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zeiss_lsm_880 [2016/06/08 17:28]
bioimaging [Objectives]
zeiss_lsm_880 [2024/01/28 11:47] (current)
bioimaging [Microscope overview]
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-<img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=zeiss_lsm_880_name.jpg">+<img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=zeiss_lsm_880_name_new.png">
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-<a href="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=zeiss_lsm_880_wiki.jpg"><​img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=zeiss_lsm_880_wiki.jpg" width=300></​a>​+<a href="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=zeiss_lsm_880_wiki_2022.png"><​img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=zeiss_lsm_880_wiki_2022.png" width=300></​a>​
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-<​b>​Location</​b>:​ <a href="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=zeiss_lsm_880_map.jpg">​Room P2-A-22</​a>​ (<img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=telephone_icon.gif" width=15>​ 47217) <br>  +<​b>​Location</​b>:​ <a href="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=zeiss_lsm_880_map_new.png">​Room P2-A-22</​a>​ (<img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=phone_neg.png" width=15>​ 47217) <br>  
-<​b>​Manufacturer</​b>:​ <a href="​http://​www.zeiss.de/​micro"​ target="​_blank">​Carl Zeiss MicroImaging</​a><​br>​ +<​b>​Manufacturer</​b>:​ <a href="​http://​www.zeiss.de/​micro"​ target="​_blank">​ZEISS Microscopy</​a><​br>​ 
-<​b>​Model</​b>:​ <a href="http://www.zeiss.com/microscopy/​en_de/​products/​confocal-microscopes/​lsm-880-with-airyscan-.html"​ target="​_blank">​LSM 880 with Airyscan</​a><​br>​ +<​b>​Model</​b>:​ <a href="https://www.youtube.com/watch?v=sGTNBXq8omg">​LSM 880 with Airyscan</​a> ​(click for Zeiss webinar) <​br>​ 
-<​b>​Software</​b>:​ ZEN 2.(black) <br>+<​b>​Nickname</​b>:​ "​880"​<​br>​ 
 +<​b>​Software</​b>:​ ZEN 2.3 SP1 (black ​edition) <br>
 <​b>​Year</​b>:​ 2016 <br> <​b>​Year</​b>:​ 2016 <br>
 <​b>​SN</​b>:​ 2811000267 <br> <​b>​SN</​b>:​ 2811000267 <br>
 &nbsp <br> &nbsp <br>
 +Data will be deleted after: <b>1 month</​b>​
 &nbsp <br> &nbsp <br>
 &nbsp <br> &nbsp <br>
 &nbsp <br> &nbsp <br>
-&rarr; <a href="​https://​imm.medicina.ulisboa.pt/​intranet/​booking/​128"><​img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=date.png"> ​ Zeiss LSM 880 Booking</​a>​ 
- <​br> ​ 
 &rarr; <a href="/​facility/​bioimaging/​doku.php?​id=zeiss_lsm_880_quality"><​img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=list-icon.png"​ width=18>​ Zeiss LSM 880 Quality Control</​a>​ &rarr; <a href="/​facility/​bioimaging/​doku.php?​id=zeiss_lsm_880_quality"><​img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=list-icon.png"​ width=18>​ Zeiss LSM 880 Quality Control</​a>​
  <​br>​  <​br>​
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 ===== Microscope overview ===== ===== Microscope overview =====
  
-{{  :airyscan.jpg?0x380}} The Zeiss LSM 880 with Airyscan is a //confocal ​point-scanning microscope// able to generate high-resolution three-dimensional images of thick specimens with high sensitivity and low photodamage. It is an inverted microscope ​specially ​suitable for live cell imaging and photobleaching experiments,​ equipped with a large size incubator for temperature control and CO2 supply and with Definite Focus for hardware focus control during long time-lapse acquisitions. Its [[http://​www.zeiss.com/​content/​dam/​Microscopy/​Products/​laser-scan/​LSM%20880/​zeiss-lsm880-airyscan-principle-672.jpg|Airyscan]] 32 channel area detector ​is able to collect all light from an Airy pattern simultaneously,​ allowing for increased resolution (140 nm laterally and 400 nm axially, at 488 nm). Its scanning unit further includes two PMT detectors (one of them cooled) as well as a GaAsP detector for increased sensitivity (45% QE compared to ~25% QE for conventional PMT).  It is equipped with lasers from violet to far red (405, 458, 488, 514, 561, 594 and 633 nm excitation wavelengths). With this system you can perform optical sectioning of fluorescent samples which are too thick for a widefield system such as the [[zeiss_cell_observer_wf|Zeiss Cell Observer WF]] or the [[zeiss_axiovert_200m|Zeiss Axiovert 200M]]. Image resolution with Airyscan is higher than the [[zeiss_lsm_710|Zeiss LSM 710]] and detection sensitivity is also higher than faster confocal systems such as the spinning disk [[3i_marianas_sdc|3i Marianas SDC]] or the //line scanning// [[zeiss_lsm_7_live|Zeiss LSM 7 Live]]. If your personal computer cannot handle all the data you collected, check out the [[big_guy|Big Guy]] or [[colossus|Colossus]].+{{  :confocal_pinhole.png?340}} The Zeiss LSM 880 with Airyscan is a [[https://www.youtube.com/​watch?​v=rTkjQ6Q0p5I|point-scanning ​confocal ​microscope]] able to generate high-resolution three-dimensional images of thick specimens with high sensitivity, high speed and low photodamage. It is an inverted microscope ​especially ​suitable for live cell imaging and photobleaching experiments,​ equipped with a large cage incubator and a stage top incubator for temperature control and CO2 supply and with Definite Focus for hardware focus control during long time-lapse acquisitions. The stage is motorized and furthermore equipped with a piezo for Z displacement so fast 4D imaging is possible in multiple stage positions. Its [[http://​www.zeiss.com/​content/​dam/​Microscopy/​Products/​laser-scan/​LSM%20880/​zeiss-lsm880-airyscan-principle-672.jpg|Airyscan]] ​with 32 concentrically arranged GaAsP detection elements ​is able to collect all light from an Airy pattern simultaneously,​ allowing for increased resolution (140 nm laterally and 400 nm axially, at 488 nm). It can also be used in [[https://​www.youtube.com/​watch?​v=ln6VUmLzLLc|Fast Mode]], which enables parallel excitation and detection of four image pixels. The result is a speed improvement by a factor 4x, while maintaining the sensitivity of Airyscan and 1.5x resolution improvement. Its scanning unit further includes two PMT detectors (one of them cooled) as well as a GaAsP detector for increased sensitivity (45% QE compared to ~25% QE for conventional PMT). It is equipped with lasers from violet to far red (405, 458, 488, 514, 561, 594 and 633 nm excitation wavelengths).
  
-{{info.gif?|}} Click on the image on the left to see the system beam path in higher detail+  * [[https://​www.youtube.com/​watch?​v=sGTNBXq8omg|ZEISS Webinar: LSM 880 with Airyscan - Revolutionize Your Confocal Imaging]] 
 +  * [[https://​www.youtube.com/​watch?v=VuxgaKDa_DU|ZEISS Webinar: Fast Mode for ZEISS LSM 880 with Airyscan]]
  
-===== Booking Rules ===== +<​html>​ 
-The Zeiss LSM 710 booking is divided ​in three daily slots: +<p align="​center"><​iframe width="​420"​ height="​236"​ src="​https://​www.youtube.com/​embed/​QjKrLTHGnc8"​ title="​YouTube video player"​ frameborder="​0"​ allow="​accelerometer;​ autoplay; clipboard-write;​ encrypted-media;​ gyroscope; picture-in-picture"​ allowfullscreen></​iframe>​ <iframe width="​420"​ height="​236"​ src="​https://​www.youtube.com/​embed/​ln6VUmLzLLc"​ title="​YouTube video player"​ frameborder="​0"​ allow="​accelerometer;​ autoplay; clipboard-write;​ encrypted-media;​ gyroscope; picture-in-picture"​ allowfullscreen></​iframe></​p>​ 
-|  9:00 to 13:00  |  13:00 to 17:00  |  17:00 to 21:00  |+</​html>​
  
-  ​**user** can book at maximum **2 sessions ​per week** +{{:​warning.png?​25|}} Data files older than **1 month** will be automatically deleted on this system, please copy your data to the iMM server using the desktop link. 
-  - Each **session must fit inside one of the three daily slots** (i.e. a session booked from 11:00 to 14:00 counts as 2 sessions) + 
-  - The above rules do not apply for **weekends** and for work days **before 9:00** and **after 21:00** +===== Booking Rules ===== 
-  - The above rules do not apply for sessions booked on the very same day or **24 hours ahead**+  ​- **Users** can book at maximum **8 hours per week** 
 +  - This usage restriction does not apply for **weekends** and for working ​days **before 9:00** and **after 21:00**
   - Exceptions to these rules require **approval from [[joserino@medicina.ulisboa.pt|José Rino]]**.   - Exceptions to these rules require **approval from [[joserino@medicina.ulisboa.pt|José Rino]]**.
-  - Users must indicate which lasers they are going to use in the booking Description field 
-  - Users must indicate if they will use the incubator in the booking Description field (write down 37C INCUBATOR) 
-  - Users must write their internal extension phone number in the booking Description field 
  
 ===== System components ===== ===== System components =====
  
 ==== LASERs ==== ==== LASERs ====
-^  Laser Unit  ^  Wavelength ​ ^  Maximum Power  ​^ ​ Current Status ​ ^ +^  Laser Unit  ^  Wavelength ​ ^  Maximum Power  ^ 
-|  Diode 405-30 ​ |  405 nm  |  30 mW  ​| ​ ok  | +|  Diode 405-30 ​ |  405 nm  |  30 mW  | 
-|  Argon  |  458, 488 and 514 nm  |  25 mW  ​| ​ ok  | +|  Argon  |  458, 488 and 514 nm  |  25 mW  | 
-|  DPSS 561-20 ​ |  561 nm  |  20 mW  ​| ​ ok  | +|  DPSS 561-20 ​ |  561 nm  |  20 mW  | 
-|  HeNe594 ​ |  594 nm  |  2 mW  ​| ​ ok  | +|  HeNe594 ​ |  594 nm  |  2 mW  | 
-|  HeNe633 ​ |  633 nm  |  5 mW  ​| ​ ok  |+|  HeNe633 ​ |  633 nm  |  5 mW  |
  
 ==== Objectives ==== ==== Objectives ====
-^  Magnification ​  ​^ ​ Model  ^  ​Type  ​^ ​ NA  ^  WD (mm)  ^ +^  Magnification ​  ​^ ​ Model  ^  ​Immersion ​ ​^ ​ NA  ^  WD (mm)  ​^ ​ Reference ​ ^ 
-|  10x  |  [[https://​www.micro-shop.zeiss.com/​?​s=3407786617d462&​l=en&​p=uk&​f=o&​a=v&​m=s&​id=420340-9901-000|EC Plan-Neofluar]] ​ |  ​Dry  ​|  ​0.30  ​|  ​5. +|  10x  ​| ​ EC Plan-Neofluar ​ |  Air  |  0.30  |  5.20  ​| ​ [[https://​www.micro-shop.zeiss.com/​en/​de/​shop/​objectives/​420340-9901-000/Objective-EC-Plan-Neofluar-10x-0.30-M27|420340-9901-000]]  ​
-|  ​20x  ​| ​ [[https://​www.micro-shop.zeiss.com/​?​s=3407786617d462&​l=en&​p=uk&​f=o&​a=v&​m=s&​id=420650-9901-000|Plan-Apochromat]] ​ |  ​Dry  ​|  ​0.80  ​|  ​0.55  +|  ​20x  ​|  ​Plan-Apochromat ​ ​|  ​Air  |  0.80  ​|  ​0.55  ​| ​ [[https://​www.micro-shop.zeiss.com/​en/​de/​shop/​objectives/​420650-9901-000/Objective-Plan-Apochromat-20x-0.8-M27|420650-9901-000]]  ​
-|  ​25x  ​| ​ [[https://​www.micro-shop.zeiss.com/​?​s=129353259d52f12&​l=en&​p=uk&​f=o&​a=v&​m=s&​id=420852-9972-000|LCI Plan-NeoFluar]]  |  ​Oil/​Glyc/​W ​ ​|  ​0.8  ​|  ​0.21  +|  ​40x  ​|  ​C-Apochromat Corr  ​|  ​Water  |  1.20  ​|  ​0.28  ​| ​ [[https://​www.micro-shop.zeiss.com/​en/​de/​shop/​objectives/​421767-9970-000/​Objective-C-Apochromat-40x-1.20-W-Corr-M27|421767-9970-000]]  ​
-|  ​40x  ​| ​ [[https://​www.micro-shop.zeiss.com/​?​s=3407786617d462&​l=en&​p=uk&​f=o&​a=v&​m=s&​id=421767-9970-000|C-Apochromat]] ​ |  ​Water  ​|  ​1.20  ​|  ​0.28  +|  ​40x  ​|  ​LD C-Apochromat Corr  ​|  ​Water  |  1.10  ​|  ​0.62  ​| ​ [[https://​www.micro-shop.zeiss.com/​en/​de/​shop/​objectives/​421867-9970-000/​Objective-LD-C-Apochromat-40x-1.1-W-Corr-M27|421867-9970-000]]  ​
-|  ​63x  ​| ​ [[https://​www.micro-shop.zeiss.com/​?​s=3407786617d462&​l=en&​p=uk&​f=o&​a=v&​m=s&​id=440762-9904-000|Plan-Apochromat]]  |  Oil  |  ​1.40  ​ ​0.19 ​ |+|  ​63x  ​|  ​Plan-Apochromat DIC  ​|  ​Oil  |  1.40  ​|  ​0.19  ​| ​ [[https://​www.micro-shop.zeiss.com/​en/​de/​shop/​objectives/​420782-9900-799/​Objective-Plan-Apochromat-63x-1.4-Oil-DIC-M27|420782-9900-799]] ​ |
  
-**Upon request:** 
  
-^  Magnification ​  ​^ ​ Model  ^  Type  ^  NA  ^  WD (mm)  ^ +**Upon request:**
-|  40x  |  [[https://​www.micro-shop.zeiss.com/?​s=129353259d52f12&​l=en&​p=uk&​f=o&​a=v&​m=s&​id=420660-9970-000|Plan-Apochromat]] ​ |  Dry  |  0.95  |  0.25  | +
-|  100x  |  [[https://​www.micro-shop.zeiss.com/?​s=7138707e1a1de&​l=en&​p=uk&​f=o&​a=v&​m=s&​id=420792-9800-000|alpha Plan-Apochromat]] ​ |  Oil  |  1.46  |  0.11  |+
  
 +^  Magnification ​  ​^ ​ Model  ^  Immersion ​ ^  NA  ^  WD (mm)  ^  Reference ​ ^
 +|  25x  |  LCI Plan-Neofluar Corr DIC  |  Oil/​Glyc/​W ​ |  0.80  |  0.21  |  [[https://​www.micro-shop.zeiss.com/​en/​de/​shop/​objectives/​420852-9973-000/​Objective-LCI-Plan-Neofluar-25x-0.8-Imm-Corr-DIC-M27|420852-9972-000]] ​ |
 +|  40x  |  Plan-Apochromat Corr  |  Dry  |  0.95  |  0.25  |  [[https://​www.micro-shop.zeiss.com/​en/​de/​shop/​objectives/​420660-9970-000/​Objective-Plan-Apochromat-40x-0.95-Corr-M27|420660-9970-000]] ​ |
 ==== Filtersets (Ocular) ==== ==== Filtersets (Ocular) ====
  
-^  Position ​ ^  Filterset ​ ^  Reference ​ ^  Excitation ​ ^  Emission ​ ^ +^  Position ​ ^  Filterset ​ ^  Reference ​ ^  Excitation ​ ​^ ​ Dichroic ​ ​^ ​ Emission ​ ^ 
-|  ​ ​| ​ Green  |  [[https://​www.micro-shop.zeiss.com/?​s=7139069fdc79c&​l=en&​p=us&​f=f&​a=v&​b=f&​id=489038-9901-000&​o=|FS38HE]] ​ |  450-490 nm  |  500-550 nm  | +|  ​2  |  Cyan  |  [[https://​www.chroma.com/​products/​sets/​49001-et-ecfp|49001]] ​ |  426-446 nm  |  455 nm  |  460-500 nm  | 
-|  ​ ​| ​ Red  |  [[https://​www.micro-shop.zeiss.com/?​s=163176628fcf844&​l=en&​p=us&​f=f&​a=v&​b=f&​id=000000-1114-101&​o=|FS43]] ​ |  533-558 nm  |  570-640 nm  | +|  3  ​| ​ Green  |  [[https://​www.micro-shop.zeiss.com/?​s=7139069fdc79c&​l=en&​p=us&​f=f&​a=v&​b=f&​id=489038-9901-000&​o=|FS38HE]] ​ |  450-490 ​nm  |  495 nm  |  500-550 nm  | 
-|  ​ ​| ​ Blue  |  [[https://​www.micro-shop.zeiss.com/?​s=163176628fcf844&​l=en&​p=us&​f=f&​a=v&​b=f&​id=488049-9901-000&​o=|FS49]] ​ |  G 365 nm  |  420-470 nm  |+|  ​ ​| ​ Red  |  [[https://​www.micro-shop.zeiss.com/?​s=163176628fcf844&​l=en&​p=us&​f=f&​a=v&​b=f&​id=000000-1114-101&​o=|FS43]] ​ |  533-558 ​nm  |  570 nm  |  570-640 nm  | 
 +|  ​ ​| ​ Blue  |  [[https://​www.micro-shop.zeiss.com/?​s=163176628fcf844&​l=en&​p=us&​f=f&​a=v&​b=f&​id=488049-9901-000&​o=|FS49]] ​ |  G 365 nm  |  395 nm  |  420-470 nm  |
  
 ===== Microscope Turn On Procedure ===== ===== Microscope Turn On Procedure =====
   * Turn on the ''​MAIN SWITCH''​   * Turn on the ''​MAIN SWITCH''​
-  * Turn on the two switches: ''​SYSTEMS/​PC''​ and ''​COMPONENTS''​ 
 {{zeiss_lsm_880_1.jpg?​100}} {{zeiss_lsm_880_1.jpg?​100}}
-  * Turn the computer ​on +  * Turn on the ''​SYSTEMS/​PC''​ switch 
-  * Login with your unit account +  * Turn on the computer 
-  * Wait for network icon to change to ''​connected'' ​state +  * Login in to Windows ​with your Agendo credentials 
-  * Start the **ZEN** software+  * Turn on the ''​COMPONENTS'' ​switch 
 +  * Start the **ZEN Black** software
  
 ===== Microscope Turn Off Procedure ===== ===== Microscope Turn Off Procedure =====
-If there'​s ​another user for this microscope in the next hour+If there is another user for this microscope in the __next hour__
-  * Close the software, leave the lasers on and log off the computer +  * Exit the ZEN software, leave the lasers on 
-  * Clean up any objective where you used immersion ​oil. +  * Log off the computer 
-  * **Make sure that there really is another user going to use the microscope.**+  * Clean up immersion ​objectives
 Else: Else:
   * Turn off the lasers on **ZEN**   * Turn off the lasers on **ZEN**
-  * Clean up any objective were you used immersion ​oil. +  * Clean up immersion ​objectives 
-  * Exit the software, shut down the computer. +  * Exit the ZEN software 
-  * Turn off the main switches ''​SYSTEMS/​PC''​ and ''​COMPONENTS''​ +  * Shutdown ​the computer 
-  * Wait 5 min. for Ar laser cooldown +  * Turn off the two switches ''​SYSTEMS/​PC''​ and ''​COMPONENTS''​ 
-  * Turn off the ''​MAIN SWITCH''​.+  ​* **Wait 5 min. for Ar laser to cool down** 
 +  * Turn off the ''​MAIN SWITCH''​
  
 ---- ----
-[[resources|Back to the Resources ​page]]+[[resources|Back to the Equipment ​page]]
zeiss_lsm_880.1465399686.txt.gz · Last modified: 2016/06/08 17:28 by bioimaging