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====== Zeiss LSM 510 (no longer available) ======
{{510_whole.jpg?0x220|}} {{510_stand.jpg?0x220|}}

<html>
<UL>
<a href="http://www.imm.fm.ul.pt/booking/old/month.php?user=ZEISSLSM51&rid=2"><img src="/facility/bioimaging/lib/exe/fetch.php?media=date.png">  Zeiss LSM 510 Booking</a>
</UL>
<UL>
<a href="/facility/bioimaging/doku.php?id=lsm_510_usage"><img src="/facility/bioimaging/lib/exe/fetch.php?media=chart_line.png">  Zeiss LSM 510 Usage Report</a>
</UL>
</html>

**Location**: no longer available
**Manufacturer**: Zeiss <html><BR></html>
**Model**: LSM 510 <html><BR></html>
**Year**: 1998 <html><BR></html>
**SN**: 450240 <html><BR></html>

===== Microscope overview =====
The LSM 510 system is a point scanning laser confocal microscope prepared for live imaging and photobleaching experiments. The system can be equipped with a small size incubator for temperature control.

==== LASER units ====
^  Laser Unit  ^  Wavelength  ^  Maximum Power  ^  Current Status  ^
|  Argon  |  458, 488 and 514 nm  |  25 mW  |  **not working**  |
|  HeNe1  |  543 nm  |  1 mW  |  **not working**  |
|  HeNe2  |  633 nm  |  5 mW  |  ok  |

==== Objectives ====
^  Magnification   ^  Class  ^  Immersion  ^  NA  ^  More info  ^
|  10x  |  Plan-Neofluar  |  No  |  0.30  |  [[https://www.micro-shop.zeiss.com/us/us_en/objektive.php?cp_sid=&f=od&p[]=440330-0000-000|440330-0000]]  |
|  63x  |  Plan-Apochromat  |  Oil  |  1.40  |  [[https://www.micro-shop.zeiss.com/us/us_en/objektive.php?cp_sid=&f=od&p[]=440761-9905-000|440761-9905]]  |
|  100x  |  Plan-Neofluar  |  Oil  |  1.30  |  [[https://www.micro-shop.zeiss.com/us/us_en/objektive.php?cp_sid=&f=od&p[]=440480-9903-000|440480-9903]]  |

==== Filter and Dichroic sets ====
|  {{510_main.jpg?120|}}  |  {{510_emmission1.jpg?120|}}  |  {{510_emmission2.jpg?120|}}  |  {{510_2dichroic.jpg?120|}}  |  {{510_1dichroic.jpg?120|}}  |
|  Main configuration window  |  Emission filters for channel 1  |  Emission filters for channel 2  |  Channel separation dichroic  |  Laser separation dichroic  |
 

===== Microscope Turn On Procedures =====
  * Turn on the two UPS near the microscope.
{{zeiss_lsm510_ups.jpg?100}}
  * Turn the remote control on.
{{zeiss_lsm510_remote_control.jpg?100}}
  * Turn the HBO lamp on.
{{zeiss_lsm510_fluorescence.jpg?100}}
  * Turn the halogen transmitted light power source on.
{{zeiss_lsm510_transmited.jpg?100}}
  * Turn the computer on.
  * Login to the computer, start the LSM software.

===== Microscope Turn Off procedures =====
If there's another user for this microscope in the next two hours:
  * Close the software, leave the lasers on an log off the computer. 
  * Clean up any objective were you used immersion oil.
  * Make sure that there really is another user going to use the microscope.
Else:
  * Clean up any objective were you used immersion oil.
  * Turn off the lasers in the software (wait for laser cooling!)
  * Clean up immersion objectives
  * Turn off the HBO lamp.
  * Turn off the transmitted light lamp.
  * Shutdown the computer.
  * Turn off the remote control.
  * Turn off the two ups.



===== External links =====
  * This {{lsm510.pdf|PDF}} shows how to work with the system, it's not exactly the same system configuration but teaches a lot about the software.

----
[[resources|Back to the Equipment page]]
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