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zeiss_lsm_710 [2022/01/20 13:34]
bioimaging [Microscope Turn On Procedure]
zeiss_lsm_710 [2024/01/18 01:18] (current)
bioimaging
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-<a href="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=zeiss_lsm_710_wiki_new.png"><​img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=zeiss_lsm_710_wiki_new.png" width=300></​a>​+<a href="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=zeiss_lsm_710_wiki_2022.png"><​img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=zeiss_lsm_710_wiki_2022.png" width=300></​a>​
 <td style="​border:​0px solid white;">​ <p style="​line-height:​1.8">​ <td style="​border:​0px solid white;">​ <p style="​line-height:​1.8">​
-<​b>​Location</​b>: ​<a href="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=zeiss_lsm_710_map_new_p2a24.png">​Room P2-A-24</​a>​ (<img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=phone_neg.png"​ width=15>​ 47219) ​<br>  +<​b>​Location</​b>: ​no longer available ​<br>  
-<​b>​Manufacturer</​b>:​ <a href="​http://​www.zeiss.de/​micro"​ target="​_blank">​Carl Zeiss MicroImaging</​a><​br>​+<​b>​Manufacturer</​b>:​ <a href="​http://​www.zeiss.de/​micro"​ target="​_blank">​ZEISS Microscopy</​a><​br>​
 <​b>​Model</​b>:​ LSM 710<​br>​ <​b>​Model</​b>:​ LSM 710<​br>​
 <​b>​Nickname</​b>:​ "​710"<​br>​ <​b>​Nickname</​b>:​ "​710"<​br>​
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 <​b>​Year</​b>:​ 2010 <br> <​b>​Year</​b>:​ 2010 <br>
 <​b>​SN</​b>:​ 2503000400 <br> <​b>​SN</​b>:​ 2503000400 <br>
 +&nbsp <br>
 +Data will be deleted after: <b>3 months</​b>​
 &nbsp <br> &nbsp <br>
 &nbsp <br> &nbsp <br>
 &nbsp <br> &nbsp <br>
-&rarr; <a href="​https://​my.agendo.science/​calendar/?​id=MjRjYWxlbmRhcnM="><​img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=date.png"> ​ Zeiss LSM 710 Booking</​a>​ 
- <​br> ​ 
 &rarr; <a href="/​facility/​bioimaging/​doku.php?​id=zeiss_lsm_710_quality"><​img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=list-icon.png"​ width=18>​ Zeiss LSM 710 Quality Control</​a>​ &rarr; <a href="/​facility/​bioimaging/​doku.php?​id=zeiss_lsm_710_quality"><​img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=list-icon.png"​ width=18>​ Zeiss LSM 710 Quality Control</​a>​
  <​br>​  <​br>​
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 ===== Microscope overview ===== ===== Microscope overview =====
-{{  :lsm710_beampath.png?410|}} The Zeiss LSM 710 is a [[https://imm.medicina.ulisboa.pt/facility/​bioimaging/​lib/​exe/​fetch.php?media=confocal.png|confocal point-scanning microscope]] able to generate high-resolution three-dimensional images of thick specimens with high sensitivity and low photodamage. It is an inverted microscope ​specially ​suitable for live cell imaging and photobleaching experiments,​ equipped with a large cage incubator and a stage top incubator for temperature control and CO2 supply. Its scanning unit has a spectral recycling loop, two sensitive PMT detectors and a filter-free spectral separation module that can be continuously set over the entire wavelength range (i.e. you can specify which wavelength range you want to detect). It is equipped with lasers from violet to far red (405, 458, 488, 514, 561 and 633 nm excitation wavelengths). With this system you can perform optical sectioning of fluorescent samples which are too thick for a widefield system such as the [[zeiss_cell_observer|Zeiss Cell Observer]] or the [[zeiss_axiovert_200m|Zeiss Axiovert 200M]]. Image resolution and detection sensitivity are higher than faster confocal systems such as the spinning disks [[3i_marianas_sdc|3i Marianas SDC]] and [[zeiss_cell_observer_sd|Zeiss Cell Observer SD]], but image acquisition is slower. If you need to use a 594 nm laser or increased resolution and sensitivity,​ or are performing long time-lapse experiments in live samples (aqueous media) and need hardware focus control, check the [[zeiss_lsm_880|Zeiss LSM 880]] confocal point-scanning ​system ​with Airyscan and Definite Focus. If your personal computer cannot handle all the data you collected, check out the [[big_guy|Big Guy]] or [[colossus|Colossus]].+{{  :confocal_pinhole.png?350|}} The Zeiss LSM 710 is a [[https://www.youtube.com/watch?v=rTkjQ6Q0p5I|confocal point-scanning microscope]] able to generate high-resolution three-dimensional images of thick specimens with high sensitivity and low photodamage. It is an inverted microscope ​especially ​suitable for live cell imaging and photobleaching experiments,​ equipped with a large cage incubator and a stage top incubator for temperature control and CO2 supply. The stage is motorized so imaging is possible in multiple stage positions or tiled acquisitions. Its scanning unit has a spectral recycling loop, two sensitive PMT detectors and a filter-free spectral separation module that can be continuously set over the entire wavelength range (i.e. you can specify which wavelength range you want to detect). It is equipped with lasers from violet to far red (405, 458, 488, 514, 561 and 633 nm excitation wavelengths). With this system you can perform optical sectioning of fluorescent samples which are too thick for a widefield system such as the [[zeiss_cell_observer|Zeiss Cell Observer]] or the [[zeiss_axiovert_200m|Zeiss Axiovert 200M]]. Image resolution and detection sensitivity are higher than faster confocal systems such as the spinning disks [[3i_marianas_sdc|3i Marianas SDC]] and [[zeiss_cell_observer_sd|Zeiss Cell Observer SD]], but image acquisition is slower. If you need to use a 594 nm laser or increased resolution and sensitivity,​ or are performing long time-lapse experiments in live samples (aqueous media) and need hardware focus control, check the [[zeiss_lsm_880|Zeiss LSM 880]] or [[zeiss_lsm_980|Zeiss LSM 980]] confocal point-scanning ​systems ​with Airyscan and Definite Focus. If your personal computer cannot handle all the data you collected, check out the [[colossus|Colossus]].
  
 {{:​warning.png?​25|}} Data files older than **3 months** will be automatically deleted on this system, please copy your data to the iMM server using the desktop link. {{:​warning.png?​25|}} Data files older than **3 months** will be automatically deleted on this system, please copy your data to the iMM server using the desktop link.
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 ==== LASERs ==== ==== LASERs ====
-^  Laser Unit  ^  Wavelength ​ ^  Maximum Power  ​^ ​ Current Status ​ ^ +^  Laser Unit  ^  Wavelength ​ ^  Maximum Power  ^ 
-|  Diode 405-30 ​ |  405 nm  |  30 mW  ​| ​ ok  | +|  Diode 405-30 ​ |  405 nm  |  30 mW  | 
-|  Argon  |  458, 488 and 514 nm  |  25 mW  ​| ​ ok  | +|  Argon  |  458, 488 and 514 nm  |  25 mW  | 
-|  DPSS 561-10 ​ |  561 nm  |  15 mW  ​| ​ ok  | +|  DPSS 561-10 ​ |  561 nm  |  15 mW  | 
-|  HeNe633 ​ |  633 nm  |  5 mW  ​| ​ ok  |+|  HeNe633 ​ |  633 nm  |  5 mW  |
  
 ==== Objectives ==== ==== Objectives ====
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   * If you need CO2, open the CO2 valve   * If you need CO2, open the CO2 valve
   * Turn on the computer   * Turn on the computer
-  * Log in to Windows ​(Bioimaging User)+  * Login in to Windows ​with your Agendo credentials
   * Turn on the ''​COMPONENTS''​ switch   * Turn on the ''​COMPONENTS''​ switch
 {{switches_710.jpg?​100}} {{switches_710.jpg?​100}}
zeiss_lsm_710.1642682051.txt.gz · Last modified: 2022/01/20 13:34 by bioimaging