zeiss_lsm_880
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zeiss_lsm_880 [2021/11/29 17:02] bioimaging [Objectives] |
zeiss_lsm_880 [2022/02/14 22:09] bioimaging |
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| ===== Microscope overview ===== | ===== Microscope overview ===== | ||
| - | {{ :airyscan_beampath.png?400}} The Zeiss LSM 880 with Airyscan is a [[https://imm.medicina.ulisboa.pt/facility/bioimaging/lib/exe/fetch.php?media=confocal.png|confocal point-scanning microscope]] able to generate high-resolution three-dimensional images of thick specimens with high sensitivity, high speed and low photodamage. It is an inverted microscope especially suitable for live cell imaging and photobleaching experiments, equipped with a large cage incubator and a stage top incubator for temperature control and CO2 supply and with Definite Focus for hardware focus control during long time-lapse acquisitions. The stage is motorized and furthermore equipped with a piezo for Z displacement so fast 4D imaging is possible in multiple stage positions. Its [[http://www.zeiss.com/content/dam/Microscopy/Products/laser-scan/LSM%20880/zeiss-lsm880-airyscan-principle-672.jpg|Airyscan]] 32 channel area detector is able to collect all light from an Airy pattern simultaneously, allowing for increased resolution (140 nm laterally and 400 nm axially, at 488 nm). It can also be used in [[https://www.youtube.com/watch?v=ln6VUmLzLLc|Fast Mode]], which enables parallel excitation and detection of four image pixels. The result is a speed improvement by a factor of four, while maintaining the sensitivity of Airyscan and 1.5x resolution improvement. Its scanning unit further includes two PMT detectors (one of them cooled) as well as a GaAsP detector for increased sensitivity (45% QE compared to ~25% QE for conventional PMT). It is equipped with lasers from violet to far red (405, 458, 488, 514, 561, 594 and 633 nm excitation wavelengths). | + | {{ :confocal_pinhole.png?350}} The Zeiss LSM 880 with Airyscan is a point-scanning confocal microscope able to generate high-resolution three-dimensional images of thick specimens with high sensitivity, high speed and low photodamage. It is an inverted microscope especially suitable for live cell imaging and photobleaching experiments, equipped with a large cage incubator and a stage top incubator for temperature control and CO2 supply and with Definite Focus for hardware focus control during long time-lapse acquisitions. The stage is motorized and furthermore equipped with a piezo for Z displacement so fast 4D imaging is possible in multiple stage positions. Its [[http://www.zeiss.com/content/dam/Microscopy/Products/laser-scan/LSM%20880/zeiss-lsm880-airyscan-principle-672.jpg|Airyscan]] 32 channel area detector is able to collect all light from an Airy pattern simultaneously, allowing for increased resolution (140 nm laterally and 400 nm axially, at 488 nm). It can also be used in [[https://www.youtube.com/watch?v=ln6VUmLzLLc|Fast Mode]], which enables parallel excitation and detection of four image pixels. The result is a speed improvement by a factor of four, while maintaining the sensitivity of Airyscan and 1.5x resolution improvement. Its scanning unit further includes two PMT detectors (one of them cooled) as well as a GaAsP detector for increased sensitivity (45% QE compared to ~25% QE for conventional PMT). It is equipped with lasers from violet to far red (405, 458, 488, 514, 561, 594 and 633 nm excitation wavelengths). With this system you can perform optical sectioning of fluorescent samples which are too thick for a widefield system such as the [[zeiss_cell_observer|Zeiss Cell Observer]]. Image resolution with Airyscan is higher than the [[zeiss_lsm_710|Zeiss LSM 710]] and detection sensitivity is also higher than the spinning disks [[3i_marianas_sdc|3i Marianas SDC]] and [[zeiss_cell_observer_sd|Zeiss Cell Observer SD]]. If your personal computer cannot handle all the data you collected, check out the [[colossus|Colossus]]. |
| - | {{::fast_airyscan.png?420 }}With this system you can perform optical sectioning of fluorescent samples which are too thick for a widefield system such as the [[zeiss_cell_observer|Zeiss Cell Observer]] or the [[zeiss_axiovert_200m|Zeiss Axiovert 200M]]. Image resolution with Airyscan is higher than the [[zeiss_lsm_710|Zeiss LSM 710]] and detection sensitivity is also higher than the spinning disks [[3i_marianas_sdc|3i Marianas SDC]] and [[zeiss_cell_observer_sd|Zeiss Cell Observer SD]]. If your personal computer cannot handle all the data you collected, check out the [[big_guy|Big Guy]] or [[colossus|Colossus]]. | + | |
| - | {{:info.png?25|}} Click [[https://imm.medicina.ulisboa.pt/facility/bioimaging/lib/exe/fetch.php?cache=&media=airyscan.jpg|here]] to see the system beam path in higher detail | + | {{ ::fast_airyscan.png?600 }} |
| + | |||
| + | {{:info.png?25|}} Click [[https://imm.medicina.ulisboa.pt/facility/bioimaging/lib/exe/fetch.php?cache=&media=airyscan.jpg|here]] to see the system beam path in detail | ||
| {{:warning.png?25|}} Data files older than **1 month** will be automatically deleted on this system, please copy your data to the iMM server using the desktop link. | {{:warning.png?25|}} Data files older than **1 month** will be automatically deleted on this system, please copy your data to the iMM server using the desktop link. | ||
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| * Turn on the ''MAIN SWITCH'' | * Turn on the ''MAIN SWITCH'' | ||
| {{zeiss_lsm_880_1.jpg?100}} | {{zeiss_lsm_880_1.jpg?100}} | ||
| - | * Turn on the incubation system (**A** on remote control) | ||
| * Turn on the ''SYSTEMS/PC'' switch | * Turn on the ''SYSTEMS/PC'' switch | ||
| * Turn on the computer | * Turn on the computer | ||
| * Login in to Windows (Bioimaging User) | * Login in to Windows (Bioimaging User) | ||
| * Turn on the ''COMPONENTS'' switch | * Turn on the ''COMPONENTS'' switch | ||
| - | * Check that the computer is connected to an Unidentified network (click on the network icon in the taskbar) | ||
| * Start the **ZEN Black** software | * Start the **ZEN Black** software | ||
| - | |||
| - | **Warning**: If you need to change the stage adapter, please contact the <html><a href="/facility/bioimaging/doku.php?id=about">Bioimaging Unit</a> (<b>imm-bioimaging@medicina.ulisboa.pt</b> | <img src="/facility/bioimaging/lib/exe/fetch.php?media=phone_neg.png" width=15> 47222</html>) | ||
| ===== Microscope Turn Off Procedure ===== | ===== Microscope Turn Off Procedure ===== | ||
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| * Shutdown the computer | * Shutdown the computer | ||
| * Turn off the two switches ''SYSTEMS/PC'' and ''COMPONENTS'' | * Turn off the two switches ''SYSTEMS/PC'' and ''COMPONENTS'' | ||
| - | * Turn off the incubation system (**A** on remote control) | ||
| * **Wait 5 min. for Ar laser to cool down** | * **Wait 5 min. for Ar laser to cool down** | ||
| * Turn off the ''MAIN SWITCH'' | * Turn off the ''MAIN SWITCH'' | ||
zeiss_lsm_880.txt · Last modified: 2024/01/28 11:47 by bioimaging
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