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zeiss_lsm_880 [2020/07/21 23:22]
bioimaging
zeiss_lsm_880 [2021/01/17 04:43] (current)
bioimaging [Microscope Turn Off Procedure]
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 <​b>​Location</​b>:​ <a href="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=zeiss_lsm_880_map_new.png">​Room P2-A-22</​a>​ (<img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=phone_neg.png"​ width=15>​ 47217) <​br> ​ <​b>​Location</​b>:​ <a href="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=zeiss_lsm_880_map_new.png">​Room P2-A-22</​a>​ (<img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=phone_neg.png"​ width=15>​ 47217) <​br> ​
 <​b>​Manufacturer</​b>:​ <a href="​http://​www.zeiss.de/​micro"​ target="​_blank">​Carl Zeiss MicroImaging</​a><​br>​ <​b>​Manufacturer</​b>:​ <a href="​http://​www.zeiss.de/​micro"​ target="​_blank">​Carl Zeiss MicroImaging</​a><​br>​
-<​b>​Model</​b>:​ LSM 880 with Airyscan<​br>​+<​b>​Model</​b>: ​<a href="​https://​www.youtube.com/​watch?​v=sGTNBXq8omg">​LSM 880 with Airyscan</a> (click for Zeiss webinar) ​<br>
 <​b>​Nickname</​b>:​ "​880"<​br>​ <​b>​Nickname</​b>:​ "​880"<​br>​
-<​b>​Software</​b>:​ ZEN 2.(black) <br>+<​b>​Software</​b>:​ ZEN 2.3 SP1 (black ​edition) <br>
 <​b>​Year</​b>:​ 2016 <br> <​b>​Year</​b>:​ 2016 <br>
 <​b>​SN</​b>:​ 2811000267 <br> <​b>​SN</​b>:​ 2811000267 <br>
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 ===== Microscope overview ===== ===== Microscope overview =====
  
-{{  :​airyscan_beampath.png?​400}} The Zeiss LSM 880 with Airyscan is a //confocal point-scanning microscope// able to generate high-resolution three-dimensional images of thick specimens with high sensitivity,​ high speed and low photodamage. It is an inverted microscope especially suitable for live cell imaging and photobleaching experiments,​ equipped with a large size incubator for temperature control and CO2 supply and with Definite Focus for hardware focus control during long time-lapse acquisitions. The stage is motorized and furthermore equipped with a piezo for Z displacement so fast 4D imaging is possible in multiple stage positions. Its [[http://​www.zeiss.com/​content/​dam/​Microscopy/​Products/​laser-scan/​LSM%20880/​zeiss-lsm880-airyscan-principle-672.jpg|Airyscan]] 32 channel area detector is able to collect all light from an Airy pattern simultaneously,​ allowing for increased resolution (140 nm laterally and 400 nm axially, at 488 nm). It can also be used in [[https://​www.youtube.com/​watch?​v=ln6VUmLzLLc|Fast Mode]], which enables parallel excitation and detection of four image pixels. The result is a speed improvement by a factor of four, while maintaining the sensitivity of Airyscan and 1.5x resolution improvement. Its scanning unit further includes two PMT detectors (one of them cooled) as well as a GaAsP detector for increased sensitivity (45% QE compared to ~25% QE for conventional PMT). It is equipped with lasers from violet to far red (405, 458, 488, 514, 561, 594 and 633 nm excitation wavelengths). ​+{{  :​airyscan_beampath.png?​400}} The Zeiss LSM 880 with Airyscan is a [[https://imm.medicina.ulisboa.pt/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=confocal.png|confocal point-scanning microscope]] able to generate high-resolution three-dimensional images of thick specimens with high sensitivity,​ high speed and low photodamage. It is an inverted microscope especially suitable for live cell imaging and photobleaching experiments,​ equipped with a large cage incubator and a stage top incubator for temperature control and CO2 supply and with Definite Focus for hardware focus control during long time-lapse acquisitions. The stage is motorized and furthermore equipped with a piezo for Z displacement so fast 4D imaging is possible in multiple stage positions. Its [[http://​www.zeiss.com/​content/​dam/​Microscopy/​Products/​laser-scan/​LSM%20880/​zeiss-lsm880-airyscan-principle-672.jpg|Airyscan]] 32 channel area detector is able to collect all light from an Airy pattern simultaneously,​ allowing for increased resolution (140 nm laterally and 400 nm axially, at 488 nm). It can also be used in [[https://​www.youtube.com/​watch?​v=ln6VUmLzLLc|Fast Mode]], which enables parallel excitation and detection of four image pixels. The result is a speed improvement by a factor of four, while maintaining the sensitivity of Airyscan and 1.5x resolution improvement. Its scanning unit further includes two PMT detectors (one of them cooled) as well as a GaAsP detector for increased sensitivity (45% QE compared to ~25% QE for conventional PMT). It is equipped with lasers from violet to far red (405, 458, 488, 514, 561, 594 and 633 nm excitation wavelengths). ​
 {{::​fast_airyscan.png?​420 ​ }}With this system you can perform optical sectioning of fluorescent samples which are too thick for a widefield system such as the [[zeiss_cell_observer|Zeiss Cell Observer]] or the [[zeiss_axiovert_200m|Zeiss Axiovert 200M]]. Image resolution with Airyscan is higher than the [[zeiss_lsm_710|Zeiss LSM 710]] and detection sensitivity is also higher than the spinning disks [[3i_marianas_sdc|3i Marianas SDC]] and [[zeiss_cell_observer_sd|Zeiss Cell Observer SD]]. If your personal computer cannot handle all the data you collected, check out the [[big_guy|Big Guy]] or [[colossus|Colossus]]. {{::​fast_airyscan.png?​420 ​ }}With this system you can perform optical sectioning of fluorescent samples which are too thick for a widefield system such as the [[zeiss_cell_observer|Zeiss Cell Observer]] or the [[zeiss_axiovert_200m|Zeiss Axiovert 200M]]. Image resolution with Airyscan is higher than the [[zeiss_lsm_710|Zeiss LSM 710]] and detection sensitivity is also higher than the spinning disks [[3i_marianas_sdc|3i Marianas SDC]] and [[zeiss_cell_observer_sd|Zeiss Cell Observer SD]]. If your personal computer cannot handle all the data you collected, check out the [[big_guy|Big Guy]] or [[colossus|Colossus]].
  
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 {{:​warning.png?​25|}} Data files older than **1 month** will be automatically deleted on this system, please copy your data to the iMM server using the desktop link. {{:​warning.png?​25|}} Data files older than **1 month** will be automatically deleted on this system, please copy your data to the iMM server using the desktop link.
 +
 +===== Additional information =====
 +
 +  * [[https://​www.youtube.com/​watch?​v=sGTNBXq8omg|ZEISS Webinar: LSM 880 with Airyscan - Revolutionize Your Confocal Imaging (video)]]
 +  * [[https://​www.youtube.com/​watch?​v=VuxgaKDa_DU|ZEISS Webinar: Fast Mode for ZEISS LSM 880 with Airyscan (video)]]
  
 ===== Booking Rules ===== ===== Booking Rules =====
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 ===== Microscope Turn On Procedure ===== ===== Microscope Turn On Procedure =====
   * Turn on the ''​MAIN SWITCH''​   * Turn on the ''​MAIN SWITCH''​
-  * Turn on the two switches: ​''​SYSTEMS/​PC'' ​and ''​COMPONENTS''​+  * Turn on the ''​SYSTEMS/​PC'' ​switch
 {{zeiss_lsm_880_1.jpg?​100}} {{zeiss_lsm_880_1.jpg?​100}}
   * Turn on the computer   * Turn on the computer
   * Login in to Windows (Bioimaging User)   * Login in to Windows (Bioimaging User)
 +  * Turn on the ''​COMPONENTS''​ switch
 +  * Check that the computer is connected to an Unidentified network (click on the network icon in the taskbar)
   * Start the **ZEN Black** software   * Start the **ZEN Black** software
 +
 +**Warning**:​ If you need to change the stage adapter, please contact the <​html><​a href="/​facility/​bioimaging/​doku.php?​id=about">​Bioimaging Unit</​a>​ (<​b>​imm-bioimaging@medicina.ulisboa.pt</​b>​ | <img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=phone_neg.png"​ width=15>​ 47222</​html>​)
  
 ===== Microscope Turn Off Procedure ===== ===== Microscope Turn Off Procedure =====
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 ---- ----
-[[resources|Back to the Resources ​page]]+[[resources|Back to the Equipment ​page]]
zeiss_lsm_880.1595366526.txt.gz ยท Last modified: 2020/07/21 23:22 by bioimaging