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zeiss_lsm_880 [2024/01/28 11:46]
bioimaging
zeiss_lsm_880 [2024/01/28 11:47] (current)
bioimaging [Microscope overview]
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 ===== Microscope overview ===== ===== Microscope overview =====
  
-{{  :​confocal_pinhole.png?​360}} The Zeiss LSM 880 with Airyscan is a [[https://​www.youtube.com/​watch?​v=rTkjQ6Q0p5I|point-scanning confocal microscope]] able to generate high-resolution three-dimensional images of thick specimens with high sensitivity,​ high speed and low photodamage. It is an inverted microscope especially suitable for live cell imaging and photobleaching experiments,​ equipped with a large cage incubator and a stage top incubator for temperature control and CO2 supply and with Definite Focus for hardware focus control during long time-lapse acquisitions. The stage is motorized and furthermore equipped with a piezo for Z displacement so fast 4D imaging is possible in multiple stage positions. Its [[http://​www.zeiss.com/​content/​dam/​Microscopy/​Products/​laser-scan/​LSM%20880/​zeiss-lsm880-airyscan-principle-672.jpg|Airyscan]] with 32 concentrically arranged GaAsP detection elements is able to collect all light from an Airy pattern simultaneously,​ allowing for increased resolution (140 nm laterally and 400 nm axially, at 488 nm). It can also be used in [[https://​www.youtube.com/​watch?​v=ln6VUmLzLLc|Fast Mode]], which enables parallel excitation and detection of four image pixels. The result is a speed improvement by a factor 4x, while maintaining the sensitivity of Airyscan and 1.5x resolution improvement. Its scanning unit further includes two PMT detectors (one of them cooled) as well as a GaAsP detector for increased sensitivity (45% QE compared to ~25% QE for conventional PMT). It is equipped with lasers from violet to far red (405, 458, 488, 514, 561, 594 and 633 nm excitation wavelengths).+{{  :​confocal_pinhole.png?​340}} The Zeiss LSM 880 with Airyscan is a [[https://​www.youtube.com/​watch?​v=rTkjQ6Q0p5I|point-scanning confocal microscope]] able to generate high-resolution three-dimensional images of thick specimens with high sensitivity,​ high speed and low photodamage. It is an inverted microscope especially suitable for live cell imaging and photobleaching experiments,​ equipped with a large cage incubator and a stage top incubator for temperature control and CO2 supply and with Definite Focus for hardware focus control during long time-lapse acquisitions. The stage is motorized and furthermore equipped with a piezo for Z displacement so fast 4D imaging is possible in multiple stage positions. Its [[http://​www.zeiss.com/​content/​dam/​Microscopy/​Products/​laser-scan/​LSM%20880/​zeiss-lsm880-airyscan-principle-672.jpg|Airyscan]] with 32 concentrically arranged GaAsP detection elements is able to collect all light from an Airy pattern simultaneously,​ allowing for increased resolution (140 nm laterally and 400 nm axially, at 488 nm). It can also be used in [[https://​www.youtube.com/​watch?​v=ln6VUmLzLLc|Fast Mode]], which enables parallel excitation and detection of four image pixels. The result is a speed improvement by a factor 4x, while maintaining the sensitivity of Airyscan and 1.5x resolution improvement. Its scanning unit further includes two PMT detectors (one of them cooled) as well as a GaAsP detector for increased sensitivity (45% QE compared to ~25% QE for conventional PMT). It is equipped with lasers from violet to far red (405, 458, 488, 514, 561, 594 and 633 nm excitation wavelengths).
  
-  * [[https://​www.youtube.com/​watch?​v=sGTNBXq8omg|ZEISS Webinar: LSM 880 with Airyscan - Revolutionize Your Confocal Imaging ​(video)]] +  * [[https://​www.youtube.com/​watch?​v=sGTNBXq8omg|ZEISS Webinar: LSM 880 with Airyscan - Revolutionize Your Confocal Imaging]] 
-  * [[https://​www.youtube.com/​watch?​v=VuxgaKDa_DU|ZEISS Webinar: Fast Mode for ZEISS LSM 880 with Airyscan ​(video)]]+  * [[https://​www.youtube.com/​watch?​v=VuxgaKDa_DU|ZEISS Webinar: Fast Mode for ZEISS LSM 880 with Airyscan]]
  
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zeiss_lsm_880.txt · Last modified: 2024/01/28 11:47 by bioimaging