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zeiss_lsm_880

Location: Room P2-A-22 ( 47217)
Manufacturer: ZEISS Microscopy
Model: LSM 880 with Airyscan (click for Zeiss webinar)
Nickname: "880"
Software: ZEN 2.3 SP1 (black edition)
Year: 2016
SN: 2811000267
 
 
 
 
Zeiss LSM 880 Quality Control
Zeiss LSM 880 Usage Statistics

Microscope overview

The Zeiss LSM 880 with Airyscan is a point-scanning confocal microscope able to generate high-resolution three-dimensional images of thick specimens with high sensitivity, high speed and low photodamage. It is an inverted microscope especially suitable for live cell imaging and photobleaching experiments, equipped with a large cage incubator and a stage top incubator for temperature control and CO2 supply and with Definite Focus for hardware focus control during long time-lapse acquisitions. The stage is motorized and furthermore equipped with a piezo for Z displacement so fast 4D imaging is possible in multiple stage positions. Its Airyscan with 32 concentrically arranged GaAsP detection elements is able to collect all light from an Airy pattern simultaneously, allowing for increased resolution (140 nm laterally and 400 nm axially, at 488 nm). It can also be used in Fast Mode, which enables parallel excitation and detection of four image pixels. The result is a speed improvement by a factor 4x, while maintaining the sensitivity of Airyscan and 1.5x resolution improvement. Its scanning unit further includes two PMT detectors (one of them cooled) as well as a GaAsP detector for increased sensitivity (45% QE compared to ~25% QE for conventional PMT). It is equipped with lasers from violet to far red (405, 458, 488, 514, 561, 594 and 633 nm excitation wavelengths). With this system you can perform optical sectioning of fluorescent samples which are too thick for a widefield system such as the Zeiss Cell Observer. Image resolution with Airyscan is higher than the Zeiss LSM 710 and detection sensitivity is also higher than the spinning disks 3i Marianas SDC and Zeiss Cell Observer SD. If your personal computer cannot handle all the data you collected, check out the Colossus.

Data files older than 1 month will be automatically deleted on this system, please copy your data to the iMM server using the desktop link.

Additional information

Booking Rules

  1. Users can book at maximum 8 hours per week
  2. This usage restriction does not apply for weekends and for working days before 9:00 and after 21:00
  3. Exceptions to these rules require approval from José Rino.

System components

LASERs

Laser Unit Wavelength Maximum Power
Diode 405-30 405 nm 30 mW
Argon 458, 488 and 514 nm 25 mW
DPSS 561-20 561 nm 20 mW
HeNe594 594 nm 2 mW
HeNe633 633 nm 5 mW

Objectives

Magnification Model Immersion NA WD (mm) Reference
10x EC Plan-Neofluar Air 0.30 5.20 420340-9901-000
20x Plan-Apochromat Air 0.80 0.55 420650-9901-000
40x C-Apochromat Corr Water 1.20 0.28 421767-9970-000
40x LD C-Apochromat Corr Water 1.10 0.62 421867-9970-000
63x Plan-Apochromat DIC Oil 1.40 0.19 420782-9900-799

Upon request:

Magnification Model Immersion NA WD (mm) Reference
25x LCI Plan-Neofluar Corr DIC Oil/Glyc/W 0.80 0.21 420852-9972-000
40x Plan-Apochromat Corr Dry 0.95 0.25 420660-9970-000

Filtersets (Ocular)

Position Filterset Reference Excitation Dichroic Emission
2 Cyan 49001 426-446 nm 455 nm 460-500 nm
3 Green FS38HE 450-490 nm 495 nm 500-550 nm
4 Red FS43 533-558 nm 570 nm 570-640 nm
5 Blue FS49 G 365 nm 395 nm 420-470 nm

Microscope Turn On Procedure

  • Turn on the MAIN SWITCH

zeiss_lsm_880_1.jpg

  • Turn on the SYSTEMS/PC switch
  • Turn on the computer
  • Login in to Windows with your Agendo credentials
  • Turn on the COMPONENTS switch
  • Start the ZEN Black software

Microscope Turn Off Procedure

If there is another user for this microscope in the next hour:

  • Exit the ZEN software, leave the lasers on
  • Log off the computer
  • Clean up immersion objectives

Else:

  • Turn off the lasers on ZEN
  • Clean up immersion objectives
  • Exit the ZEN software
  • Shutdown the computer
  • Turn off the two switches SYSTEMS/PC and COMPONENTS
  • Wait 5 min. for Ar laser to cool down
  • Turn off the MAIN SWITCH

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zeiss_lsm_880.txt · Last modified: 2022/09/05 18:32 by bioimaging