zeiss_lsm_880
Differences
This shows you the differences between two versions of the page.
| Both sides previous revision Previous revision Next revision | Previous revision Next revision Both sides next revision | ||
|
zeiss_lsm_880 [2017/11/23 23:46] bioimaging [Microscope overview] |
zeiss_lsm_880 [2018/10/19 19:58] bioimaging |
||
|---|---|---|---|
| Line 29: | Line 29: | ||
| ===== Microscope overview ===== | ===== Microscope overview ===== | ||
| - | {{ :airyscan.jpg?0x380}} The Zeiss LSM 880 with Airyscan is a //confocal point-scanning microscope// able to generate high-resolution three-dimensional images of thick specimens with high sensitivity and low photodamage. It is an inverted microscope specially suitable for live cell imaging and photobleaching experiments, equipped with a large size incubator for temperature control and CO2 supply and with Definite Focus for hardware focus control during long time-lapse acquisitions. Its [[http://www.zeiss.com/content/dam/Microscopy/Products/laser-scan/LSM%20880/zeiss-lsm880-airyscan-principle-672.jpg|Airyscan]] 32 channel area detector is able to collect all light from an Airy pattern simultaneously, allowing for increased resolution (140 nm laterally and 400 nm axially, at 488 nm). Its scanning unit further includes two PMT detectors (one of them cooled) as well as a GaAsP detector for increased sensitivity (45% QE compared to ~25% QE for conventional PMT). It is equipped with lasers from violet to far red (405, 458, 488, 514, 561, 594 and 633 nm excitation wavelengths). With this system you can perform optical sectioning of fluorescent samples which are too thick for a widefield system such as the [[zeiss_cell_observer|Zeiss Cell Observer]] or the [[zeiss_axiovert_200m|Zeiss Axiovert 200M]]. Image resolution with Airyscan is higher than the [[zeiss_lsm_710|Zeiss LSM 710]] and detection sensitivity is also higher than faster confocal systems such as the spinning disks [[3i_marianas_sdc|3i Marianas SDC]] and [[zeiss_cell_observer_sd|Zeiss Cell Observer SD]]. If your personal computer cannot handle all the data you collected, check out the [[big_guy|Big Guy]] or [[colossus|Colossus]]. | + | {{ :airyscan.jpg?0x380}} The Zeiss LSM 880 with Airyscan is a //confocal point-scanning microscope// able to generate high-resolution three-dimensional images of thick specimens with high sensitivity and low photodamage. It is an inverted microscope especially suitable for live cell imaging and photobleaching experiments, equipped with a large size incubator for temperature control and CO2 supply and with Definite Focus for hardware focus control during long time-lapse acquisitions. Its [[http://www.zeiss.com/content/dam/Microscopy/Products/laser-scan/LSM%20880/zeiss-lsm880-airyscan-principle-672.jpg|Airyscan]] 32 channel area detector is able to collect all light from an Airy pattern simultaneously, allowing for increased resolution (140 nm laterally and 400 nm axially, at 488 nm). Its scanning unit further includes two PMT detectors (one of them cooled) as well as a GaAsP detector for increased sensitivity (45% QE compared to ~25% QE for conventional PMT). It is equipped with lasers from violet to far red (405, 458, 488, 514, 561, 594 and 633 nm excitation wavelengths). With this system you can perform optical sectioning of fluorescent samples which are too thick for a widefield system such as the [[zeiss_cell_observer|Zeiss Cell Observer]] or the [[zeiss_axiovert_200m|Zeiss Axiovert 200M]]. Image resolution with Airyscan is higher than the [[zeiss_lsm_710|Zeiss LSM 710]] and detection sensitivity is also higher than faster confocal systems such as the spinning disks [[3i_marianas_sdc|3i Marianas SDC]] and [[zeiss_cell_observer_sd|Zeiss Cell Observer SD]]. If your personal computer cannot handle all the data you collected, check out the [[big_guy|Big Guy]] or [[colossus|Colossus]]. |
| {{info.gif?|}} Click on the image on the left to see the system beam path in higher detail | {{info.gif?|}} Click on the image on the left to see the system beam path in higher detail | ||
| Line 36: | Line 36: | ||
| ===== Booking Rules ===== | ===== Booking Rules ===== | ||
| - | The Zeiss LSM 710 booking is divided in three daily slots: | + | The Zeiss LSM 880 booking is divided in three daily slots: |
| | 9:00 to 13:00 | 13:00 to 17:00 | 17:00 to 21:00 | | | 9:00 to 13:00 | 13:00 to 17:00 | 17:00 to 21:00 | | ||
| Line 82: | Line 82: | ||
| {{zeiss_lsm_880_1.jpg?100}} | {{zeiss_lsm_880_1.jpg?100}} | ||
| * Turn the computer on | * Turn the computer on | ||
| - | * Login with your unit account | + | * Login with lsm 880 user account |
| * Wait for network icon to change to ''connected'' state | * Wait for network icon to change to ''connected'' state | ||
| * Start the **ZEN** software | * Start the **ZEN** software | ||
zeiss_lsm_880.txt ยท Last modified: 2024/01/28 11:47 by bioimaging
Page Tools
