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zeiss_lightsheet_z1 [2019/10/08 16:11]
bioimaging
zeiss_lightsheet_z1 [2020/06/01 19:24] (current)
bioimaging
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 <​html>​ <​html>​
-<img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=zeiss_lightsheet_z1_name.jpg">+<img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=zeiss_lightsheet_z1_name_new.png">
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-<a href="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=zeiss_lightsheet_z1_wiki.jpg"><​img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=zeiss_lightsheet_z1_wiki.jpg" width=300></​a>​+<a href="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=zeiss_lightsheet_z1_wiki_new.png"><​img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=zeiss_lightsheet_z1_wiki_new.png" width=300></​a>​
 <td style="​border:​0px solid white;">​ <p style="​line-height:​1.8">​ <td style="​border:​0px solid white;">​ <p style="​line-height:​1.8">​
-<​b>​Location</​b>:​ <a href="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=zeiss_lightsheet_z1_map.jpg">​Room P3-A-5</​a><​br> ​+<​b>​Location</​b>:​ <a href="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=zeiss_lightsheet_z1_map_new.png">​Room P3-A-5</​a><​br> ​
 <​b>​Manufacturer</​b>:​ <a href="​http://​www.zeiss.de/​micro"​ target="​_blank">​Carl Zeiss MicroImaging</​a><​br>​ <​b>​Manufacturer</​b>:​ <a href="​http://​www.zeiss.de/​micro"​ target="​_blank">​Carl Zeiss MicroImaging</​a><​br>​
 <​b>​Model</​b>:​ <a href="​http://​www.zeiss.com/​microscopy/​en_de/​products/​imaging-systems/​lightsheet-z-1.html"​ target="​_blank">​Lightsheet Z.1</​a><​br>​ <​b>​Model</​b>:​ <a href="​http://​www.zeiss.com/​microscopy/​en_de/​products/​imaging-systems/​lightsheet-z-1.html"​ target="​_blank">​Lightsheet Z.1</​a><​br>​
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 &nbsp <br> &nbsp <br>
 &nbsp <br> &nbsp <br>
-&rarr; <a href="​https://​imm.medicina.ulisboa.pt/​intranet/booking/129"><​img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=date.png"> ​ Zeiss Lightsheet Z1 Booking</​a>​+&rarr; <a href="​https://​my.agendo.science/calendar/?​id=MjljYWxlbmRhcnM="><​img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=date.png"> ​ Zeiss Lightsheet Z1 Booking</​a>​
  <​br> ​  <​br> ​
 &rarr; <a href="/​facility/​bioimaging/​doku.php?​id=zeiss_lightsheet_z1_usage"><​img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=chart_line.png"> ​ Zeiss Lightsheet Z1 Usage Statistics</​a>​ &rarr; <a href="/​facility/​bioimaging/​doku.php?​id=zeiss_lightsheet_z1_usage"><​img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=chart_line.png"> ​ Zeiss Lightsheet Z1 Usage Statistics</​a>​
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 [[https://​imm.medicina.ulisboa.pt/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=lightsheet_illumination.jpg|Since only a single thin section of the sample is illuminated by the light sheet]], optical sectioning is achieved without a pinhole or image processing deconvolution. Light from the in-focus plane is collected by a sCMOS camera rather than pixel by pixel as in a point scanning laser confocal. This allows you to collect images faster and with less excitation light than you would with many other optical-sectioning microscopy techniques. You can image living samples in water or fixed samples where [[http://​blogs.zeiss.com/​microscopy/​news/​en/​references-for-clearing-protocols/​|tissue clearing]] has been performed. Samples are mounted not on coverslips but inside [[https://​imm.medicina.ulisboa.pt/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=lightsheet_chambers.jpg|special chambers]] that provide heating and cooling. The system is equipped with lasers from green to far red (488, 561 and 638 nm excitation wavelenghts) and appropriate fluorescence emission filters. With this system you can image live drosophila and zebrafish samples or use tissue clearing to perform optical sectioning deep into large fluorescent samples such as tissue sections, brains, embryos, organs, spheroids or biopsies. If you need higher resolution and have a smaller and thinner sample, check instead a confocal microscope such as the spinning disks [[3i_marianas_sdc|3i Marianas SDC]] and [[zeiss_cell_observer_sd|Zeiss Cell Observer SD]] or the point scanners [[zeiss_lsm_880|Zeiss LSM 880]] and [[zeiss_lsm_710|Zeiss LSM 710]]. A dedicated computer running [[https://​www.arivis.com/​en/​imaging-science/​arivis-vision4d|arivis Vision4D]] is available for working with multi-channel 2D, 3D and 4D images you generate in the system. ​ [[https://​imm.medicina.ulisboa.pt/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=lightsheet_illumination.jpg|Since only a single thin section of the sample is illuminated by the light sheet]], optical sectioning is achieved without a pinhole or image processing deconvolution. Light from the in-focus plane is collected by a sCMOS camera rather than pixel by pixel as in a point scanning laser confocal. This allows you to collect images faster and with less excitation light than you would with many other optical-sectioning microscopy techniques. You can image living samples in water or fixed samples where [[http://​blogs.zeiss.com/​microscopy/​news/​en/​references-for-clearing-protocols/​|tissue clearing]] has been performed. Samples are mounted not on coverslips but inside [[https://​imm.medicina.ulisboa.pt/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=lightsheet_chambers.jpg|special chambers]] that provide heating and cooling. The system is equipped with lasers from green to far red (488, 561 and 638 nm excitation wavelenghts) and appropriate fluorescence emission filters. With this system you can image live drosophila and zebrafish samples or use tissue clearing to perform optical sectioning deep into large fluorescent samples such as tissue sections, brains, embryos, organs, spheroids or biopsies. If you need higher resolution and have a smaller and thinner sample, check instead a confocal microscope such as the spinning disks [[3i_marianas_sdc|3i Marianas SDC]] and [[zeiss_cell_observer_sd|Zeiss Cell Observer SD]] or the point scanners [[zeiss_lsm_880|Zeiss LSM 880]] and [[zeiss_lsm_710|Zeiss LSM 710]]. A dedicated computer running [[https://​www.arivis.com/​en/​imaging-science/​arivis-vision4d|arivis Vision4D]] is available for working with multi-channel 2D, 3D and 4D images you generate in the system. ​
  
-{{info.gif?|}} Click on the image on the left to see the system beam path in higher detail+{{:info.png?25|}} Click on the image on the right to see the system beam path in higher detail
  
-{{warning.jpg?20|}} Data files older than **3 months** will be automatically deleted on this system, please copy your data to the iMM server using the desktop link.+{{:warning.png?25|}} Data files older than **3 months** will be automatically deleted on this system, please copy your data to the iMM server using the desktop link.
  
 ===== Additional information for sample preparation ===== ===== Additional information for sample preparation =====
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   * [[http://​applications.zeiss.com/​C125792900358A3F/​0/​E6504BFCBC3C2D39C1257BD500417CA6/​$FILE/​EN_41_011_058_LightsheetZ1_Sample-Preparation.pdf| Sample Preparation for Light Sheet Microscopy (white paper)]]   * [[http://​applications.zeiss.com/​C125792900358A3F/​0/​E6504BFCBC3C2D39C1257BD500417CA6/​$FILE/​EN_41_011_058_LightsheetZ1_Sample-Preparation.pdf| Sample Preparation for Light Sheet Microscopy (white paper)]]
   * [[https://​imm.medicina.ulisboa.pt/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=en_poster_overview-clearing-methods_rel-2.0.pdf|Clearing Methods in Microscopy (poster)]]   * [[https://​imm.medicina.ulisboa.pt/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=en_poster_overview-clearing-methods_rel-2.0.pdf|Clearing Methods in Microscopy (poster)]]
-  * [[http://blogs.zeiss.com/microscopy/news/en/references-for-clearing-protocols/|References ​for Tissue ​Clearing ​Protocols]]+  * [[https://applications.zeiss.com/C125792900358A3F/0/58052D27C3EAEA6AC1257E3C00401C75/$FILE/EN_41_013_097_Adjusting_refractive_index_for_clearing_applications_Ver02.pdf|Adjusting Refractive Index for Clearing ​Applications]] 
 +  * [[https://​applications.zeiss.com/​C125792900358A3F/​0/​D260E828348692A1C1257F570030EDC5/​$FILE/​EN_41_013_122_Imaging-Biological-Samples_a-Reference-List.pdf|Lightsheet Imaging Biological Samples – a Reference List]]
 ===== System components ===== ===== System components =====
  
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 ==== Objectives (Detection) ==== ==== Objectives (Detection) ====
  
-^  Magnification ​  ​^ ​ Model  ^  ​Type  ​^ ​ NA  ^  WD (mm)  ^ +^  Magnification ​  ​^ ​ Model  ^  ​Immersion ​ ​^ ​ NA  ^  WD (mm)  ​^ ​ Reference ​ ^ 
-|  20x  |  [[https://​www.micro-shop.zeiss.com/​?​s=10396935ab3601&​l=en&​p=uk&​f=o&​a=v&​m=s&​id=421452-9700-000|W Plan-Apochromat]]  |  Water  |  ​1.00  ​ ​2.4 ​ | +|  20x  ​| ​ W Plan-Apochromat DIC 75mm  |  Water  |  1.00  |  2.4  ​| ​ [[https://​www.micro-shop.zeiss.com/​en/​de/​shop/​objectives/​421452-9700-000/Objective-W-Plan-Apochromat-20x-1.0-DIC-M27-75mm#​|421452-9700-000]] ​ | 
-|  20x  |  Clr Plan-Neofluar Corr nd=1.45 ​ |  Clearing ​ |  1.00  |  5.6  |+|  20x  |  Clr Plan-Neofluar Corr nd=1.45 ​85mm  ​| ​ Clearing ​ |  1.00  |  5.6  ​| ​ [[https://​www.micro-shop.zeiss.com/​en/​de/​shop/​objectives/​421459-9970-000/​Objective-Clr-Plan-Neofluar-20x-1.0-Corr-nd-1.45-M32-85mm|421459-9970-000]] ​ |
  
 ==== Filtersets (Water) ==== ==== Filtersets (Water) ====
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 ===== If you want to use the system: ===== ===== If you want to use the system: =====
  
-  * Please contact the [[about|Bioimaging ​Facility]] (Ext: 47316). Sample chamber mounting and system initialization is performed by our staff.+  * Please contact the [[about|Bioimaging ​Unit]] (Ext: 47222). Sample chamber mounting and system initialization is performed by our staff.
  
 ---- ----
 [[resources|Back to the Resources page]] [[resources|Back to the Resources page]]
zeiss_lightsheet_z1.1570543862.txt.gz · Last modified: 2019/10/08 16:11 by bioimaging