zeiss_lsm_710
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zeiss_lsm_710 [2020/06/01 19:22] bioimaging |
zeiss_lsm_710 [2020/08/05 19:03] bioimaging |
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| ===== Microscope overview ===== | ===== Microscope overview ===== | ||
| - | {{ :lsm710_beampath.png?410|}} The Zeiss LSM 710 is a //confocal point-scanning microscope// able to generate high-resolution three-dimensional images of thick specimens with high sensitivity and low photodamage. It is an inverted microscope specially suitable for live cell imaging and photobleaching experiments, equipped with a large size incubator for temperature control (but no stage incubator for CO2 supply). Its scanning unit has a spectral recycling loop, two sensitive PMT detectors and a filter-free spectral separation module that can be continuously set over the entire wavelength range (i.e. you can specify which wavelength range you want to detect). It is equipped with lasers from violet to far red (405, 458, 488, 514, 561 and 633 nm excitation wavelengths). With this system you can perform optical sectioning of fluorescent samples which are too thick for a widefield system such as the [[zeiss_cell_observer|Zeiss Cell Observer]] or the [[zeiss_axiovert_200m|Zeiss Axiovert 200M]]. Image resolution and detection sensitivity are higher than faster confocal systems such as the spinning disks [[3i_marianas_sdc|3i Marianas SDC]] and [[zeiss_cell_observer_sd|Zeiss Cell Observer SD]], but image acquisition is slower. If you need to use a 594 nm laser or increased resolution and sensitivity, or are performing long time-lapse experiments in live samples (aqueous media) and need hardware focus control, check the [[zeiss_lsm_880|Zeiss LSM 880]] confocal point-scanning system with Airyscan and Definite Focus. If your personal computer cannot handle all the data you collected, check out the [[big_guy|Big Guy]] or [[colossus|Colossus]]. | + | {{ :lsm710_beampath.png?410|}} The Zeiss LSM 710 is a [[https://imm.medicina.ulisboa.pt/facility/bioimaging/lib/exe/fetch.php?media=confocal.png|confocal point-scanning microscope]] able to generate high-resolution three-dimensional images of thick specimens with high sensitivity and low photodamage. It is an inverted microscope specially suitable for live cell imaging and photobleaching experiments, equipped with a large size incubator for temperature control (but no stage incubator for CO2 supply). Its scanning unit has a spectral recycling loop, two sensitive PMT detectors and a filter-free spectral separation module that can be continuously set over the entire wavelength range (i.e. you can specify which wavelength range you want to detect). It is equipped with lasers from violet to far red (405, 458, 488, 514, 561 and 633 nm excitation wavelengths). With this system you can perform optical sectioning of fluorescent samples which are too thick for a widefield system such as the [[zeiss_cell_observer|Zeiss Cell Observer]] or the [[zeiss_axiovert_200m|Zeiss Axiovert 200M]]. Image resolution and detection sensitivity are higher than faster confocal systems such as the spinning disks [[3i_marianas_sdc|3i Marianas SDC]] and [[zeiss_cell_observer_sd|Zeiss Cell Observer SD]], but image acquisition is slower. If you need to use a 594 nm laser or increased resolution and sensitivity, or are performing long time-lapse experiments in live samples (aqueous media) and need hardware focus control, check the [[zeiss_lsm_880|Zeiss LSM 880]] confocal point-scanning system with Airyscan and Definite Focus. If your personal computer cannot handle all the data you collected, check out the [[big_guy|Big Guy]] or [[colossus|Colossus]]. |
| {{:warning.png?25|}} Data files older than **3 months** will be automatically deleted on this system, please copy your data to the iMM server using the desktop link. | {{:warning.png?25|}} Data files older than **3 months** will be automatically deleted on this system, please copy your data to the iMM server using the desktop link. | ||
| ===== Booking Rules ===== | ===== Booking Rules ===== | ||
| - | The Zeiss LSM 710 booking is divided in three daily slots: | + | - **Users** can book at maximum **8 hours per week** |
| - | | 9:00 to 13:00 | 13:00 to 17:00 | 17:00 to 21:00 | | + | - This usage restriction does not apply for **weekends** and for working days **before 9:00** and **after 21:00** |
| - | + | ||
| - | - A **user** can book at maximum **2 sessions per week** | + | |
| - | - Each **session must fit inside one of the three daily slots** (i.e. a session booked from 11:00 to 14:00 counts as 2 sessions) | + | |
| - | - The above rules do not apply for **weekends** and for work days **before 9:00** and **after 21:00** | + | |
| - | - The above rules do not apply for sessions booked on the very same day or **24 hours ahead** | + | |
| - Exceptions to these rules require **approval from [[joserino@medicina.ulisboa.pt|José Rino]]**. | - Exceptions to these rules require **approval from [[joserino@medicina.ulisboa.pt|José Rino]]**. | ||
| - | - Users must indicate which lasers they are going to use in the booking Description field | + | |
| - | - Users must indicate if they will use the incubator in the booking Description field (write down 37C INCUBATOR) | + | |
| ===== System components ===== | ===== System components ===== | ||
zeiss_lsm_710.txt · Last modified: 2024/01/18 01:18 by bioimaging
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