zeiss_lsm_880
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zeiss_lsm_880 [2020/07/21 23:22] bioimaging |
zeiss_lsm_880 [2024/01/28 11:47] (current) bioimaging [Microscope overview] |
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| - | <a href="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_lsm_880_wiki_new.png"><img src="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_lsm_880_wiki_new.png" width=300></a> | + | <a href="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_lsm_880_wiki_2022.png"><img src="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_lsm_880_wiki_2022.png" width=300></a> |
| <td style="border:0px solid white;"> <p style="line-height:1.8"> | <td style="border:0px solid white;"> <p style="line-height:1.8"> | ||
| <b>Location</b>: <a href="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_lsm_880_map_new.png">Room P2-A-22</a> (<img src="/facility/bioimaging/lib/exe/fetch.php?media=phone_neg.png" width=15> 47217) <br> | <b>Location</b>: <a href="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_lsm_880_map_new.png">Room P2-A-22</a> (<img src="/facility/bioimaging/lib/exe/fetch.php?media=phone_neg.png" width=15> 47217) <br> | ||
| - | <b>Manufacturer</b>: <a href="http://www.zeiss.de/micro" target="_blank">Carl Zeiss MicroImaging</a><br> | + | <b>Manufacturer</b>: <a href="http://www.zeiss.de/micro" target="_blank">ZEISS Microscopy</a><br> |
| - | <b>Model</b>: LSM 880 with Airyscan<br> | + | <b>Model</b>: <a href="https://www.youtube.com/watch?v=sGTNBXq8omg">LSM 880 with Airyscan</a> (click for Zeiss webinar) <br> |
| <b>Nickname</b>: "880"<br> | <b>Nickname</b>: "880"<br> | ||
| - | <b>Software</b>: ZEN 2.1 (black) <br> | + | <b>Software</b>: ZEN 2.3 SP1 (black edition) <br> |
| <b>Year</b>: 2016 <br> | <b>Year</b>: 2016 <br> | ||
| <b>SN</b>: 2811000267 <br> | <b>SN</b>: 2811000267 <br> | ||
| + |   <br> | ||
| + | Data will be deleted after: <b>1 month</b> | ||
|   <br> |   <br> | ||
|   <br> |   <br> | ||
|   <br> |   <br> | ||
| - | → <a href="https://my.agendo.science/calendar/?id=OGNhbGVuZGFycw=="><img src="/facility/bioimaging/lib/exe/fetch.php?media=date.png"> Zeiss LSM 880 Booking</a> | ||
| - | <br> | ||
| → <a href="/facility/bioimaging/doku.php?id=zeiss_lsm_880_quality"><img src="/facility/bioimaging/lib/exe/fetch.php?media=list-icon.png" width=18> Zeiss LSM 880 Quality Control</a> | → <a href="/facility/bioimaging/doku.php?id=zeiss_lsm_880_quality"><img src="/facility/bioimaging/lib/exe/fetch.php?media=list-icon.png" width=18> Zeiss LSM 880 Quality Control</a> | ||
| <br> | <br> | ||
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| ===== Microscope overview ===== | ===== Microscope overview ===== | ||
| - | {{ :airyscan_beampath.png?400}} The Zeiss LSM 880 with Airyscan is a //confocal point-scanning microscope// able to generate high-resolution three-dimensional images of thick specimens with high sensitivity, high speed and low photodamage. It is an inverted microscope especially suitable for live cell imaging and photobleaching experiments, equipped with a large size incubator for temperature control and CO2 supply and with Definite Focus for hardware focus control during long time-lapse acquisitions. The stage is motorized and furthermore equipped with a piezo for Z displacement so fast 4D imaging is possible in multiple stage positions. Its [[http://www.zeiss.com/content/dam/Microscopy/Products/laser-scan/LSM%20880/zeiss-lsm880-airyscan-principle-672.jpg|Airyscan]] 32 channel area detector is able to collect all light from an Airy pattern simultaneously, allowing for increased resolution (140 nm laterally and 400 nm axially, at 488 nm). It can also be used in [[https://www.youtube.com/watch?v=ln6VUmLzLLc|Fast Mode]], which enables parallel excitation and detection of four image pixels. The result is a speed improvement by a factor of four, while maintaining the sensitivity of Airyscan and 1.5x resolution improvement. Its scanning unit further includes two PMT detectors (one of them cooled) as well as a GaAsP detector for increased sensitivity (45% QE compared to ~25% QE for conventional PMT). It is equipped with lasers from violet to far red (405, 458, 488, 514, 561, 594 and 633 nm excitation wavelengths). | + | {{ :confocal_pinhole.png?340}} The Zeiss LSM 880 with Airyscan is a [[https://www.youtube.com/watch?v=rTkjQ6Q0p5I|point-scanning confocal microscope]] able to generate high-resolution three-dimensional images of thick specimens with high sensitivity, high speed and low photodamage. It is an inverted microscope especially suitable for live cell imaging and photobleaching experiments, equipped with a large cage incubator and a stage top incubator for temperature control and CO2 supply and with Definite Focus for hardware focus control during long time-lapse acquisitions. The stage is motorized and furthermore equipped with a piezo for Z displacement so fast 4D imaging is possible in multiple stage positions. Its [[http://www.zeiss.com/content/dam/Microscopy/Products/laser-scan/LSM%20880/zeiss-lsm880-airyscan-principle-672.jpg|Airyscan]] with 32 concentrically arranged GaAsP detection elements is able to collect all light from an Airy pattern simultaneously, allowing for increased resolution (140 nm laterally and 400 nm axially, at 488 nm). It can also be used in [[https://www.youtube.com/watch?v=ln6VUmLzLLc|Fast Mode]], which enables parallel excitation and detection of four image pixels. The result is a speed improvement by a factor 4x, while maintaining the sensitivity of Airyscan and 1.5x resolution improvement. Its scanning unit further includes two PMT detectors (one of them cooled) as well as a GaAsP detector for increased sensitivity (45% QE compared to ~25% QE for conventional PMT). It is equipped with lasers from violet to far red (405, 458, 488, 514, 561, 594 and 633 nm excitation wavelengths). |
| - | {{::fast_airyscan.png?420 }}With this system you can perform optical sectioning of fluorescent samples which are too thick for a widefield system such as the [[zeiss_cell_observer|Zeiss Cell Observer]] or the [[zeiss_axiovert_200m|Zeiss Axiovert 200M]]. Image resolution with Airyscan is higher than the [[zeiss_lsm_710|Zeiss LSM 710]] and detection sensitivity is also higher than the spinning disks [[3i_marianas_sdc|3i Marianas SDC]] and [[zeiss_cell_observer_sd|Zeiss Cell Observer SD]]. If your personal computer cannot handle all the data you collected, check out the [[big_guy|Big Guy]] or [[colossus|Colossus]]. | + | |
| - | {{:info.png?25|}} Click [[https://imm.medicina.ulisboa.pt/facility/bioimaging/lib/exe/fetch.php?cache=&media=airyscan.jpg|here]] to see the system beam path in higher detail | + | * [[https://www.youtube.com/watch?v=sGTNBXq8omg|ZEISS Webinar: LSM 880 with Airyscan - Revolutionize Your Confocal Imaging]] |
| + | * [[https://www.youtube.com/watch?v=VuxgaKDa_DU|ZEISS Webinar: Fast Mode for ZEISS LSM 880 with Airyscan]] | ||
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| + | <html> | ||
| + | <p align="center"><iframe width="420" height="236" src="https://www.youtube.com/embed/QjKrLTHGnc8" title="YouTube video player" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture" allowfullscreen></iframe> <iframe width="420" height="236" src="https://www.youtube.com/embed/ln6VUmLzLLc" title="YouTube video player" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture" allowfullscreen></iframe></p> | ||
| + | </html> | ||
| {{:warning.png?25|}} Data files older than **1 month** will be automatically deleted on this system, please copy your data to the iMM server using the desktop link. | {{:warning.png?25|}} Data files older than **1 month** will be automatically deleted on this system, please copy your data to the iMM server using the desktop link. | ||
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| ==== LASERs ==== | ==== LASERs ==== | ||
| - | ^ Laser Unit ^ Wavelength ^ Maximum Power ^ Current Status ^ | + | ^ Laser Unit ^ Wavelength ^ Maximum Power ^ |
| - | | Diode 405-30 | 405 nm | 30 mW | ok | | + | | Diode 405-30 | 405 nm | 30 mW | |
| - | | Argon | 458, 488 and 514 nm | 25 mW | ok | | + | | Argon | 458, 488 and 514 nm | 25 mW | |
| - | | DPSS 561-20 | 561 nm | 20 mW | ok | | + | | DPSS 561-20 | 561 nm | 20 mW | |
| - | | HeNe594 | 594 nm | 2 mW | ok | | + | | HeNe594 | 594 nm | 2 mW | |
| - | | HeNe633 | 633 nm | 5 mW | ok | | + | | HeNe633 | 633 nm | 5 mW | |
| ==== Objectives ==== | ==== Objectives ==== | ||
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| | 20x | Plan-Apochromat | Air | 0.80 | 0.55 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420650-9901-000/Objective-Plan-Apochromat-20x-0.8-M27|420650-9901-000]] | | | 20x | Plan-Apochromat | Air | 0.80 | 0.55 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420650-9901-000/Objective-Plan-Apochromat-20x-0.8-M27|420650-9901-000]] | | ||
| | 40x | C-Apochromat Corr | Water | 1.20 | 0.28 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/421767-9970-000/Objective-C-Apochromat-40x-1.20-W-Corr-M27|421767-9970-000]] | | | 40x | C-Apochromat Corr | Water | 1.20 | 0.28 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/421767-9970-000/Objective-C-Apochromat-40x-1.20-W-Corr-M27|421767-9970-000]] | | ||
| + | | 40x | LD C-Apochromat Corr | Water | 1.10 | 0.62 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/421867-9970-000/Objective-LD-C-Apochromat-40x-1.1-W-Corr-M27|421867-9970-000]] | | ||
| | 63x | Plan-Apochromat DIC | Oil | 1.40 | 0.19 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420782-9900-799/Objective-Plan-Apochromat-63x-1.4-Oil-DIC-M27|420782-9900-799]] | | | 63x | Plan-Apochromat DIC | Oil | 1.40 | 0.19 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420782-9900-799/Objective-Plan-Apochromat-63x-1.4-Oil-DIC-M27|420782-9900-799]] | | ||
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| | 25x | LCI Plan-Neofluar Corr DIC | Oil/Glyc/W | 0.80 | 0.21 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420852-9973-000/Objective-LCI-Plan-Neofluar-25x-0.8-Imm-Corr-DIC-M27|420852-9972-000]] | | | 25x | LCI Plan-Neofluar Corr DIC | Oil/Glyc/W | 0.80 | 0.21 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420852-9973-000/Objective-LCI-Plan-Neofluar-25x-0.8-Imm-Corr-DIC-M27|420852-9972-000]] | | ||
| | 40x | Plan-Apochromat Corr | Dry | 0.95 | 0.25 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420660-9970-000/Objective-Plan-Apochromat-40x-0.95-Corr-M27|420660-9970-000]] | | | 40x | Plan-Apochromat Corr | Dry | 0.95 | 0.25 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420660-9970-000/Objective-Plan-Apochromat-40x-0.95-Corr-M27|420660-9970-000]] | | ||
| - | | 100x | α Plan-Apochromat DIC | Oil | 1.46 | 0.11 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420792-9800-000/Objective-alpha-Plan-Apochromat-100x-1.46-Oil-DIC-M27|420792-9800-000]] | | ||
| ==== Filtersets (Ocular) ==== | ==== Filtersets (Ocular) ==== | ||
| ^ Position ^ Filterset ^ Reference ^ Excitation ^ Dichroic ^ Emission ^ | ^ Position ^ Filterset ^ Reference ^ Excitation ^ Dichroic ^ Emission ^ | ||
| - | | 1 | Green | [[https://www.micro-shop.zeiss.com/?s=7139069fdc79c&l=en&p=us&f=f&a=v&b=f&id=489038-9901-000&o=|FS38HE]] | 450-490 nm | 495 nm | 500-550 nm | | + | | 2 | Cyan | [[https://www.chroma.com/products/sets/49001-et-ecfp|49001]] | 426-446 nm | 455 nm | 460-500 nm | |
| - | | 2 | Red | [[https://www.micro-shop.zeiss.com/?s=163176628fcf844&l=en&p=us&f=f&a=v&b=f&id=000000-1114-101&o=|FS43]] | 533-558 nm | 570 nm | 570-640 nm | | + | | 3 | Green | [[https://www.micro-shop.zeiss.com/?s=7139069fdc79c&l=en&p=us&f=f&a=v&b=f&id=489038-9901-000&o=|FS38HE]] | 450-490 nm | 495 nm | 500-550 nm | |
| - | | 3 | Blue | [[https://www.micro-shop.zeiss.com/?s=163176628fcf844&l=en&p=us&f=f&a=v&b=f&id=488049-9901-000&o=|FS49]] | G 365 nm | 395 nm | 420-470 nm | | + | | 4 | Red | [[https://www.micro-shop.zeiss.com/?s=163176628fcf844&l=en&p=us&f=f&a=v&b=f&id=000000-1114-101&o=|FS43]] | 533-558 nm | 570 nm | 570-640 nm | |
| + | | 5 | Blue | [[https://www.micro-shop.zeiss.com/?s=163176628fcf844&l=en&p=us&f=f&a=v&b=f&id=488049-9901-000&o=|FS49]] | G 365 nm | 395 nm | 420-470 nm | | ||
| ===== Microscope Turn On Procedure ===== | ===== Microscope Turn On Procedure ===== | ||
| * Turn on the ''MAIN SWITCH'' | * Turn on the ''MAIN SWITCH'' | ||
| - | * Turn on the two switches: ''SYSTEMS/PC'' and ''COMPONENTS'' | ||
| {{zeiss_lsm_880_1.jpg?100}} | {{zeiss_lsm_880_1.jpg?100}} | ||
| + | * Turn on the ''SYSTEMS/PC'' switch | ||
| * Turn on the computer | * Turn on the computer | ||
| - | * Login in to Windows (Bioimaging User) | + | * Login in to Windows with your Agendo credentials |
| + | * Turn on the ''COMPONENTS'' switch | ||
| * Start the **ZEN Black** software | * Start the **ZEN Black** software | ||
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| ---- | ---- | ||
| - | [[resources|Back to the Resources page]] | + | [[resources|Back to the Equipment page]] |
zeiss_lsm_880.1595366526.txt.gz · Last modified: 2020/07/21 23:22 by bioimaging
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