4x/8x, while maintaining the sensitivity of Airyscan. Its scanning unit further includes two PMT detectors as well as a GaAsP detector for increased sensitivity (45% QE compared to ~25% QE for conventional PMT). It is equipped with lasers from violet to far red (405, 488, 561 and 639 nm excitation wavelengths). The [[https://www.zeiss.com/microscopy/en/about-us/newsroom/press-releases/2021/zeiss-lsm-plus-and-zeiss-airyscan-joint-deconvolution.html|LSM Plus deconvolution]] mode enhances signal-to-noise ratio by acting as a denoising step post image acquisition, allowing for high acquisition speed and low laser power. With [[https://www.zeiss.com/microscopy/en/about-us/newsroom/press-releases/2021/zeiss-lsm-plus-and-zeiss-airyscan-joint-deconvolution.html|Airyscan Joint Deconvolution (jDCV)]] the resolution can be improved to 90 nm (XY) and 270 nm (Z). |
| + | |||
| + | * **Microscope**: [[https://www.zeiss.com/microscopy/en/products/light-microscopes/widefield-microscopes/axio-observer-for-life-science-research.html|Zeiss Axio Observer]] | ||
| + | * **LED Illumination**: [[https://www.zeiss.com/microscopy/en/products/accessories/colibri.html|Zeiss Colibri 7]] | ||
| + | * **Features** | ||
| + | * | [[https://www.imdik.pan.pl/images/Us%C5%82ugi_dokumenty/Przydatne_informacje/2d_Definite_Focus_information.pdf|Definite Focus.3]] | ||
| + | * | [[https://www.youtube.com/watch?v=tUiEa7vcggQ|Airyscan 2 Multiplex]] | ||
| + | * | [[https://www.zeiss.com/microscopy/en/about-us/newsroom/press-releases/2021/zeiss-lsm-plus-and-zeiss-airyscan-joint-deconvolution.html|LSM Plus]] | ||
| + | * | [[https://www.zeiss.com/microscopy/en/about-us/newsroom/press-releases/2021/zeiss-lsm-plus-and-zeiss-airyscan-joint-deconvolution.html|Joint Deconvolution]] | ||
| <html> | <html> | ||
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| </html> | </html> | ||
| - | {{:warning.png?25|}} Data files older than **1 month** will be automatically deleted on this system, please copy your data to the iMM server using the desktop link. | + | * [[https://www.youtube.com/watch?v=zA4xqq-EL9g|ZEISS Webinar: LSM 980 Airyscan 2 Multiplex mode]] |
| - | ===== Additional information ===== | + | {{:warning.png?25|}} Data files older than **1 month** will be automatically deleted on this system, please copy your data to the iMM server using the desktop link. |
| - | + | ||
| - | * [[https://www.youtube.com/watch?v=zA4xqq-EL9g|ZEISS Webinar: Exploring the advancements in fast and gentle super-resolution imaging with LSM 980 Airyscan 2 Multiplex mode (video)]] | + | |
| ===== Booking Rules ===== | ===== Booking Rules ===== | ||
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| | 40x | LD C-Apochromat Corr | Water | 1.10 | 0.62 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/421867-9970-000/Objective-LD-C-Apochromat-40x-1.1-W-Corr-M27|421867-9970-000]] | | | 40x | LD C-Apochromat Corr | Water | 1.10 | 0.62 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/421867-9970-000/Objective-LD-C-Apochromat-40x-1.1-W-Corr-M27|421867-9970-000]] | | ||
| | 63x | Plan-Apochromat DIC | Oil | 1.40 | 0.19 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420782-9900-799/Objective-Plan-Apochromat-63x-1.4-Oil-DIC-M27|420782-9900-799]] | | | 63x | Plan-Apochromat DIC | Oil | 1.40 | 0.19 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420782-9900-799/Objective-Plan-Apochromat-63x-1.4-Oil-DIC-M27|420782-9900-799]] | | ||
| - | ==== Filtersets (Ocular) ==== | + | |
| + | ==== LEDs (Ocular) ==== | ||
| + | ^ Line ^ Wavelength ^ | ||
| + | | UV | 385 nm | | ||
| + | | B | 475 nm | | ||
| + | | G | 555 nm | | ||
| + | | R | 630 nm | | ||
| + | |||
| + | ==== Filterset (Ocular) ==== | ||
| ^ Position ^ Filterset ^ Reference ^ Excitation ^ Dichroic ^ Emission ^ | ^ Position ^ Filterset ^ Reference ^ Excitation ^ Dichroic ^ Emission ^ | ||
| - | | 1 | Green | [[https://www.micro-shop.zeiss.com/?s=7139069fdc79c&l=en&p=us&f=f&a=v&b=f&id=489038-9901-000&o=|FS38HE]] | 450-490 nm | 495 nm | 500-550 nm | | + | | 1 | QUAD | [[https://www.micro-shop.zeiss.com/en/us/shop/filterAssistant/filtersets/489090-9100-000| FS90HE]] | 370-400 nm | 405 nm | 410-440 nm | |
| - | | 2 | Red | [[https://www.micro-shop.zeiss.com/?s=163176628fcf844&l=en&p=us&f=f&a=v&b=f&id=000000-1114-101&o=|FS43]] | 533-558 nm | 570 nm | 570-640 nm | | + | | ::: | ::: | ::: | 450-488 nm | 493 nm | 499-529 nm | |
| - | | 3 | Blue | [[https://www.micro-shop.zeiss.com/?s=163176628fcf844&l=en&p=us&f=f&a=v&b=f&id=488049-9901-000&o=|FS49]] | G 365 nm | 395 nm | 420-470 nm | | + | | ::: | ::: | ::: | 540-570 nm | 575 nm | 580-605 nm | |
| + | | ::: | ::: | ::: | 615-648 nm | 653 nm | 659-759 nm | | ||
| ===== Microscope Turn On Procedure ===== | ===== Microscope Turn On Procedure ===== | ||
| + | |||
| + | The system should be always on, you should only need to turn on the computer, log into Windows and start **ZEN**. | ||
| + | |||
| + | If the system is completely off (e.g. during weekends), please follow this procedure: | ||
| + | |||
| * Turn on the ''MAIN SWITCH'' | * Turn on the ''MAIN SWITCH'' | ||
| - | {{zeiss_lsm_880_1.jpg?100}} | + | {{::zeiss_lsm_980_main_switch.png?100|}} |
| - | * Turn on the ''SYSTEMS/PC'' switch | + | * Turn on the ''Laser'' key |
| + | {{::zeiss_lsm_980_laser_key.png?100|}} | ||
| * Turn on the computer | * Turn on the computer | ||
| - | * Login in to Windows (Bioimaging User) | + | * Log into Windows with your Agendo credentials |
| * Turn on the ''COMPONENTS'' switch | * Turn on the ''COMPONENTS'' switch | ||
| - | * Start the **ZEN Black** software | + | {{::zeiss_lsm_980_components.png?100|}} |
| + | * Start the **ZEN** software | ||
| ===== Microscope Turn Off Procedure ===== | ===== Microscope Turn Off Procedure ===== | ||
| - | If there is another user for this microscope in the __next hour__: | + | |
| - | * Exit the ZEN software, leave the lasers on | + | Please do not shutdown the system. Just exit the **ZEN** software, log off Windows and shutdown the computer. |
| - | * Log off the computer | + | Don't forget to clean immersion objectives! |
| - | * Clean up immersion objectives | + | |
| - | Else: | + | |
| - | * Turn off the lasers on **ZEN** | + | |
| - | * Clean up immersion objectives | + | |
| - | * Exit the ZEN software | + | |
| - | * Shutdown the computer | + | |
| - | * Turn off the two switches ''SYSTEMS/PC'' and ''COMPONENTS'' | + | |
| - | * **Wait 5 min. for Ar laser to cool down** | + | |
| - | * Turn off the ''MAIN SWITCH'' | + | |
| ---- | ---- | ||
| [[resources|Back to the Equipment page]] | [[resources|Back to the Equipment page]] | ||
zeiss_lsm_980.1662070956.txt.gz · Last modified: 2022/09/02 00:22 by bioimaging
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