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Zeiss LSM 510 (no longer available)

Location: Room P3-A-10 ( 47311)
Manufacturer: Zeiss Model: LSM 510 Year: 1998 SN: 450240

Microscope overview

The LSM 510 system is a point scanning laser confocal microscope prepared for live imaging and photobleaching experiments. The system can be equipped with a small size incubator for temperature control.

LASER units

Laser Unit Wavelength Maximum Power Current Status
Argon 458, 488 and 514 nm 25 mW not working
HeNe1 543 nm 1 mW not working
HeNe2 633 nm 5 mW ok

Objectives

Magnification Class Immersion NA More info
10x Plan-Neofluar No 0.30 440330-0000
63x Plan-Apochromat Oil 1.40 440761-9905
100x Plan-Neofluar Oil 1.30 440480-9903

Filter and Dichroic sets

Main configuration window Emission filters for channel 1 Emission filters for channel 2 Channel separation dichroic Laser separation dichroic

Microscope Turn On Procedures

  • Turn on the two UPS near the microscope.

zeiss_lsm510_ups.jpg

  • Turn the remote control on.

zeiss_lsm510_remote_control.jpg

  • Turn the HBO lamp on.

zeiss_lsm510_fluorescence.jpg

  • Turn the halogen transmitted light power source on.

zeiss_lsm510_transmited.jpg

  • Turn the computer on.
  • Login to the computer, start the LSM software.

Microscope Turn Off procedures

If there's another user for this microscope in the next two hours:

  • Close the software, leave the lasers on an log off the computer.
  • Clean up any obejctive were you used immersion oil.
  • Make sure that there realy is another user going to use the microscope.

Else:

  • Clean up any objective were you used immersion oil.
  • Turn off the lasers in the software (wait for laser cooling!)
  • Clean up immersion objectives
  • Turn off the HBO lamp.
  • Turn off the transmitted light lamp.
  • Shutdown the computer.
  • Turn off the remote control.
  • Turn off the two ups.
  • This PDF shows how to work with the system, it's not exactly the same system configuration but teaches a lot about the software.

TOP 5 Users

2008 2007
Marco Antunes (UMO) 123.5h Jennifer Geiger (UMO) 339.5h
Anna Zaidman (UMO) 90.5h Isabel Campos (UMO) 193h
Jennifer Geiger (UMO) 65h Margarida Silva (UBD) 130h
Ana Santos (UMO) 64.5h Marco Antunes (UMO) 115h
Mariana Simões (UMO) 64h Ana Santos (UMO) 99h

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zeiss_lsm_510.1460848765.txt.gz · Last modified: 2016/04/17 01:19 by bioimaging