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zeiss_lsm_710 [2020/02/26 22:29]
bioimaging
zeiss_lsm_710 [2024/01/18 01:18] (current)
bioimaging
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-<a href="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=zeiss_lsm_710_wiki_new.png"><​img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=zeiss_lsm_710_wiki_new.png" width=300></​a>​+<a href="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=zeiss_lsm_710_wiki_2022.png"><​img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=zeiss_lsm_710_wiki_2022.png" width=300></​a>​
 <td style="​border:​0px solid white;">​ <p style="​line-height:​1.8">​ <td style="​border:​0px solid white;">​ <p style="​line-height:​1.8">​
-<​b>​Location</​b>: ​<a href="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=zeiss_lsm_710_map_new.png">​Room P3-A-10</​a>​ (<img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=phone_neg.png"​ width=15>​ 47331) ​<br>  +<​b>​Location</​b>: ​no longer available ​<br>  
-<​b>​Manufacturer</​b>:​ <a href="​http://​www.zeiss.de/​micro"​ target="​_blank">​Carl Zeiss MicroImaging</​a><​br>​+<​b>​Manufacturer</​b>:​ <a href="​http://​www.zeiss.de/​micro"​ target="​_blank">​ZEISS Microscopy</​a><​br>​
 <​b>​Model</​b>:​ LSM 710<​br>​ <​b>​Model</​b>:​ LSM 710<​br>​
 <​b>​Nickname</​b>:​ "​710"<​br>​ <​b>​Nickname</​b>:​ "​710"<​br>​
-<​b>​Software</​b>:​ ZEN 2012<​br>​+<​b>​Software</​b>:​ ZEN 2012 SP5 FP3 (black)<br>
 <​b>​Year</​b>:​ 2010 <br> <​b>​Year</​b>:​ 2010 <br>
 <​b>​SN</​b>:​ 2503000400 <br> <​b>​SN</​b>:​ 2503000400 <br>
 +&nbsp <br>
 +Data will be deleted after: <b>3 months</​b>​
 &nbsp <br> &nbsp <br>
 &nbsp <br> &nbsp <br>
 &nbsp <br> &nbsp <br>
-&rarr; <a href="​https://​imm.medicina.ulisboa.pt/​intranet/​booking/​51"><​img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=date.png"> ​ Zeiss LSM 710 Booking</​a>​ 
- <​br> ​ 
 &rarr; <a href="/​facility/​bioimaging/​doku.php?​id=zeiss_lsm_710_quality"><​img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=list-icon.png"​ width=18>​ Zeiss LSM 710 Quality Control</​a>​ &rarr; <a href="/​facility/​bioimaging/​doku.php?​id=zeiss_lsm_710_quality"><​img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=list-icon.png"​ width=18>​ Zeiss LSM 710 Quality Control</​a>​
  <​br>​  <​br>​
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 ===== Microscope overview ===== ===== Microscope overview =====
-{{  :lsm710_beampath.png?410|}} The Zeiss LSM 710 is a //confocal point-scanning microscope// able to generate high-resolution three-dimensional images of thick specimens with high sensitivity and low photodamage. It is an inverted microscope ​specially ​suitable for live cell imaging and photobleaching experiments,​ equipped with a large size incubator ​for temperature control (but no stage incubator for CO2 supply). Its scanning unit has a spectral recycling loop, two sensitive PMT detectors and a filter-free spectral separation module that can be continuously set over the entire wavelength range (i.e. you can specify which wavelength range you want to detect). It is equipped with lasers from violet to far red (405, 458, 488, 514, 561 and 633 nm excitation wavelengths). With this system you can perform optical sectioning of fluorescent samples which are too thick for a widefield system such as the [[zeiss_cell_observer|Zeiss Cell Observer]] or the [[zeiss_axiovert_200m|Zeiss Axiovert 200M]]. Image resolution and detection sensitivity are higher than faster confocal systems such as the spinning disks [[3i_marianas_sdc|3i Marianas SDC]] and [[zeiss_cell_observer_sd|Zeiss Cell Observer SD]], but image acquisition is slower. If you need to use a 594 nm laser or increased resolution and sensitivity,​ or are performing long time-lapse experiments in live samples (aqueous media) and need hardware focus control, check the [[zeiss_lsm_880|Zeiss LSM 880]] confocal point-scanning ​system ​with Airyscan and Definite Focus. If your personal computer cannot handle all the data you collected, check out the [[big_guy|Big Guy]] or [[colossus|Colossus]]. +{{  :confocal_pinhole.png?350|}} The Zeiss LSM 710 is a [[https://www.youtube.com/​watch?​v=rTkjQ6Q0p5I|confocal point-scanning microscope]] able to generate high-resolution three-dimensional images of thick specimens with high sensitivity and low photodamage. It is an inverted microscope ​especially ​suitable for live cell imaging and photobleaching experiments,​ equipped with a large cage incubator ​and a stage top incubator for temperature control and CO2 supply. The stage is motorized so imaging is possible in multiple stage positions or tiled acquisitions. Its scanning unit has a spectral recycling loop, two sensitive PMT detectors and a filter-free spectral separation module that can be continuously set over the entire wavelength range (i.e. you can specify which wavelength range you want to detect). It is equipped with lasers from violet to far red (405, 458, 488, 514, 561 and 633 nm excitation wavelengths). With this system you can perform optical sectioning of fluorescent samples which are too thick for a widefield system such as the [[zeiss_cell_observer|Zeiss Cell Observer]] or the [[zeiss_axiovert_200m|Zeiss Axiovert 200M]]. Image resolution and detection sensitivity are higher than faster confocal systems such as the spinning disks [[3i_marianas_sdc|3i Marianas SDC]] and [[zeiss_cell_observer_sd|Zeiss Cell Observer SD]], but image acquisition is slower. If you need to use a 594 nm laser or increased resolution and sensitivity,​ or are performing long time-lapse experiments in live samples (aqueous media) and need hardware focus control, check the [[zeiss_lsm_880|Zeiss LSM 880]] or [[zeiss_lsm_980|Zeiss LSM 980]] confocal point-scanning ​systems ​with Airyscan and Definite Focus. If your personal computer cannot handle all the data you collected, check out the [[colossus|Colossus]].
- +
-{{:​info.png?​25|}} Click on the image on the right to see the system beam path in higher detail+
  
 {{:​warning.png?​25|}} Data files older than **3 months** will be automatically deleted on this system, please copy your data to the iMM server using the desktop link. {{:​warning.png?​25|}} Data files older than **3 months** will be automatically deleted on this system, please copy your data to the iMM server using the desktop link.
  
 ===== Booking Rules ===== ===== Booking Rules =====
-The Zeiss LSM 710 booking is divided in three daily slots: +  ​- **Users** can book at maximum **8 hours per week** 
-|  9:00 to 13:00  |  13:00 to 17:00  |  17:00 to 21:00  | +  - This usage restriction does not apply for **weekends** and for working ​days **before 9:00** and **after 21:00**
- +
-  ​**user** can book at maximum **2 sessions ​per week** +
-  - Each **session must fit inside one of the three daily slots** (i.e. a session booked from 11:00 to 14:00 counts as 2 sessions) +
-  - The above rules do not apply for **weekends** and for work days **before 9:00** and **after 21:00** +
-  - The above rules do not apply for sessions booked on the very same day or **24 hours ahead**+
   - Exceptions to these rules require **approval from [[joserino@medicina.ulisboa.pt|José Rino]]**.   - Exceptions to these rules require **approval from [[joserino@medicina.ulisboa.pt|José Rino]]**.
-  - Users must indicate which lasers they are going to use in the booking Description field +
-  - Users must indicate if they will use the incubator in the booking Description field (write down 37C INCUBATOR)+
 ===== System components ===== ===== System components =====
  
 ==== LASERs ==== ==== LASERs ====
-^  Laser Unit  ^  Wavelength ​ ^  Maximum Power  ​^ ​ Current Status ​ ^ +^  Laser Unit  ^  Wavelength ​ ^  Maximum Power  ^ 
-|  Diode 405-30 ​ |  405 nm  |  30 mW  ​| ​ ok  | +|  Diode 405-30 ​ |  405 nm  |  30 mW  | 
-|  Argon  |  458, 488 and 514 nm  |  25 mW  ​| ​ ok  | +|  Argon  |  458, 488 and 514 nm  |  25 mW  | 
-|  DPSS 561-10 ​ |  561 nm  |  15 mW  ​| ​ ok  | +|  DPSS 561-10 ​ |  561 nm  |  15 mW  | 
-|  HeNe633 ​ |  633 nm  |  5 mW  ​| ​ ok  |+|  HeNe633 ​ |  633 nm  |  5 mW  |
  
 ==== Objectives ==== ==== Objectives ====
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 |  10x  |  EC Plan-Neofluar ​ |  Air  |  0.30  |  5.2  |  [[https://​www.micro-shop.zeiss.com/​en/​de/​shop/​objectives/​420340-9901-000/​Objective-EC-Plan-Neofluar-10x-0.30-M27|420340-9901-000]] ​ | |  10x  |  EC Plan-Neofluar ​ |  Air  |  0.30  |  5.2  |  [[https://​www.micro-shop.zeiss.com/​en/​de/​shop/​objectives/​420340-9901-000/​Objective-EC-Plan-Neofluar-10x-0.30-M27|420340-9901-000]] ​ |
 |  20x  |  Plan-Apochromat ​ |  Air  |  0.80  |  0.55  |  [[https://​www.micro-shop.zeiss.com/​en/​de/​shop/​objectives/​420650-9901-000/​Objective-Plan-Apochromat-20x-0.8-M27|420650-9901-000]] ​ | |  20x  |  Plan-Apochromat ​ |  Air  |  0.80  |  0.55  |  [[https://​www.micro-shop.zeiss.com/​en/​de/​shop/​objectives/​420650-9901-000/​Objective-Plan-Apochromat-20x-0.8-M27|420650-9901-000]] ​ |
-|  40x  |  EC Plan-Neofluar DIC  |  Oil  |  1.30  |  0.20  |  [[https://​www.micro-shop.zeiss.com/​en/​de/​shop/​objectives/​440452-9903-000/​Objective-EC-Plan-Neofluar-40x-1.30-Oil-DIC|440452-9903-000]] ​ | +|  40x  |  EC Plan-Neofluar DIC  |  Oil  |  1.30  |  0.20  |  [[https://​www.micro-shop.zeiss.com/​en/​de/​shop/​objectives/​420462-9900-000/​Objective-EC-Plan-Neofluar-40x-1.30-Oil-DIC-M27|420462-9900-000]] ​ | 
-|  63x  |  Plan-Apochromat DIC  |  Oil  |  1.40  |  0.19  |  [[https://​www.micro-shop.zeiss.com/​en/​de/​shop/​objectives/​440762-9904-000/​Objective-Plan-Apochromat-63x-1.40-Oil-DIC|440762-9904-000]] ​ |+|  63x  |  Plan-Apochromat DIC  |  Oil  |  1.40  |  0.19  |  [[https://​www.micro-shop.zeiss.com/​en/​de/​shop/​objectives/​420782-9900-000/​Objective-Plan-Apochromat-63x-1.4-Oil-DIC-M27|420782-9900-000]] ​ |
  
 **Upon request:** **Upon request:**
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 ==== Filtersets (Ocular) ==== ==== Filtersets (Ocular) ====
  
-^  Position ​ ^  Filterset ​ ^  Reference ​ ^  Excitation ​ ^  Emission ​ ^ +^  Position ​ ^  Filterset ​ ^  Reference ​ ^  Excitation ​ ​^ ​ Dichroic ​ ​^ ​ Emission ​ ^ 
-|  1  |  Green  |  [[https://​www.micro-shop.zeiss.com/?​s=163176628fcf844&​l=en&​p=us&​f=f&​a=v&​b=f&​id=000000-1031-346&​o=|FS38]] ​ |  450-490 nm  |  500-550 nm  | +|  1  |  Green  |  [[https://​www.micro-shop.zeiss.com/?​s=163176628fcf844&​l=en&​p=us&​f=f&​a=v&​b=f&​id=000000-1031-346&​o=|FS38]] ​ |  450-490 ​nm  |  495 nm  |  500-550 nm  | 
-|  2  |  Red  |  [[https://​www.micro-shop.zeiss.com/?​s=163176628fcf844&​l=en&​p=us&​f=f&​a=v&​b=f&​id=000000-1114-101&​o=|FS43]] ​ |  533-558 nm  |  570-640 nm  | +|  2  |  Red  |  [[https://​www.micro-shop.zeiss.com/?​s=163176628fcf844&​l=en&​p=us&​f=f&​a=v&​b=f&​id=000000-1114-101&​o=|FS43]] ​ |  533-558 ​nm  |  570 nm  |  570-640 nm  | 
-|  3  |  Blue  |  [[https://​www.micro-shop.zeiss.com/?​s=163176628fcf844&​l=en&​p=us&​f=f&​a=v&​b=f&​id=488049-9901-000&​o=|FS49]] ​ |  G 365 nm  |  420-470 nm  |+|  3  |  Blue  |  [[https://​www.micro-shop.zeiss.com/?​s=163176628fcf844&​l=en&​p=us&​f=f&​a=v&​b=f&​id=488049-9901-000&​o=|FS49]] ​ |  G 365 nm  |  395 nm  |  420-470 nm  |
  
 ===== Microscope Turn On Procedure ===== ===== Microscope Turn On Procedure =====
   * Turn on the ''​MAIN SWITCH''​   * Turn on the ''​MAIN SWITCH''​
-  * Turn on the two switches: ​''​SYSTEMS/​PC'' ​and ''​COMPONENTS''​+  * Turn on the ''​SYSTEMS/​PC'' ​switch
 {{switches_710.jpg?​100}} {{switches_710.jpg?​100}}
   * Turn on the metal halide fluorescent light source (if needed)   * Turn on the metal halide fluorescent light source (if needed)
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   * Turn on the Ar laser using the key (if needed)   * Turn on the Ar laser using the key (if needed)
 {{laser_key_710.jpg?​100}} {{laser_key_710.jpg?​100}}
 +  * If you need CO2, open the CO2 valve
   * Turn on the computer   * Turn on the computer
-  * Log in to Windows ​(Bioimaging User) +  * Login in to Windows ​with your Agendo credentials 
-  * Login with Bioimaging User account+  * Turn on the ''​COMPONENTS''​ switch 
 +{{switches_710.jpg?​100}}
   * Check that the computer is connected to an ''​Unidentified network''​ (click on the network icon in the taskbar)   * Check that the computer is connected to an ''​Unidentified network''​ (click on the network icon in the taskbar)
 {{::​network_icon.png?​200}} {{::​network_icon.png?​200}}
   * Switch the Ar laser from ''​idle''​ ton ''​run''​ (don't change the ''​current''​)   * Switch the Ar laser from ''​idle''​ ton ''​run''​ (don't change the ''​current''​)
 {{laser_run_710.jpg?​100}} {{laser_run_710.jpg?​100}}
-  * Start the **ZEN** software+  * Start the **ZEN Black** software
  
 ===== Microscope Turn Off Procedure ===== ===== Microscope Turn Off Procedure =====
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 Else: Else:
   * Turn off the 561 nm laser and incubator in the software (if used)   * Turn off the 561 nm laser and incubator in the software (if used)
 +  * Close the LSM 710 CO2 valve (if used)
   * Switch the Ar laser from ''​run''​ to ''​idle''​ (if used)   * Switch the Ar laser from ''​run''​ to ''​idle''​ (if used)
   * Turn off the Ar laser using the key (if used)   * Turn off the Ar laser using the key (if used)
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 ---- ----
-[[resources|Back to the Resources ​page]]+[[resources|Back to the Equipment ​page]]
zeiss_lsm_710.1582752595.txt.gz · Last modified: 2020/02/26 22:29 by bioimaging